Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method

The simplex method has been used to optimise the inulinase production by Aspergillus niger strain 13/36 with respect to 5 components of the medium. The extracellular enzyme activity was almost 80 U/mL, and over 1.6-fold higher than the results obtained by the classical »one-factor-at-a-time« method....

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Main Authors: Marcin Skowronek, Jan Fiedurek
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2004-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/163306
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author Marcin Skowronek
Jan Fiedurek
author_facet Marcin Skowronek
Jan Fiedurek
author_sort Marcin Skowronek
collection DOAJ
description The simplex method has been used to optimise the inulinase production by Aspergillus niger strain 13/36 with respect to 5 components of the medium. The extracellular enzyme activity was almost 80 U/mL, and over 1.6-fold higher than the results obtained by the classical »one-factor-at-a-time« method. The greatest advantage of the simplex method is that the optimum factor levels were determined in only 12 iterative steps, shortening a typical optimum search process considerably. This is the first use of the simplex method for optimising inulinase production.
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spelling doaj.art-49111b18e2f640f8973e2dbb6aca93632023-12-02T05:58:38ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062004-01-01423141146Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical MethodMarcin Skowronek0Jan Fiedurek1Department of Industrial Microbiology, Maria Curie-Sk³odowska University, Akademicka 19, PL-20-033 Lublin, PolandDepartment of Industrial Microbiology, Maria Curie-Sk³odowska University, Akademicka 19, PL-20-033 Lublin, PolandThe simplex method has been used to optimise the inulinase production by Aspergillus niger strain 13/36 with respect to 5 components of the medium. The extracellular enzyme activity was almost 80 U/mL, and over 1.6-fold higher than the results obtained by the classical »one-factor-at-a-time« method. The greatest advantage of the simplex method is that the optimum factor levels were determined in only 12 iterative steps, shortening a typical optimum search process considerably. This is the first use of the simplex method for optimising inulinase production.http://hrcak.srce.hr/file/163306simplex optimisationinulinaseAspergillus niger
spellingShingle Marcin Skowronek
Jan Fiedurek
Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method
Food Technology and Biotechnology
simplex optimisation
inulinase
Aspergillus niger
title Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method
title_full Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method
title_fullStr Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method
title_full_unstemmed Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method
title_short Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method
title_sort optimisation of inulinase production by aspergillus niger using simplex and classical method
topic simplex optimisation
inulinase
Aspergillus niger
url http://hrcak.srce.hr/file/163306
work_keys_str_mv AT marcinskowronek optimisationofinulinaseproductionbyaspergillusnigerusingsimplexandclassicalmethod
AT janfiedurek optimisationofinulinaseproductionbyaspergillusnigerusingsimplexandclassicalmethod