Expression levels of a gene signature in hiPSC associated with lung adenocarcinoma stem cells and its capability in eliciting specific antitumor immune‐response in a humanized mice model
Background Previous studies have reported that cancer stem cells (CSCs) play a key role in tumorigenesis, metastasis, and recurrence. CSC‐based vaccination confers better protection in tumor cells. However, isolation and cultivation of CSCs are difficult. This study aimed to explore the similarities...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-06-01
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| Series: | Thoracic Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1759-7714.13440 |
| _version_ | 1818047420901097472 |
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| author | Jingbo Wang Lijuan Shao Liujing Wu Wei Ma Yuanyuan Zheng Chaofeng Hu Furong Li |
| author_facet | Jingbo Wang Lijuan Shao Liujing Wu Wei Ma Yuanyuan Zheng Chaofeng Hu Furong Li |
| author_sort | Jingbo Wang |
| collection | DOAJ |
| description | Background Previous studies have reported that cancer stem cells (CSCs) play a key role in tumorigenesis, metastasis, and recurrence. CSC‐based vaccination confers better protection in tumor cells. However, isolation and cultivation of CSCs are difficult. This study aimed to explore the similarities between CSCs and induced pluripotent stem cells (iPSCs). Methods ALDH1+ cancer stem cells were isolated from lung adenocarcinoma patients and their gene expression patterns compared with human induced pluripotent stem cells (hiPSCs). In addition, a tumor vaccine was developed using hiPSC and unmethylated cytosine‐guanine (CpG). Finally, the antitumor properties of the vaccine were evaluated in a humanized mouse model. Results Preimmunization of iPSC+CpG elicited stronger antigen presentation and cytotoxic T cell response which suppressed the growth of tumors. Adoptive transfer of spleen T cells from the vaccine preimmunized mice inhibited tumor growth in unvaccinated recipients without any side effects. Conclusions This study suggests a universal strategy for tumor therapy which simplifies future clinical procedures. Therefore, the application of hiPSC elicits tumor protective responses. |
| first_indexed | 2024-12-10T10:05:31Z |
| format | Article |
| id | doaj.art-4917787520af4f32ae3bd9460affbe8c |
| institution | Directory Open Access Journal |
| issn | 1759-7706 1759-7714 |
| language | English |
| last_indexed | 2024-12-10T10:05:31Z |
| publishDate | 2020-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj.art-4917787520af4f32ae3bd9460affbe8c2022-12-22T01:53:15ZengWileyThoracic Cancer1759-77061759-77142020-06-011161603161210.1111/1759-7714.13440Expression levels of a gene signature in hiPSC associated with lung adenocarcinoma stem cells and its capability in eliciting specific antitumor immune‐response in a humanized mice modelJingbo Wang0Lijuan Shao1Liujing Wu2Wei Ma3Yuanyuan Zheng4Chaofeng Hu5Furong Li6Translational Medicine Collaborative Innovation Center, The Second Clinical Medical College (Shenzhen People's Hospital) Jinan University Shenzhen ChinaTranslational Medicine Collaborative Innovation Center, The Second Clinical Medical College (Shenzhen People's Hospital) Jinan University Shenzhen ChinaTranslational Medicine Collaborative Innovation Center, The Second Clinical Medical College (Shenzhen People's Hospital) Jinan University Shenzhen ChinaTranslational Medicine Collaborative Innovation Center, The Second Clinical Medical College (Shenzhen People's Hospital) Jinan University Shenzhen ChinaTranslational Medicine Collaborative Innovation Center, The Second Clinical Medical College (Shenzhen People's Hospital) Jinan University Shenzhen ChinaIntegrated Chinese and Western Medicine Postdoctoral research station Jinan University Guangzhou ChinaTranslational Medicine Collaborative Innovation Center, The Second Clinical Medical College (Shenzhen People's Hospital) Jinan University Shenzhen ChinaBackground Previous studies have reported that cancer stem cells (CSCs) play a key role in tumorigenesis, metastasis, and recurrence. CSC‐based vaccination confers better protection in tumor cells. However, isolation and cultivation of CSCs are difficult. This study aimed to explore the similarities between CSCs and induced pluripotent stem cells (iPSCs). Methods ALDH1+ cancer stem cells were isolated from lung adenocarcinoma patients and their gene expression patterns compared with human induced pluripotent stem cells (hiPSCs). In addition, a tumor vaccine was developed using hiPSC and unmethylated cytosine‐guanine (CpG). Finally, the antitumor properties of the vaccine were evaluated in a humanized mouse model. Results Preimmunization of iPSC+CpG elicited stronger antigen presentation and cytotoxic T cell response which suppressed the growth of tumors. Adoptive transfer of spleen T cells from the vaccine preimmunized mice inhibited tumor growth in unvaccinated recipients without any side effects. Conclusions This study suggests a universal strategy for tumor therapy which simplifies future clinical procedures. Therefore, the application of hiPSC elicits tumor protective responses.https://doi.org/10.1111/1759-7714.13440Cancer stem cellhiPSChumanize mice modelimmune responsetumor vaccine |
| spellingShingle | Jingbo Wang Lijuan Shao Liujing Wu Wei Ma Yuanyuan Zheng Chaofeng Hu Furong Li Expression levels of a gene signature in hiPSC associated with lung adenocarcinoma stem cells and its capability in eliciting specific antitumor immune‐response in a humanized mice model Thoracic Cancer Cancer stem cell hiPSC humanize mice model immune response tumor vaccine |
| title | Expression levels of a gene signature in hiPSC associated with lung adenocarcinoma stem cells and its capability in eliciting specific antitumor immune‐response in a humanized mice model |
| title_full | Expression levels of a gene signature in hiPSC associated with lung adenocarcinoma stem cells and its capability in eliciting specific antitumor immune‐response in a humanized mice model |
| title_fullStr | Expression levels of a gene signature in hiPSC associated with lung adenocarcinoma stem cells and its capability in eliciting specific antitumor immune‐response in a humanized mice model |
| title_full_unstemmed | Expression levels of a gene signature in hiPSC associated with lung adenocarcinoma stem cells and its capability in eliciting specific antitumor immune‐response in a humanized mice model |
| title_short | Expression levels of a gene signature in hiPSC associated with lung adenocarcinoma stem cells and its capability in eliciting specific antitumor immune‐response in a humanized mice model |
| title_sort | expression levels of a gene signature in hipsc associated with lung adenocarcinoma stem cells and its capability in eliciting specific antitumor immune response in a humanized mice model |
| topic | Cancer stem cell hiPSC humanize mice model immune response tumor vaccine |
| url | https://doi.org/10.1111/1759-7714.13440 |
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