Transcriptome Signatures of Canine Mammary Gland Tumors and Its Comparison to Human Breast Cancers
Breast cancer (BC)/mammary gland carcinoma (MGC) is the most frequently diagnosed and leading cause of cancer-related mortality in both women and canines. To better understand both canine MGC and human BC-specific genes, we sequenced RNAs obtained from eight pairs of carcinomas and adjacent normal t...
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| Format: | Article |
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MDPI AG
2018-09-01
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| Series: | Cancers |
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| Online Access: | http://www.mdpi.com/2072-6694/10/9/317 |
| _version_ | 1797711709761175552 |
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| author | Kang-Hoon Lee Hyoung-Min Park Keun-Hong Son Tae-Jin Shin Je-Yoel Cho |
| author_facet | Kang-Hoon Lee Hyoung-Min Park Keun-Hong Son Tae-Jin Shin Je-Yoel Cho |
| author_sort | Kang-Hoon Lee |
| collection | DOAJ |
| description | Breast cancer (BC)/mammary gland carcinoma (MGC) is the most frequently diagnosed and leading cause of cancer-related mortality in both women and canines. To better understand both canine MGC and human BC-specific genes, we sequenced RNAs obtained from eight pairs of carcinomas and adjacent normal tissues in dogs. By comprehensive transcriptome analysis, 351 differentially expressed genes (DEGs) were identified in overall canine MGCs. Based on the DEGs, comparative analysis revealed correlation existing among the three histological subtypes of canine MGC (ductal, simple, and complex) and four molecular subtypes of human BC (HER2+, ER+, ER&HER2+, and TNBC). Eight DEGs shared by all three subtypes of canine MGCs had been previously reported as cancer-associated genes in human studies. Gene ontology and pathway analyses using the identified DEGs revealed that the biological processes of cell proliferation, adhesion, and inflammatory responses are enriched in up-regulated MGC DEGs. In contrast, fatty acid homeostasis and transcription regulation involved in cell fate commitment were down-regulated in MGC DEGs. Moreover, correlations are demonstrated between upstream promoter transcripts and DEGs. Canine MGC- and subtype-enriched gene expression allows us to better understand both human BC and canine MGC, yielding new insight into the development of biomarkers and targets for both diseases. |
| first_indexed | 2024-03-12T07:11:03Z |
| format | Article |
| id | doaj.art-49230c0e7b55445f8cffcaa46cb56e2a |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T07:11:03Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-49230c0e7b55445f8cffcaa46cb56e2a2023-09-02T23:06:22ZengMDPI AGCancers2072-66942018-09-0110931710.3390/cancers10090317cancers10090317Transcriptome Signatures of Canine Mammary Gland Tumors and Its Comparison to Human Breast CancersKang-Hoon Lee0Hyoung-Min Park1Keun-Hong Son2Tae-Jin Shin3Je-Yoel Cho4Department of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaDepartment of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaDepartment of Microbiology, College of Natural Sciences, Dankook University, Cheonan 31116, KoreaDepartment of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaDepartment of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaBreast cancer (BC)/mammary gland carcinoma (MGC) is the most frequently diagnosed and leading cause of cancer-related mortality in both women and canines. To better understand both canine MGC and human BC-specific genes, we sequenced RNAs obtained from eight pairs of carcinomas and adjacent normal tissues in dogs. By comprehensive transcriptome analysis, 351 differentially expressed genes (DEGs) were identified in overall canine MGCs. Based on the DEGs, comparative analysis revealed correlation existing among the three histological subtypes of canine MGC (ductal, simple, and complex) and four molecular subtypes of human BC (HER2+, ER+, ER&HER2+, and TNBC). Eight DEGs shared by all three subtypes of canine MGCs had been previously reported as cancer-associated genes in human studies. Gene ontology and pathway analyses using the identified DEGs revealed that the biological processes of cell proliferation, adhesion, and inflammatory responses are enriched in up-regulated MGC DEGs. In contrast, fatty acid homeostasis and transcription regulation involved in cell fate commitment were down-regulated in MGC DEGs. Moreover, correlations are demonstrated between upstream promoter transcripts and DEGs. Canine MGC- and subtype-enriched gene expression allows us to better understand both human BC and canine MGC, yielding new insight into the development of biomarkers and targets for both diseases.http://www.mdpi.com/2072-6694/10/9/317transcriptomedogmammary gland tumorbreast cancerRNA-seq |
| spellingShingle | Kang-Hoon Lee Hyoung-Min Park Keun-Hong Son Tae-Jin Shin Je-Yoel Cho Transcriptome Signatures of Canine Mammary Gland Tumors and Its Comparison to Human Breast Cancers Cancers transcriptome dog mammary gland tumor breast cancer RNA-seq |
| title | Transcriptome Signatures of Canine Mammary Gland Tumors and Its Comparison to Human Breast Cancers |
| title_full | Transcriptome Signatures of Canine Mammary Gland Tumors and Its Comparison to Human Breast Cancers |
| title_fullStr | Transcriptome Signatures of Canine Mammary Gland Tumors and Its Comparison to Human Breast Cancers |
| title_full_unstemmed | Transcriptome Signatures of Canine Mammary Gland Tumors and Its Comparison to Human Breast Cancers |
| title_short | Transcriptome Signatures of Canine Mammary Gland Tumors and Its Comparison to Human Breast Cancers |
| title_sort | transcriptome signatures of canine mammary gland tumors and its comparison to human breast cancers |
| topic | transcriptome dog mammary gland tumor breast cancer RNA-seq |
| url | http://www.mdpi.com/2072-6694/10/9/317 |
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