Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taisha...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4363904?pdf=render |
| _version_ | 1828899289750831104 |
|---|---|
| author | Shifa Yang Kai Wei Fengjuan Jia Xue Zhao Guolin Cui Fanxia Guo Ruiliang Zhu |
| author_facet | Shifa Yang Kai Wei Fengjuan Jia Xue Zhao Guolin Cui Fanxia Guo Ruiliang Zhu |
| author_sort | Shifa Yang |
| collection | DOAJ |
| description | Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation. Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography. The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively. Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2. TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion. By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity. TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro. Results indicated that TPPPS comprised three main components, among which, TPPPS1 mainly showed antioxidant effects, whereas TPPPS2 and TPPPS3 played key roles in immunomodulation, especially TPPPS3. Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator. |
| first_indexed | 2024-12-13T15:28:58Z |
| format | Article |
| id | doaj.art-49131eb7b6b9494f8d706b57ac3366de |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T15:28:58Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-49131eb7b6b9494f8d706b57ac3366de2022-12-21T23:40:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e011563810.1371/journal.pone.0115638Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.Shifa YangKai WeiFengjuan JiaXue ZhaoGuolin CuiFanxia GuoRuiliang ZhuTaishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation. Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography. The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively. Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2. TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion. By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity. TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro. Results indicated that TPPPS comprised three main components, among which, TPPPS1 mainly showed antioxidant effects, whereas TPPPS2 and TPPPS3 played key roles in immunomodulation, especially TPPPS3. Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.http://europepmc.org/articles/PMC4363904?pdf=render |
| spellingShingle | Shifa Yang Kai Wei Fengjuan Jia Xue Zhao Guolin Cui Fanxia Guo Ruiliang Zhu Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro. PLoS ONE |
| title | Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro. |
| title_full | Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro. |
| title_fullStr | Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro. |
| title_full_unstemmed | Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro. |
| title_short | Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro. |
| title_sort | characterization and biological activity of taishan pinus massoniana pollen polysaccharide in vitro |
| url | http://europepmc.org/articles/PMC4363904?pdf=render |
| work_keys_str_mv | AT shifayang characterizationandbiologicalactivityoftaishanpinusmassonianapollenpolysaccharideinvitro AT kaiwei characterizationandbiologicalactivityoftaishanpinusmassonianapollenpolysaccharideinvitro AT fengjuanjia characterizationandbiologicalactivityoftaishanpinusmassonianapollenpolysaccharideinvitro AT xuezhao characterizationandbiologicalactivityoftaishanpinusmassonianapollenpolysaccharideinvitro AT guolincui characterizationandbiologicalactivityoftaishanpinusmassonianapollenpolysaccharideinvitro AT fanxiaguo characterizationandbiologicalactivityoftaishanpinusmassonianapollenpolysaccharideinvitro AT ruiliangzhu characterizationandbiologicalactivityoftaishanpinusmassonianapollenpolysaccharideinvitro |