Field diagnostic techniques to determine nitrogen in maize

Few technologies are available to quantify crop's nutritional status during their vegetative cycle, such as in maize. Semiquantitative alternatives could replace the laboratory foliar analysis's classical approach for measuring nitrogen (N) in maize (Zea mays L.) in the field, although the...

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Main Authors: Eduhin Roacho-Cortés, Javier Z. Castellanos-Ramos, J. D. Etchevers
Format: Article
Language:English
Published: Sociedad Mexicana de la Ciencia del Suelo A. C. 2021-04-01
Series:Terra Latinoamericana
Subjects:
Online Access:https://www.terralatinoamericana.org.mx/index.php/terra/article/view/820
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author Eduhin Roacho-Cortés
Javier Z. Castellanos-Ramos
J. D. Etchevers
author_facet Eduhin Roacho-Cortés
Javier Z. Castellanos-Ramos
J. D. Etchevers
author_sort Eduhin Roacho-Cortés
collection DOAJ
description Few technologies are available to quantify crop's nutritional status during their vegetative cycle, such as in maize. Semiquantitative alternatives could replace the laboratory foliar analysis's classical approach for measuring nitrogen (N) in maize (Zea mays L.) in the field, although they require regional calibration against standard laboratory tests. In this work, we have calibrated four semi quantitative and two quantitative methods for diagnosing maize nutritional status in the El Bajio region, Guanajuato, Mexico. An experiment to test the effect of increasing N rates application on plant sap (cell extract) N was set up during the 2019 agricultural cycle. Five N treatments (0, 120, 240, 360, and 480 kg of N·ha-1) were tested, and each treatment was replicated five times. We have tested the following semi quantitative N methods: plant sap nitrate concentration (N-NO3) of the central midrib of the recently mature leaf (RML), plant sap N-NO3 of the base stem (in both cases measured by the ion selective method, using the portable LAQUAtwin® model NO3-11 nitrate ion meter, single-photon avalanche diode (SPAD) unit record, and the Normalized Difference Vegetation Index (NDVI). Additionally, a quantitative laboratory method for determining N-NO3 in the lower part of the maize plant's stem and total N in the RML was used. Data were acquired at different plant phenological stages: V7, V12, R1, and N-NO3 in the dry matter of the stem's base at R5. These results were correlated with the relative grain yield and those with a correlation coefficient (r) greater than 0.7 were accepted. Later, a critical concentration (CC) was estimated using an arithmetic-graphic technique for the crop to reach a relative yield of 90% for each method. The proposed CC results require further validation before being used in a commercial application. In synthesis, this work proposes a procedure for diagnosing N nutrition of maize in the field that might improve the current methods used for this purpose.
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spelling doaj.art-493a929de526482d9318307bc5975d0f2022-12-21T21:05:14ZengSociedad Mexicana de la Ciencia del Suelo A. C.Terra Latinoamericana0187-57792395-80302021-04-01390112https://doi.org/10.28940/terra.v39i0.820Field diagnostic techniques to determine nitrogen in maizeEduhin Roacho-Cortés0https://orcid.org/0000-0002-2860-1460Javier Z. Castellanos-Ramos1https://orcid.org/0000-0003-3886-6574J. D. Etchevers2https://orcid.org/0000-0003-4663-6306Colegio de Postgraduados, Campus Montecillo. Carretera México-Texcoco km 36.5, Montecillo. 56230 Texcoco, Estado de México, México.Fertilab. Poniente 6 #200, Cd. Industrial de Celaya. 38010 Celaya, Guanajuato, México.Colegio de Postgraduados, Campus Montecillo. Carretera México-Texcoco km 36.5, Montecillo. 56230 Texcoco, Estado de México, México.Few technologies are available to quantify crop's nutritional status during their vegetative cycle, such as in maize. Semiquantitative alternatives could replace the laboratory foliar analysis's classical approach for measuring nitrogen (N) in maize (Zea mays L.) in the field, although they require regional calibration against standard laboratory tests. In this work, we have calibrated four semi quantitative and two quantitative methods for diagnosing maize nutritional status in the El Bajio region, Guanajuato, Mexico. An experiment to test the effect of increasing N rates application on plant sap (cell extract) N was set up during the 2019 agricultural cycle. Five N treatments (0, 120, 240, 360, and 480 kg of N·ha-1) were tested, and each treatment was replicated five times. We have tested the following semi quantitative N methods: plant sap nitrate concentration (N-NO3) of the central midrib of the recently mature leaf (RML), plant sap N-NO3 of the base stem (in both cases measured by the ion selective method, using the portable LAQUAtwin® model NO3-11 nitrate ion meter, single-photon avalanche diode (SPAD) unit record, and the Normalized Difference Vegetation Index (NDVI). Additionally, a quantitative laboratory method for determining N-NO3 in the lower part of the maize plant's stem and total N in the RML was used. Data were acquired at different plant phenological stages: V7, V12, R1, and N-NO3 in the dry matter of the stem's base at R5. These results were correlated with the relative grain yield and those with a correlation coefficient (r) greater than 0.7 were accepted. Later, a critical concentration (CC) was estimated using an arithmetic-graphic technique for the crop to reach a relative yield of 90% for each method. The proposed CC results require further validation before being used in a commercial application. In synthesis, this work proposes a procedure for diagnosing N nutrition of maize in the field that might improve the current methods used for this purpose.https://www.terralatinoamericana.org.mx/index.php/terra/article/view/820ion selective methodnitrate-nitrogenndviplant sapspadtotal nitrogenzea mays
spellingShingle Eduhin Roacho-Cortés
Javier Z. Castellanos-Ramos
J. D. Etchevers
Field diagnostic techniques to determine nitrogen in maize
Terra Latinoamericana
ion selective method
nitrate-nitrogen
ndvi
plant sap
spad
total nitrogen
zea mays
title Field diagnostic techniques to determine nitrogen in maize
title_full Field diagnostic techniques to determine nitrogen in maize
title_fullStr Field diagnostic techniques to determine nitrogen in maize
title_full_unstemmed Field diagnostic techniques to determine nitrogen in maize
title_short Field diagnostic techniques to determine nitrogen in maize
title_sort field diagnostic techniques to determine nitrogen in maize
topic ion selective method
nitrate-nitrogen
ndvi
plant sap
spad
total nitrogen
zea mays
url https://www.terralatinoamericana.org.mx/index.php/terra/article/view/820
work_keys_str_mv AT eduhinroachocortes fielddiagnostictechniquestodeterminenitrogeninmaize
AT javierzcastellanosramos fielddiagnostictechniquestodeterminenitrogeninmaize
AT jdetchevers fielddiagnostictechniquestodeterminenitrogeninmaize