Ethyl Acetate Fraction and Isolated Phenolics Derivatives from <i>Mandevilla moricandiana</i> Identified by UHPLC-DAD-ESI-MS<sup>n</sup> with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction
Endothelial dysfunction in obesity plays a key role in the development of cardiovascular diseases, and it is characterized by increased vascular tonus and oxidative stress. Thus, this study aimed to investigate the vasodilatory and antioxidant activities of <i>Mandevilla moricandiana</i>...
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| author | Leticia L. D. M. Ferreira Valéria de F. Leão Cinthya M. de Melo Thelma de B. Machado Ana Claudia F. Amaral Leandro L. da Silva Naomi K. Simas Michelle F. Muzitano Ivana C. R. Leal Juliana M. Raimundo |
| author_facet | Leticia L. D. M. Ferreira Valéria de F. Leão Cinthya M. de Melo Thelma de B. Machado Ana Claudia F. Amaral Leandro L. da Silva Naomi K. Simas Michelle F. Muzitano Ivana C. R. Leal Juliana M. Raimundo |
| author_sort | Leticia L. D. M. Ferreira |
| collection | DOAJ |
| description | Endothelial dysfunction in obesity plays a key role in the development of cardiovascular diseases, and it is characterized by increased vascular tonus and oxidative stress. Thus, this study aimed to investigate the vasodilatory and antioxidant activities of <i>Mandevilla moricandiana</i> ethyl acetate fraction and subfractions. Vascular effects were investigated on aorta isolated from control and monosodium glutamate (MSG) induced-obese Wistar rats, and antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) methods. The ethyl acetate fraction (MMEAF) induced a concentration-dependent vasodilation on aortic rings through the NO pathway, with the involvement of histamine H1 and estrogen ERα receptors and showed potent antioxidant activity. In aorta of MSG obese rats, maximal relaxation to acetylcholine was increased in the presence of MMEAF (3 µg/mL), indicating that MMEAF ameliorated obesity-induced endothelial dysfunction. Quercetin and kaempferol aglycones and their correspondent glycosides, as well as caffeoylquinic acid derivatives, A-type procyanidin trimer, ursolic and oleanolic triterpenoid acids were identified in subfractions from MMEAF and seem to be the metabolites responsible for the vascular and antioxidant activities of this fraction. |
| first_indexed | 2024-03-10T08:29:09Z |
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| id | doaj.art-491a782dda4340b08cb30046ee389d05 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T08:29:09Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
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| series | Pharmaceutics |
| spelling | doaj.art-491a782dda4340b08cb30046ee389d052023-11-22T09:13:45ZengMDPI AGPharmaceutics1999-49232021-07-01138117310.3390/pharmaceutics13081173Ethyl Acetate Fraction and Isolated Phenolics Derivatives from <i>Mandevilla moricandiana</i> Identified by UHPLC-DAD-ESI-MS<sup>n</sup> with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial DysfunctionLeticia L. D. M. Ferreira0Valéria de F. Leão1Cinthya M. de Melo2Thelma de B. Machado3Ana Claudia F. Amaral4Leandro L. da Silva5Naomi K. Simas6Michelle F. Muzitano7Ivana C. R. Leal8Juliana M. Raimundo9Pharmacology of Bioactive Products Research Group, Federal University of Rio de Janeiro—Macaé Campus, Macaé 27930-560, RJ, BrazilPharmacology of Bioactive Products Research Group, Federal University of Rio de Janeiro—Macaé Campus, Macaé 27930-560, RJ, BrazilPharmacology of Bioactive Products Research Group, Federal University of Rio de Janeiro—Macaé Campus, Macaé 27930-560, RJ, BrazilLAQV-REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalLaboratory of Medicinal Plants and Derivatives, Farmanguinhos, Oswaldo Cruz Foundation, Rio de Janeiro 21041-250, RJ, BrazilPharmacology of Bioactive Products Research Group, Federal University of Rio de Janeiro—Macaé Campus, Macaé 27930-560, RJ, BrazilLaboratory of Natural Products and