Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic-ischemic encephalopathy
Abstract Background Hypoxic-ischemic encephalopathy (HIE) is a common disease that occurs during the perinatal period. The primary cause of neonatal HIE is related to fetal intrauterine anoxia. Carbamylated erythropoietin (CEPO), a derivative of erythropoietin (EPO), does not exert any erythropoieti...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Biological Research |
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| Online Access: | http://link.springer.com/article/10.1186/s40659-019-0234-7 |
| _version_ | 1819098765754105856 |
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| author | Min Diao Yi Qu Hui Liu Yushan Ma Xuemei Lin |
| author_facet | Min Diao Yi Qu Hui Liu Yushan Ma Xuemei Lin |
| author_sort | Min Diao |
| collection | DOAJ |
| description | Abstract Background Hypoxic-ischemic encephalopathy (HIE) is a common disease that occurs during the perinatal period. The primary cause of neonatal HIE is related to fetal intrauterine anoxia. Carbamylated erythropoietin (CEPO), a derivative of erythropoietin (EPO), does not exert any erythropoietic effect; however, the neuroprotective effects resemble those of EPO. Previous studies have shown the potential benefits of CEPO on the central nervous system. The present study aimed to investigate the role of CEPO in neuronal apoptosis during intrauterine HIE and the underlying mechanisms. Results To validate our hypothesis, we established an intrauterine HIE model by occluding the bilateral utero-ovarian arteries of pregnant Sprague–Dawley rats. Compared to the I/R group, neuronal apoptosis in the CEPO group was significantly lower at 4, 12, 24, and 48 h (P < 0.05). CEPO significantly inhibited CC3 expression (P < 0.05) during the early-stages after ischemia–reperfusion (0.5, 4, 8, 12 and 24 h), upregulated Bcl-2 expression, and downregulated Bax expression at 4, 8, 12, and 24 h (P < 0.05). Conclusions Carbamylated erythropoietin pretreatment inhibited the expression of proapoptotic protein CC3 in brain and regulated the Bcl-2/Bax ratio, resulting in reduced neuronal apoptosis and thus resulting in a protective effect on intrauterine HIE. |
| first_indexed | 2024-12-22T00:36:11Z |
| format | Article |
| id | doaj.art-4928baf6d5104cc8829feef5a8439b1a |
| institution | Directory Open Access Journal |
| issn | 0717-6287 |
| language | English |
| last_indexed | 2024-12-22T00:36:11Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-4928baf6d5104cc8829feef5a8439b1a2022-12-21T18:44:48ZengBMCBiological Research0717-62872019-05-0152111110.1186/s40659-019-0234-7Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic-ischemic encephalopathyMin Diao0Yi Qu1Hui Liu2Yushan Ma3Xuemei Lin4Department of Anesthesiology, West China Second University Hospital, Sichuan UniversityKey Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of EducationDepartment of Anesthesiology, West China Second University Hospital, Sichuan UniversityDepartment of Anesthesiology, West China Second University Hospital, Sichuan UniversityDepartment of Anesthesiology, West China Second University Hospital, Sichuan UniversityAbstract Background Hypoxic-ischemic encephalopathy (HIE) is a common disease that occurs during the perinatal period. The primary cause of neonatal HIE is related to fetal intrauterine anoxia. Carbamylated erythropoietin (CEPO), a derivative of erythropoietin (EPO), does not exert any erythropoietic effect; however, the neuroprotective effects resemble those of EPO. Previous studies have shown the potential benefits of CEPO on the central nervous system. The present study aimed to investigate the role of CEPO in neuronal apoptosis during intrauterine HIE and the underlying mechanisms. Results To validate our hypothesis, we established an intrauterine HIE model by occluding the bilateral utero-ovarian arteries of pregnant Sprague–Dawley rats. Compared to the I/R group, neuronal apoptosis in the CEPO group was significantly lower at 4, 12, 24, and 48 h (P < 0.05). CEPO significantly inhibited CC3 expression (P < 0.05) during the early-stages after ischemia–reperfusion (0.5, 4, 8, 12 and 24 h), upregulated Bcl-2 expression, and downregulated Bax expression at 4, 8, 12, and 24 h (P < 0.05). Conclusions Carbamylated erythropoietin pretreatment inhibited the expression of proapoptotic protein CC3 in brain and regulated the Bcl-2/Bax ratio, resulting in reduced neuronal apoptosis and thus resulting in a protective effect on intrauterine HIE.http://link.springer.com/article/10.1186/s40659-019-0234-7Carbamylated erythropoietinNeuronal apoptosisIntrauterine hypoxic-ischemic encephalopathy |
| spellingShingle | Min Diao Yi Qu Hui Liu Yushan Ma Xuemei Lin Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic-ischemic encephalopathy Biological Research Carbamylated erythropoietin Neuronal apoptosis Intrauterine hypoxic-ischemic encephalopathy |
| title | Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic-ischemic encephalopathy |
| title_full | Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic-ischemic encephalopathy |
| title_fullStr | Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic-ischemic encephalopathy |
| title_full_unstemmed | Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic-ischemic encephalopathy |
| title_short | Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic-ischemic encephalopathy |
| title_sort | effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic ischemic encephalopathy |
| topic | Carbamylated erythropoietin Neuronal apoptosis Intrauterine hypoxic-ischemic encephalopathy |
| url | http://link.springer.com/article/10.1186/s40659-019-0234-7 |
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