Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticus
Vibrio parahaemolyticus, a Gram-negative, halophilic bacterium, is a leading cause of acute gastroenteritis in humans. AphA and OpaR are the master quorum sensing (QS) regulators operating at low cell density (LCD) and high cell density (HCD), respectively. QsvR is an AraC-type protein that integrat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1123524/full |
| _version_ | 1797948170055974912 |
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| author | Yiquan Zhang Xingfan Xue Xingfan Xue Fengjun Sun Xue Li Miaomiao Zhang Miaomiao Zhang Qimin Wu Tingting Zhang Xi Luo Renfei Lu |
| author_facet | Yiquan Zhang Xingfan Xue Xingfan Xue Fengjun Sun Xue Li Miaomiao Zhang Miaomiao Zhang Qimin Wu Tingting Zhang Xi Luo Renfei Lu |
| author_sort | Yiquan Zhang |
| collection | DOAJ |
| description | Vibrio parahaemolyticus, a Gram-negative, halophilic bacterium, is a leading cause of acute gastroenteritis in humans. AphA and OpaR are the master quorum sensing (QS) regulators operating at low cell density (LCD) and high cell density (HCD), respectively. QsvR is an AraC-type protein that integrates into the QS system to control gene expression by directly controlling the transcription of aphA and opaR. However, the regulation of QsvR itself remains unclear to date. In this study, we show that vpa0607 and qsvR are transcribed as an operon, vpa0607-qsvR. AphA indirectly activates the transcription of vpa0607 at LCD, whereas OpaR and QsvR directly repress vpa0607 transcription at HCD, leading to the highest expression levels of vpa0607 occurs at LCD. Moreover, VPA0607 acts as an active RNase II-type protein in V. parahaemolyticus and feedback inhibits the expression of QsvR at the post-transcriptional level. Taken together, this work deepens our understanding of the regulation of QsvR and enriches the integration mechanisms of QsvR with the QS system in V. parahaemolyticus. |
| first_indexed | 2024-04-10T21:39:06Z |
| format | Article |
| id | doaj.art-4931b81e1834435cb2dde4520f8b1010 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T21:39:06Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4931b81e1834435cb2dde4520f8b10102023-01-19T06:53:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011410.3389/fmicb.2023.11235241123524Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticusYiquan Zhang0Xingfan Xue1Xingfan Xue2Fengjun Sun3Xue Li4Miaomiao Zhang5Miaomiao Zhang6Qimin Wu7Tingting Zhang8Xi Luo9Renfei Lu10Department of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, ChinaDepartment of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, ChinaSchool of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaDepartment of Pharmacy, The First Affiliated Hospital of Army Medical University, Chongqing, ChinaDepartment of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, ChinaDepartment of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, ChinaSchool of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaDepartment of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, ChinaDepartment of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, ChinaDepartment of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, ChinaDepartment of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, ChinaVibrio parahaemolyticus, a Gram-negative, halophilic bacterium, is a leading cause of acute gastroenteritis in humans. AphA and OpaR are the master quorum sensing (QS) regulators operating at low cell density (LCD) and high cell density (HCD), respectively. QsvR is an AraC-type protein that integrates into the QS system to control gene expression by directly controlling the transcription of aphA and opaR. However, the regulation of QsvR itself remains unclear to date. In this study, we show that vpa0607 and qsvR are transcribed as an operon, vpa0607-qsvR. AphA indirectly activates the transcription of vpa0607 at LCD, whereas OpaR and QsvR directly repress vpa0607 transcription at HCD, leading to the highest expression levels of vpa0607 occurs at LCD. Moreover, VPA0607 acts as an active RNase II-type protein in V. parahaemolyticus and feedback inhibits the expression of QsvR at the post-transcriptional level. Taken together, this work deepens our understanding of the regulation of QsvR and enriches the integration mechanisms of QsvR with the QS system in V. parahaemolyticus.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1123524/fullVibrio parahaemolyticusquorum sensingQsvRVPA0607RNase II |
| spellingShingle | Yiquan Zhang Xingfan Xue Xingfan Xue Fengjun Sun Xue Li Miaomiao Zhang Miaomiao Zhang Qimin Wu Tingting Zhang Xi Luo Renfei Lu Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticus Frontiers in Microbiology Vibrio parahaemolyticus quorum sensing QsvR VPA0607 RNase II |
| title | Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticus |
| title_full | Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticus |
| title_fullStr | Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticus |
| title_full_unstemmed | Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticus |
| title_short | Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticus |
| title_sort | quorum sensing and qsvr tightly control the transcription of vpa0607 encoding an active rnase ii type protein in vibrio parahaemolyticus |
| topic | Vibrio parahaemolyticus quorum sensing QsvR VPA0607 RNase II |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1123524/full |
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