Biological Assays, Pharmacy Faculty, Health Sciences Center, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilLaboratory of Bioactive Products, Federal University of Rio de Janeiro—Macaé Campus, Macaé 27933-378, RJ, BrazilLaboratory of Natural Products and Biological Assays, Pharmacy Faculty, Health Sciences Center, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilPharmacology of Bioactive Products Research Group, Federal University of Rio de Janeiro—Macaé Campus, Macaé 27930-560, RJ, BrazilEndothelial dysfunction in obesity plays a key role in the development of cardiovascular diseases, and it is characterized by increased vascular tonus and oxidative stress. Thus, this study aimed to investigate the vasodilatory and antioxidant activities of <i>Mandevilla moricandiana</i> ethyl acetate fraction and subfractions. Vascular effects were investigated on aorta isolated from control and monosodium glutamate (MSG) induced-obese Wistar rats, and antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) methods. The ethyl acetate fraction (MMEAF) induced a concentration-dependent vasodilation on aortic rings through the NO pathway, with the involvement of histamine H1 and estrogen ERα receptors and showed potent antioxidant activity. In aorta of MSG obese rats, maximal relaxation to acetylcholine was increased in the presence of MMEAF (3 µg/mL), indicating that MMEAF ameliorated obesity-induced endothelial dysfunction. Quercetin and kaempferol aglycones and their correspondent glycosides, as well as caffeoylquinic acid derivatives, A-type procyanidin trimer, ursolic and oleanolic triterpenoid acids were identified in subfractions from MMEAF and seem to be the metabolites responsible for the vascular and antioxidant activities of this fraction.https://www.mdpi.com/1999-4923/13/8/1173<i>Mandevilla moricandiana</i>polyphenolsflavonolsprocyanidintriterpenesvasodilation |
| spellingShingle | Leticia L. D. M. Ferreira Valéria de F. Leão Cinthya M. de Melo Thelma de B. Machado Ana Claudia F. Amaral Leandro L. da Silva Naomi K. Simas Michelle F. Muzitano Ivana C. R. Leal Juliana M. Raimundo Ethyl Acetate Fraction and Isolated Phenolics Derivatives from <i>Mandevilla moricandiana</i> Identified by UHPLC-DAD-ESI-MS<sup>n</sup> with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction Pharmaceutics <i>Mandevilla moricandiana</i> polyphenols flavonols procyanidin triterpenes vasodilation |
| title | Ethyl Acetate Fraction and Isolated Phenolics Derivatives from <i>Mandevilla moricandiana</i> Identified by UHPLC-DAD-ESI-MS<sup>n</sup> with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction |
| title_full | Ethyl Acetate Fraction and Isolated Phenolics Derivatives from <i>Mandevilla moricandiana</i> Identified by UHPLC-DAD-ESI-MS<sup>n</sup> with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction |
| title_fullStr | Ethyl Acetate Fraction and Isolated Phenolics Derivatives from <i>Mandevilla moricandiana</i> Identified by UHPLC-DAD-ESI-MS<sup>n</sup> with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction |
| title_full_unstemmed | Ethyl Acetate Fraction and Isolated Phenolics Derivatives from <i>Mandevilla moricandiana</i> Identified by UHPLC-DAD-ESI-MS<sup>n</sup> with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction |
| title_short | Ethyl Acetate Fraction and Isolated Phenolics Derivatives from <i>Mandevilla moricandiana</i> Identified by UHPLC-DAD-ESI-MS<sup>n</sup> with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction |
| title_sort | ethyl acetate fraction and isolated phenolics derivatives from i mandevilla moricandiana i identified by uhplc dad esi ms sup n sup with pharmacological potential for the improvement of obesity induced endothelial dysfunction |
| topic | <i>Mandevilla moricandiana</i> polyphenols flavonols procyanidin triterpenes vasodilation |
| url | https://www.mdpi.com/1999-4923/13/8/1173 |
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