A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance
Nanoporous SnO2 thin films were elaborated to serve as sensing electrodes for label-free DNA detection using electrochemical impedance spectroscopy (EIS). Films were deposited by an electrodeposition process (EDP). Then the non-Faradic EIS behaviour was thoroughly investigated during some different...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-05-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/15/5/10686 |
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| author | Minh Hai Le Carmen Jimenez Eric Chainet Valerie Stambouli |
| author_facet | Minh Hai Le Carmen Jimenez Eric Chainet Valerie Stambouli |
| author_sort | Minh Hai Le |
| collection | DOAJ |
| description | Nanoporous SnO2 thin films were elaborated to serve as sensing electrodes for label-free DNA detection using electrochemical impedance spectroscopy (EIS). Films were deposited by an electrodeposition process (EDP). Then the non-Faradic EIS behaviour was thoroughly investigated during some different steps of functionalization up to DNA hybridization. The results have shown a systematic decrease of the impedance upon DNA hybridization. The impedance decrease is attributed to an enhanced penetration of ionic species within the film volume. Besides, the comparison of impedance variations upon DNA hybridization between the liquid and vapour phase processes for organosilane (APTES) grafting on the nanoporous SnO2 films showed that vapour-phase method is more efficient. This is due to the fact that the vapour is more effective than the solution in penetrating the nanopores of the films. As a result, the DNA sensors built from vapour-treated silane layer exhibit a higher sensitivity than those produced from liquid-treated silane, in the range of tested target DNA concentration going to 10 nM. Finally, the impedance and fluorescence response signals strongly depend on the types of target DNA molecules, demonstrating a high selectivity of the process on nanoporous SnO2 films. |
| first_indexed | 2024-04-12T19:46:25Z |
| format | Article |
| id | doaj.art-493cee419f024bf69be1f510a9a1f621 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-12T19:46:25Z |
| publishDate | 2015-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-493cee419f024bf69be1f510a9a1f6212022-12-22T03:18:57ZengMDPI AGSensors1424-82202015-05-01155106861070410.3390/s150510686s150510686A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection PerformanceMinh Hai Le0Carmen Jimenez1Eric Chainet2Valerie Stambouli3Université Grenoble-Alpes, CNRS, Laboratoire des Matériaux et du Génie Physique (LMGP), MINATEC, 3 parvis Louis Néel, 38016 Grenoble Cedex 1, FranceUniversité Grenoble-Alpes, CNRS, Laboratoire des Matériaux et du Génie Physique (LMGP), MINATEC, 3 parvis Louis Néel, 38016 Grenoble Cedex 1, FranceLaboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI), 1130 rue de la Piscine BP75, 38402 Saint Martin d'Hères Cedex, FranceUniversité Grenoble-Alpes, CNRS, Laboratoire des Matériaux et du Génie Physique (LMGP), MINATEC, 3 parvis Louis Néel, 38016 Grenoble Cedex 1, FranceNanoporous SnO2 thin films were elaborated to serve as sensing electrodes for label-free DNA detection using electrochemical impedance spectroscopy (EIS). Films were deposited by an electrodeposition process (EDP). Then the non-Faradic EIS behaviour was thoroughly investigated during some different steps of functionalization up to DNA hybridization. The results have shown a systematic decrease of the impedance upon DNA hybridization. The impedance decrease is attributed to an enhanced penetration of ionic species within the film volume. Besides, the comparison of impedance variations upon DNA hybridization between the liquid and vapour phase processes for organosilane (APTES) grafting on the nanoporous SnO2 films showed that vapour-phase method is more efficient. This is due to the fact that the vapour is more effective than the solution in penetrating the nanopores of the films. As a result, the DNA sensors built from vapour-treated silane layer exhibit a higher sensitivity than those produced from liquid-treated silane, in the range of tested target DNA concentration going to 10 nM. Finally, the impedance and fluorescence response signals strongly depend on the types of target DNA molecules, demonstrating a high selectivity of the process on nanoporous SnO2 films.http://www.mdpi.com/1424-8220/15/5/10686biosensorDNAlabel free detection, fluorescenceimpedance spectroscopynanoporous SnO2 films |
| spellingShingle | Minh Hai Le Carmen Jimenez Eric Chainet Valerie Stambouli A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance Sensors biosensor DNA label free detection, fluorescence impedance spectroscopy nanoporous SnO2 films |
| title | A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance |
| title_full | A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance |
| title_fullStr | A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance |
| title_full_unstemmed | A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance |
| title_short | A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance |
| title_sort | label free impedimetric dna sensor based on a nanoporous sno2 film fabrication and detection performance |
| topic | biosensor DNA label free detection, fluorescence impedance spectroscopy nanoporous SnO2 films |
| url | http://www.mdpi.com/1424-8220/15/5/10686 |
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