Evaluation of Vancomycin Minimum Inhibitory Concentration in the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA)
Staphylococcus aureus (S.aureus) and coagulase negative Staphylococci (CONS) are the commonest pathogens that lead to severe bacterial infections. It is a bacterium with consistent resistance development against commonly used antibiotics, with emergence of Methicillin resistant staphylococcus aureus...
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Journal of Pure and Applied Microbiology
2019-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/evaluation-of-vancomycin-minimum-inhibitory-concentration-in-the-clinical-isolates-of-methicillin-resistant-staphylococcus-aureus-mrsa/ |
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| author | Anitha T.K. Morubagal Raghavendra Rao Ranjitha Shankaregowda Rashmi P. Mahale Sowmya G.S. Vidyavathi B. Chitharagi |
| author_facet | Anitha T.K. Morubagal Raghavendra Rao Ranjitha Shankaregowda Rashmi P. Mahale Sowmya G.S. Vidyavathi B. Chitharagi |
| author_sort | Anitha T.K. |
| collection | DOAJ |
| description | Staphylococcus aureus (S.aureus) and coagulase negative Staphylococci (CONS) are the commonest pathogens that lead to severe bacterial infections. It is a bacterium with consistent resistance development against commonly used antibiotics, with emergence of Methicillin resistant staphylococcus aureus (MRSA) causing several infections in patients following hospitalization. Glycopeptides like vancomycin is used as primary drug for treating infectious diseases caused by MRSA. Due to indiscriminate use of vancomycin to treat MRSA, several strains with variable susceptibility to the same have emerged. Evaluation of Vancomycin Minimum Inhibitory Concentration (MIC) in the MRSA isolates obtained from clinical samples received in the diagnostic microbiology laboratory. About 120 Staphylococci obtained from different clinical samples in the diagnostic Microbiology laboratory, at tertiary health care center, South India, were included in the study. The isolates were identified and susceptibility to the relevant antibiotics was done by Vitek 2 an automated system. Vancomycin MIC was detected by Vitek 2 and E-test strip technique. Out of 120 Staphylococcal strains, 79(65.8%) S. aureus and 41(34.1%) CONS were isolated. Methicillin resistance was observed in 38 (48.1%) strains of S. aureus. Almost all 38 MRSA isolates were vancomycin sensitive with MIC range of 0.5 - 2µg/ml. Maximum isolates had MIC of 1 µg/ml i.e. 65.78% and 71% by E-Test and Vitek 2 respectively. The reported increased MIC of Vancomycin, though within the susceptible range, might experience poor clinical outcomes. Emergence and spread of resistance to glycopeptides like vancomycin needs to be kept in check by rapidly detecting the strains for resistance and strictly obeying the infection control practices. |
| first_indexed | 2024-12-18T11:35:16Z |
| format | Article |
| id | doaj.art-491c02ea36f74d8591f3489cddf6759f |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-18T11:35:16Z |
| publishDate | 2019-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
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| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-491c02ea36f74d8591f3489cddf6759f2022-12-21T21:09:31ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2019-09-011331797180110.22207/JPAM.13.3.56Evaluation of Vancomycin Minimum Inhibitory Concentration in the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA)Anitha T.K.0Morubagal Raghavendra Rao1Ranjitha Shankaregowda2Rashmi P. Mahale3Sowmya G.S.4Vidyavathi B. Chitharagi51Department of Microbiology, Kanachur Institute of Medical Sciences, Mangalore, India.2Department of Microbiology, JSSMC, JSSAHER, S.S. Nagar, Mysore - 570 015, India. 2Department of Microbiology, JSSMC, JSSAHER, S.S. Nagar, Mysore - 570 015, India. 2Department of Microbiology, JSSMC, JSSAHER, S.S. Nagar, Mysore - 570 015, India. 2Department of Microbiology, JSSMC, JSSAHER, S.S. Nagar, Mysore - 570 015, India. 2Department of Microbiology, JSSMC, JSSAHER, S.S. Nagar, Mysore - 570 015, India. Staphylococcus aureus (S.aureus) and coagulase negative Staphylococci (CONS) are the commonest pathogens that lead to severe bacterial infections. It is a bacterium with consistent resistance development against commonly used antibiotics, with emergence of Methicillin resistant staphylococcus aureus (MRSA) causing several infections in patients following hospitalization. Glycopeptides like vancomycin is used as primary drug for treating infectious diseases caused by MRSA. Due to indiscriminate use of vancomycin to treat MRSA, several strains with variable susceptibility to the same have emerged. Evaluation of Vancomycin Minimum Inhibitory Concentration (MIC) in the MRSA isolates obtained from clinical samples received in the diagnostic microbiology laboratory. About 120 Staphylococci obtained from different clinical samples in the diagnostic Microbiology laboratory, at tertiary health care center, South India, were included in the study. The isolates were identified and susceptibility to the relevant antibiotics was done by Vitek 2 an automated system. Vancomycin MIC was detected by Vitek 2 and E-test strip technique. Out of 120 Staphylococcal strains, 79(65.8%) S. aureus and 41(34.1%) CONS were isolated. Methicillin resistance was observed in 38 (48.1%) strains of S. aureus. Almost all 38 MRSA isolates were vancomycin sensitive with MIC range of 0.5 - 2µg/ml. Maximum isolates had MIC of 1 µg/ml i.e. 65.78% and 71% by E-Test and Vitek 2 respectively. The reported increased MIC of Vancomycin, though within the susceptible range, might experience poor clinical outcomes. Emergence and spread of resistance to glycopeptides like vancomycin needs to be kept in check by rapidly detecting the strains for resistance and strictly obeying the infection control practices.https://microbiologyjournal.org/evaluation-of-vancomycin-minimum-inhibitory-concentration-in-the-clinical-isolates-of-methicillin-resistant-staphylococcus-aureus-mrsa/methicillin resistant staphylococcus aureus (mrsa)vancomycinminimum inhibitory concentration (mic)vitek 2e-test strip technique. |
| spellingShingle | Anitha T.K. Morubagal Raghavendra Rao Ranjitha Shankaregowda Rashmi P. Mahale Sowmya G.S. Vidyavathi B. Chitharagi Evaluation of Vancomycin Minimum Inhibitory Concentration in the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA) Journal of Pure and Applied Microbiology methicillin resistant staphylococcus aureus (mrsa) vancomycin minimum inhibitory concentration (mic) vitek 2 e-test strip technique. |
| title | Evaluation of Vancomycin Minimum Inhibitory Concentration in the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_full | Evaluation of Vancomycin Minimum Inhibitory Concentration in the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_fullStr | Evaluation of Vancomycin Minimum Inhibitory Concentration in the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_full_unstemmed | Evaluation of Vancomycin Minimum Inhibitory Concentration in the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_short | Evaluation of Vancomycin Minimum Inhibitory Concentration in the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_sort | evaluation of vancomycin minimum inhibitory concentration in the clinical isolates of methicillin resistant staphylococcus aureus mrsa |
| topic | methicillin resistant staphylococcus aureus (mrsa) vancomycin minimum inhibitory concentration (mic) vitek 2 e-test strip technique. |
| url | https://microbiologyjournal.org/evaluation-of-vancomycin-minimum-inhibitory-concentration-in-the-clinical-isolates-of-methicillin-resistant-staphylococcus-aureus-mrsa/ |
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