The Carboxy Terminal Region on Spike Protein of Porcine Epidemic Diarrhea Virus (PEDV) Is Important for Evaluating Neutralizing Activity
In this study, we evaluated 62 sow sera samples from PED-vaccinated sows to compare the serum neutralizing test (SNT) and enzyme-linked immunosorbent assay (ELISA). We performed protein ELISA (pELISA) using fragments of spike proteins S1, S2, S3 and entire nucleocapsid proteins, and found a correlat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/6/683 |
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| author | Ki-Jong Kang Dong-Hwan Kim Eui-Ju Hong Hyun-Jin Shin |
| author_facet | Ki-Jong Kang Dong-Hwan Kim Eui-Ju Hong Hyun-Jin Shin |
| author_sort | Ki-Jong Kang |
| collection | DOAJ |
| description | In this study, we evaluated 62 sow sera samples from PED-vaccinated sows to compare the serum neutralizing test (SNT) and enzyme-linked immunosorbent assay (ELISA). We performed protein ELISA (pELISA) using fragments of spike proteins S1, S2, S3 and entire nucleocapsid proteins, and found a correlation between the SNT and ELISA in PEDV-vaccinated sera. Sera with higher neutralizing activity showed higher titers of IgG. In the antibody profiling, the neutralizing activities are correlated with the levels of the spike antibody, especially the S3 region. We confirmed that the carboxy-terminal region, including the endodomain of the S protein, induced stronger neutralizing activity than the ectodomain. This region of the S protein could be useful for evaluating PED vaccine efficacy, and it is a strong neutralizing epitope of PEDV. The S3 protein could be useful for evaluating PED vaccine efficacy, and it is a strong neutralizing epitope of PEDV. |
| first_indexed | 2024-03-10T10:50:41Z |
| format | Article |
| id | doaj.art-4935720875ed452183de1b9104bc5693 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T10:50:41Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-4935720875ed452183de1b9104bc56932023-11-21T22:14:05ZengMDPI AGPathogens2076-08172021-05-0110668310.3390/pathogens10060683The Carboxy Terminal Region on Spike Protein of Porcine Epidemic Diarrhea Virus (PEDV) Is Important for Evaluating Neutralizing ActivityKi-Jong Kang0Dong-Hwan Kim1Eui-Ju Hong2Hyun-Jin Shin3Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejon 34134, KoreaLaboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejon 34134, KoreaLaboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejon 34134, KoreaLaboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejon 34134, KoreaIn this study, we evaluated 62 sow sera samples from PED-vaccinated sows to compare the serum neutralizing test (SNT) and enzyme-linked immunosorbent assay (ELISA). We performed protein ELISA (pELISA) using fragments of spike proteins S1, S2, S3 and entire nucleocapsid proteins, and found a correlation between the SNT and ELISA in PEDV-vaccinated sera. Sera with higher neutralizing activity showed higher titers of IgG. In the antibody profiling, the neutralizing activities are correlated with the levels of the spike antibody, especially the S3 region. We confirmed that the carboxy-terminal region, including the endodomain of the S protein, induced stronger neutralizing activity than the ectodomain. This region of the S protein could be useful for evaluating PED vaccine efficacy, and it is a strong neutralizing epitope of PEDV. The S3 protein could be useful for evaluating PED vaccine efficacy, and it is a strong neutralizing epitope of PEDV.https://www.mdpi.com/2076-0817/10/6/683porcine epidemic diarrhea virusspike proteincarboxy terminalneutralizing activity |
| spellingShingle | Ki-Jong Kang Dong-Hwan Kim Eui-Ju Hong Hyun-Jin Shin The Carboxy Terminal Region on Spike Protein of Porcine Epidemic Diarrhea Virus (PEDV) Is Important for Evaluating Neutralizing Activity Pathogens porcine epidemic diarrhea virus spike protein carboxy terminal neutralizing activity |
| title | The Carboxy Terminal Region on Spike Protein of Porcine Epidemic Diarrhea Virus (PEDV) Is Important for Evaluating Neutralizing Activity |
| title_full | The Carboxy Terminal Region on Spike Protein of Porcine Epidemic Diarrhea Virus (PEDV) Is Important for Evaluating Neutralizing Activity |
| title_fullStr | The Carboxy Terminal Region on Spike Protein of Porcine Epidemic Diarrhea Virus (PEDV) Is Important for Evaluating Neutralizing Activity |
| title_full_unstemmed | The Carboxy Terminal Region on Spike Protein of Porcine Epidemic Diarrhea Virus (PEDV) Is Important for Evaluating Neutralizing Activity |
| title_short | The Carboxy Terminal Region on Spike Protein of Porcine Epidemic Diarrhea Virus (PEDV) Is Important for Evaluating Neutralizing Activity |
| title_sort | carboxy terminal region on spike protein of porcine epidemic diarrhea virus pedv is important for evaluating neutralizing activity |
| topic | porcine epidemic diarrhea virus spike protein carboxy terminal neutralizing activity |
| url | https://www.mdpi.com/2076-0817/10/6/683 |
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