Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil
The aims of this study were to determine the occurrence ofAnaplasma platys and Ehrlichia canisinfection in dogs in Porto Alegre, Southern Brazil; and to investigate their association with hematological abnormalities. Serum samples from 196 dogs were first tested using dot-ELISA for antibodies agains...
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Colégio Brasileiro de Parasitologia Veterinaria
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000300360&lng=en&tlng=en |
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| author | Camila Serina Lasta Andrea Pires dos Santos Joanne Belle Messick Simone Tostes Oliveira Alexander Welker Biondo Rafael Felipe da Costa Vieira Magnus Larruscaim Dalmolin Félix Hilario Diaz González |
| author_facet | Camila Serina Lasta Andrea Pires dos Santos Joanne Belle Messick Simone Tostes Oliveira Alexander Welker Biondo Rafael Felipe da Costa Vieira Magnus Larruscaim Dalmolin Félix Hilario Diaz González |
| author_sort | Camila Serina Lasta |
| collection | DOAJ |
| description | The aims of this study were to determine the occurrence ofAnaplasma platys and Ehrlichia canisinfection in dogs in Porto Alegre, Southern Brazil; and to investigate their association with hematological abnormalities. Serum samples from 196 dogs were first tested using dot-ELISA for antibodies against Anaplasmaspp. and Ehrlichia canis. Peripheral blood samples from 199 dogs were subjected to 16S rRNA nested PCR (nPCR) for A. platysand E. canis, followed by DNA sequencing to ensure pathogen identity. A total of 19/196 samples (9.69%) were positive forAnaplasma spp. using ELISA and 28/199 (14.07%) samples were positive for A. platys by nested PCR. All the dog samples were negative for E. canis, both in anti-E. canisantibody tests and in nested PCR. There were no significant differences in hematological parameters between A. platys-PCR positive and negative dogs and Anaplasma spp. serologically positive dogs, except for basophil counts, which were higher in nPCR-positive dogs. This is the first report showing A. platys presence in dogs in Southern Brazil. In conclusion, hematological parameters may not be sufficient to diagnose A. platys infection in dogs in Southern Brazil, probably due either to low pathogenicity or to chronic infection. On the other hand, E. canis may either have very low occurrence or be absent in dogs in Porto Alegre. |
| first_indexed | 2024-12-13T22:01:45Z |
| format | Article |
| id | doaj.art-491f108399034e8987e58d69adacdc98 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-13T22:01:45Z |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-491f108399034e8987e58d69adacdc982022-12-21T23:29:58ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-296122336036610.1590/S1984-29612013000300007S1984-29612013000300360Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern BrazilCamila Serina LastaAndrea Pires dos SantosJoanne Belle MessickSimone Tostes OliveiraAlexander Welker BiondoRafael Felipe da Costa VieiraMagnus Larruscaim DalmolinFélix Hilario Diaz GonzálezThe aims of this study were to determine the occurrence ofAnaplasma platys and Ehrlichia canisinfection in dogs in Porto Alegre, Southern Brazil; and to investigate their association with hematological abnormalities. Serum samples from 196 dogs were first tested using dot-ELISA for antibodies against Anaplasmaspp. and Ehrlichia canis. Peripheral blood samples from 199 dogs were subjected to 16S rRNA nested PCR (nPCR) for A. platysand E. canis, followed by DNA sequencing to ensure pathogen identity. A total of 19/196 samples (9.69%) were positive forAnaplasma spp. using ELISA and 28/199 (14.07%) samples were positive for A. platys by nested PCR. All the dog samples were negative for E. canis, both in anti-E. canisantibody tests and in nested PCR. There were no significant differences in hematological parameters between A. platys-PCR positive and negative dogs and Anaplasma spp. serologically positive dogs, except for basophil counts, which were higher in nPCR-positive dogs. This is the first report showing A. platys presence in dogs in Southern Brazil. In conclusion, hematological parameters may not be sufficient to diagnose A. platys infection in dogs in Southern Brazil, probably due either to low pathogenicity or to chronic infection. On the other hand, E. canis may either have very low occurrence or be absent in dogs in Porto Alegre.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000300360&lng=en&tlng=enAnaplasmataceaedogsSouthern Brazil16S rRNA gene |
| spellingShingle | Camila Serina Lasta Andrea Pires dos Santos Joanne Belle Messick Simone Tostes Oliveira Alexander Welker Biondo Rafael Felipe da Costa Vieira Magnus Larruscaim Dalmolin Félix Hilario Diaz González Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil Revista Brasileira de Parasitologia Veterinária Anaplasmataceae dogs Southern Brazil 16S rRNA gene |
| title | Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil |
| title_full | Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil |
| title_fullStr | Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil |
| title_full_unstemmed | Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil |
| title_short | Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil |
| title_sort | molecular detection of ehrlichia canisand anaplasma platys in dogs in southern brazil |
| topic | Anaplasmataceae dogs Southern Brazil 16S rRNA gene |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000300360&lng=en&tlng=en |
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