Ginsenoside Rg3 inhibits pulmonary fibrosis by preventing HIF-1α nuclear localisation
Abstract Background Excessive fibroblast proliferation during pulmonary fibrosis leads to structural abnormalities in lung tissue and causes hypoxia and cell injury. However, the mechanisms and effective treatment are still limited. Methods In vivo, we used bleomycin to induce pulmonary fibrosis in...
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Format: | Article |
Language: | English |
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BMC
2021-02-01
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Series: | BMC Pulmonary Medicine |
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Online Access: | https://doi.org/10.1186/s12890-021-01426-5 |
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author | Zhuo Fu Yong-sheng Xu Chun-quan Cai |
author_facet | Zhuo Fu Yong-sheng Xu Chun-quan Cai |
author_sort | Zhuo Fu |
collection | DOAJ |
description | Abstract Background Excessive fibroblast proliferation during pulmonary fibrosis leads to structural abnormalities in lung tissue and causes hypoxia and cell injury. However, the mechanisms and effective treatment are still limited. Methods In vivo, we used bleomycin to induce pulmonary fibrosis in mice. IHC and Masson staining were used to evaluate the inhibitory effect of ginsenoside Rg3 in pulmonary fibrosis. In vitro, scanning electron microscopy, transwell and wound healing were used to evaluate the cell phenotype of LL 29 cells. In addition, biacore was used to detect the binding between ginsenoside Rg3 and HIF-1α. Results Here, we found that bleomycin induces the activation of the HIF-1α/TGFβ1 signalling pathway and further enhances the migration and proliferation of fibroblasts through the epithelial mesenchymal transition (EMT). In addition, molecular docking and biacore results indicated that ginsenoside Rg3 can bind HIF-1α. Therefore, Ginsenoside Rg3 can slow down the progression of pulmonary fibrosis by inhibiting the nuclear localisation of HIF-1α. Conclusions This finding suggests that early targeted treatment of hypoxia may have potential value in the treatment of pulmonary fibrosis. |
first_indexed | 2024-12-16T16:34:19Z |
format | Article |
id | doaj.art-492461a1c8db47d1a940966312e79278 |
institution | Directory Open Access Journal |
issn | 1471-2466 |
language | English |
last_indexed | 2024-12-16T16:34:19Z |
publishDate | 2021-02-01 |
publisher | BMC |
record_format | Article |
series | BMC Pulmonary Medicine |
spelling | doaj.art-492461a1c8db47d1a940966312e792782022-12-21T22:24:31ZengBMCBMC Pulmonary Medicine1471-24662021-02-0121111010.1186/s12890-021-01426-5Ginsenoside Rg3 inhibits pulmonary fibrosis by preventing HIF-1α nuclear localisationZhuo Fu0Yong-sheng Xu1Chun-quan Cai2Tianjin Medical UniversityDepartment of Respiratory, Tianjin Children’s HospitalDepartment of Neurosurgery, Tianjin Institute of Pediatrics, The Children’s Hospital of TianjinAbstract Background Excessive fibroblast proliferation during pulmonary fibrosis leads to structural abnormalities in lung tissue and causes hypoxia and cell injury. However, the mechanisms and effective treatment are still limited. Methods In vivo, we used bleomycin to induce pulmonary fibrosis in mice. IHC and Masson staining were used to evaluate the inhibitory effect of ginsenoside Rg3 in pulmonary fibrosis. In vitro, scanning electron microscopy, transwell and wound healing were used to evaluate the cell phenotype of LL 29 cells. In addition, biacore was used to detect the binding between ginsenoside Rg3 and HIF-1α. Results Here, we found that bleomycin induces the activation of the HIF-1α/TGFβ1 signalling pathway and further enhances the migration and proliferation of fibroblasts through the epithelial mesenchymal transition (EMT). In addition, molecular docking and biacore results indicated that ginsenoside Rg3 can bind HIF-1α. Therefore, Ginsenoside Rg3 can slow down the progression of pulmonary fibrosis by inhibiting the nuclear localisation of HIF-1α. Conclusions This finding suggests that early targeted treatment of hypoxia may have potential value in the treatment of pulmonary fibrosis.https://doi.org/10.1186/s12890-021-01426-5GinsenosidePulmonary fibrosisHIF-1αEpithelial mesenchymal transition |
spellingShingle | Zhuo Fu Yong-sheng Xu Chun-quan Cai Ginsenoside Rg3 inhibits pulmonary fibrosis by preventing HIF-1α nuclear localisation BMC Pulmonary Medicine Ginsenoside Pulmonary fibrosis HIF-1α Epithelial mesenchymal transition |
title | Ginsenoside Rg3 inhibits pulmonary fibrosis by preventing HIF-1α nuclear localisation |
title_full | Ginsenoside Rg3 inhibits pulmonary fibrosis by preventing HIF-1α nuclear localisation |
title_fullStr | Ginsenoside Rg3 inhibits pulmonary fibrosis by preventing HIF-1α nuclear localisation |
title_full_unstemmed | Ginsenoside Rg3 inhibits pulmonary fibrosis by preventing HIF-1α nuclear localisation |
title_short | Ginsenoside Rg3 inhibits pulmonary fibrosis by preventing HIF-1α nuclear localisation |
title_sort | ginsenoside rg3 inhibits pulmonary fibrosis by preventing hif 1α nuclear localisation |
topic | Ginsenoside Pulmonary fibrosis HIF-1α Epithelial mesenchymal transition |
url | https://doi.org/10.1186/s12890-021-01426-5 |
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