Harnessing multiplex crRNA enables an amplification-free/CRISPR-Cas12a-based diagnostic methodology for Nosema bombycis

Harnessing multiplex crRNA enables an amplification-free/CRISPR-Cas12a-based diagnostic methodology for Nosema bombycis

ABSTRACT Nosema bombycis is transmitted horizontally and vertically from infected female moths to progeny eggs, bringing substantial economic losses to sericulture production. Timely and accurate detection of N. bombycis can effectively block the spread of silkworm pébrine disease, which is of great...

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Bibliographic Details
Main Authors: Huarui Zhang, Huijuan Zhao, Lu Cao, Bin Yu, Junhong Wei, Guoqing Pan, Jialing Bao, Zeyang Zhou
Format: Article
Language:English
Published: American Society for Microbiology 2024-01-01
Series:Microbiology Spectrum
Subjects:
Nosema bombycis
CRISPR/Cas12a
multiplex crRNA
amplification-free DNA diagnostic
pathogen detection
Online Access:https://journals.asm.org/doi/10.1128/spectrum.03014-23

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