Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda
Context: Bioprospection has become a dynamic scientific field that explores novel possibilities for the implementation of natural products in medicine and pharmacy. Compared to marine species from all kingdoms, freshwater species have been highly neglected. Objective: This work focuses on the screen...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2017.1297468 |
| _version_ | 1819060478806065152 |
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| author | Aleksandra Talevska Boris Pejin Tanja Beric Slavisa Stankovic |
| author_facet | Aleksandra Talevska Boris Pejin Tanja Beric Slavisa Stankovic |
| author_sort | Aleksandra Talevska |
| collection | DOAJ |
| description | Context: Bioprospection has become a dynamic scientific field that explores novel possibilities for the implementation of natural products in medicine and pharmacy. Compared to marine species from all kingdoms, freshwater species have been highly neglected. Objective: This work focuses on the screening of acetylcholinesterase inhibitory (AChE) and mutagenic activities of the acetone extract (obtained by maceration) of the freshwater sponge Ochridaspongia rotunda Arndt (Malawispongiidae) in vitro. Materials and methods: AChE inhibitory activity was evaluated both in liquid (five different concentrations of the extract, from 1 to 100 μg/mL) and in solid (seven different concentrations of the extract, from 0.5 to 10.0 μg) by methods well described in literature, while mutagenicity was estimated using the Ames test (four different concentrations of the extract, from 0.106 to 1.328 mg/plate). Results: Ochridaspongia rotunda acetone extract exhibited promising AChE inhibitory activity in a dose-dependent manner both in liquid (IC50 23.07 μg/mL) and in solid (1.50 μg). Furthermore, the Ames test revealed no sign of mutagenicity at any concentration tested. Its FTIR spectrum coupled with the positive Liebermann?Burchard, Salkowski and Zak color reactions (tests) indicated the presence of sterol compounds. Discussion and conclusion: The screened extract may inspire a search for novel anticholinesterase therapeutic agent(s) potentially used in the treatment of Alzheimer's disease. Further research will be directed toward its detailed chemical analysis along with addressing the issue of a real producer of the natural product(s) responsible for the AChE activity observed. |
| first_indexed | 2024-12-21T14:27:38Z |
| format | Article |
| id | doaj.art-4919f04b4d804cc894be5da33a25ae16 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-21T14:27:38Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-4919f04b4d804cc894be5da33a25ae162022-12-21T19:00:35ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015511313131610.1080/13880209.2017.12974681297468Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotundaAleksandra Talevska0Boris Pejin1Tanja Beric2Slavisa Stankovic3University of St. Clement of Ohrid, Hydrobiological InstituteInstitute for Multidisciplinary Research – IMSI, University of BelgradeUniversity of BelgradeUniversity of BelgradeContext: Bioprospection has become a dynamic scientific field that explores novel possibilities for the implementation of natural products in medicine and pharmacy. Compared to marine species from all kingdoms, freshwater species have been highly neglected. Objective: This work focuses on the screening of acetylcholinesterase inhibitory (AChE) and mutagenic activities of the acetone extract (obtained by maceration) of the freshwater sponge Ochridaspongia rotunda Arndt (Malawispongiidae) in vitro. Materials and methods: AChE inhibitory activity was evaluated both in liquid (five different concentrations of the extract, from 1 to 100 μg/mL) and in solid (seven different concentrations of the extract, from 0.5 to 10.0 μg) by methods well described in literature, while mutagenicity was estimated using the Ames test (four different concentrations of the extract, from 0.106 to 1.328 mg/plate). Results: Ochridaspongia rotunda acetone extract exhibited promising AChE inhibitory activity in a dose-dependent manner both in liquid (IC50 23.07 μg/mL) and in solid (1.50 μg). Furthermore, the Ames test revealed no sign of mutagenicity at any concentration tested. Its FTIR spectrum coupled with the positive Liebermann?Burchard, Salkowski and Zak color reactions (tests) indicated the presence of sterol compounds. Discussion and conclusion: The screened extract may inspire a search for novel anticholinesterase therapeutic agent(s) potentially used in the treatment of Alzheimer's disease. Further research will be directed toward its detailed chemical analysis along with addressing the issue of a real producer of the natural product(s) responsible for the AChE activity observed.http://dx.doi.org/10.1080/13880209.2017.1297468bioprospectionmalawispongiidaeachealzheimer's disease |
| spellingShingle | Aleksandra Talevska Boris Pejin Tanja Beric Slavisa Stankovic Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda Pharmaceutical Biology bioprospection malawispongiidae ache alzheimer's disease |
| title | Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda |
| title_full | Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda |
| title_fullStr | Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda |
| title_full_unstemmed | Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda |
| title_short | Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda |
| title_sort | further insight into the bioactivity of the freshwater sponge ochridaspongia rotunda |
| topic | bioprospection malawispongiidae ache alzheimer's disease |
| url | http://dx.doi.org/10.1080/13880209.2017.1297468 |
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