A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus
Rabbit Viral Hemorrhagic Disease (RHD) is a highly contagious and fatal infection, resulting in considerable economic losses to the rabbit industry. Consequently, it is essential to develop a fast and accurate diagnostic method for RHDV GI.1. In this study, a rapid simple reverse transcriptase recom...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1056601/full |
| _version_ | 1811155482461601792 |
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| author | Lianzhi Zhang Qiaoya Zhao Ye Tian Yi Tang Yixin Wang Bing Huang |
| author_facet | Lianzhi Zhang Qiaoya Zhao Ye Tian Yi Tang Yixin Wang Bing Huang |
| author_sort | Lianzhi Zhang |
| collection | DOAJ |
| description | Rabbit Viral Hemorrhagic Disease (RHD) is a highly contagious and fatal infection, resulting in considerable economic losses to the rabbit industry. Consequently, it is essential to develop a fast and accurate diagnostic method for RHDV GI.1. In this study, a rapid simple reverse transcriptase recombinase polymerase amplification (RTRPA) for RHDV GI.1 was successfully developed using specific primers to RHDV GI.1 VP60 gene. Results indicated that the entire amplification process could be achieved in an isothermal condition at 40°C for 30 minutes, with good specificity and no reaction to other common rabbit disease pathogens, and a high sensitivity of upto 0.1LD50 of RHDV GI.1. Then, RT-RPA method was used to detect 1144 clinical samples, and the positive rates were 0.95%, 1.29% and 2.50% in Zaozhuang, Linyi, and Liaocheng in Shandong Province, respectively (the Fisher's exact test, P = 0.413), suggesting that there is no significant difference in RHDV GI.1 infection among the different regions. In conclusion, this study established a RT-RPA assay which is suitable for quick detection and monitoring of RHDV GI.1, thus making it a viable option for epidemiological surveillance. |
| first_indexed | 2024-04-10T04:33:44Z |
| format | Article |
| id | doaj.art-49320201a6ec4316a07ccbbf6e06cf8b |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T04:33:44Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-49320201a6ec4316a07ccbbf6e06cf8b2023-03-10T04:45:22ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-03-011010.3389/fvets.2023.10566011056601A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virusLianzhi Zhang0Qiaoya Zhao1Ye Tian2Yi Tang3Yixin Wang4Bing Huang5College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, ChinaInstitute of Poultry Sciences, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Poultry Sciences, Shandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, ChinaInstitute of Poultry Sciences, Shandong Academy of Agricultural Sciences, Jinan, ChinaRabbit Viral Hemorrhagic Disease (RHD) is a highly contagious and fatal infection, resulting in considerable economic losses to the rabbit industry. Consequently, it is essential to develop a fast and accurate diagnostic method for RHDV GI.1. In this study, a rapid simple reverse transcriptase recombinase polymerase amplification (RTRPA) for RHDV GI.1 was successfully developed using specific primers to RHDV GI.1 VP60 gene. Results indicated that the entire amplification process could be achieved in an isothermal condition at 40°C for 30 minutes, with good specificity and no reaction to other common rabbit disease pathogens, and a high sensitivity of upto 0.1LD50 of RHDV GI.1. Then, RT-RPA method was used to detect 1144 clinical samples, and the positive rates were 0.95%, 1.29% and 2.50% in Zaozhuang, Linyi, and Liaocheng in Shandong Province, respectively (the Fisher's exact test, P = 0.413), suggesting that there is no significant difference in RHDV GI.1 infection among the different regions. In conclusion, this study established a RT-RPA assay which is suitable for quick detection and monitoring of RHDV GI.1, thus making it a viable option for epidemiological surveillance.https://www.frontiersin.org/articles/10.3389/fvets.2023.1056601/fullrabbit hemorrhagic disease virusGI.1 genotypeisothermalrecombinase polymerase amplificationrapid detection methodepidemiological surveillance |
| spellingShingle | Lianzhi Zhang Qiaoya Zhao Ye Tian Yi Tang Yixin Wang Bing Huang A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus Frontiers in Veterinary Science rabbit hemorrhagic disease virus GI.1 genotype isothermal recombinase polymerase amplification rapid detection method epidemiological surveillance |
| title | A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus |
| title_full | A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus |
| title_fullStr | A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus |
| title_full_unstemmed | A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus |
| title_short | A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus |
| title_sort | novel reverse transcription recombinase polymerase amplification assay for rapid detection of gi 1 genotype of rabbit hemorrhagic disease virus |
| topic | rabbit hemorrhagic disease virus GI.1 genotype isothermal recombinase polymerase amplification rapid detection method epidemiological surveillance |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1056601/full |
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