Modulation of Immunological, Biochemical, and Histopathological Changes of Airway Remodeling by Withania somnifera in an Experimental Model of Allergic Asthma in Rats
Objectives: Airway remodeling in asthma involves chronic inflammation associated with structural changes, which result in severe airflow limitation and very few therapeutic options. Thus, the present study was designed to experimentally evaluate the ameliorative effects of Withania somnifera (WS) ro...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2023-06-01
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| Series: | Journal of Pharmacopuncture |
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| Online Access: | http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2023.26.2.158 |
| _version_ | 1827912979188809728 |
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| author | Nafaa Hasan Ali Sana Rehman Maaz Naqvi Kavita Gulati Arunabha Ray |
| author_facet | Nafaa Hasan Ali Sana Rehman Maaz Naqvi Kavita Gulati Arunabha Ray |
| author_sort | Nafaa Hasan Ali |
| collection | DOAJ |
| description | Objectives: Airway remodeling in asthma involves chronic inflammation associated with structural changes, which result in severe airflow limitation and very few therapeutic options. Thus, the present study was designed to experimentally evaluate the ameliorative effects of Withania somnifera (WS) root extract against Ovalbumin (OVA)-induced airway remodeling in a rat model of asthma.Methods: Wistar rats were immunized (i.p) and challenged (aerosol) with ovalbumin (OVA), and the effects of WS extract were investigated on the development and progress of airway remodeling by assessing immunological, biochemical, and histological changes in these rats.Results: OVA-immunization and challenge in rats resulted in significant increases in the levels of IL-13, 8-OhdG, TGF-β, hydroxyproline, and periostin in bronchoalveolar lavage fluid (BALF) and serum/lung homogenate compared to normal control (saline only) rats, and these changes were attenuated after WS extract (200 and 400 mg/kg), as well as dexamethasone (DEX, 1 mg/kg) pretreatments. Further, WS extract attenuated histopathological changes and maintained lung integrity. In herb-drug interactions, sub-threshold doses of WS extract and DEX showed synergistic effects on all parameters studied as compared to either form of monotherapy.Conclusion: These results indicated that WS exerted significant protective effects against airway remodeling in the experimental model by modulating inflammatory and fibrotic cytokines, and could have the potential for developing a therapeutic alternative/adjunct for the treatment of airway remodeling of bronchial asthma. |
| first_indexed | 2024-03-13T02:23:57Z |
| format | Article |
| id | doaj.art-493d0e1fd612427185c6de140a450bff |
| institution | Directory Open Access Journal |
| issn | 2093-6966 |
| language | English |
| last_indexed | 2024-03-13T02:23:57Z |
| publishDate | 2023-06-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-493d0e1fd612427185c6de140a450bff2023-06-30T06:35:46ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662023-06-0126215816610.3831/KPI.2023.26.2.158KPI.2023.26.2.158Modulation of Immunological, Biochemical, and Histopathological Changes of Airway Remodeling by Withania somnifera in an Experimental Model of Allergic Asthma in RatsNafaa Hasan Ali0Sana Rehman1Maaz Naqvi2Kavita Gulati3Arunabha Ray4Department of Pharmacology, Hamdard Institute of Medical Sciences and Research (HIMSR), Jamia Hamdard University, New Delhi, IndiaDepartment of Pharmacology, Hamdard Institute of Medical Sciences and Research (HIMSR), Jamia Hamdard University, New Delhi, IndiaDepartment of Pharmacology, Hamdard Institute of Medical Sciences and Research (HIMSR), Jamia Hamdard University, New Delhi, IndiaDepartment of Pharmacology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, IndiaDepartment of Pharmacology, Hamdard Institute of Medical Sciences and Research (HIMSR), Jamia Hamdard University, New Delhi, IndiaObjectives: Airway remodeling in asthma involves chronic inflammation associated with structural changes, which result in severe airflow limitation and very few therapeutic options. Thus, the present study was designed to experimentally evaluate the ameliorative effects of Withania somnifera (WS) root extract against Ovalbumin (OVA)-induced airway remodeling in a rat model of asthma.Methods: Wistar rats were immunized (i.p) and challenged (aerosol) with ovalbumin (OVA), and the effects of WS extract were investigated on the development and progress of airway remodeling by assessing immunological, biochemical, and histological changes in these rats.Results: OVA-immunization and challenge in rats resulted in significant increases in the levels of IL-13, 8-OhdG, TGF-β, hydroxyproline, and periostin in bronchoalveolar lavage fluid (BALF) and serum/lung homogenate compared to normal control (saline only) rats, and these changes were attenuated after WS extract (200 and 400 mg/kg), as well as dexamethasone (DEX, 1 mg/kg) pretreatments. Further, WS extract attenuated histopathological changes and maintained lung integrity. In herb-drug interactions, sub-threshold doses of WS extract and DEX showed synergistic effects on all parameters studied as compared to either form of monotherapy.Conclusion: These results indicated that WS exerted significant protective effects against airway remodeling in the experimental model by modulating inflammatory and fibrotic cytokines, and could have the potential for developing a therapeutic alternative/adjunct for the treatment of airway remodeling of bronchial asthma.http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2023.26.2.158bronchial asthmaairway remodelingwithania somniferacytokinesoxidative stress |
| spellingShingle | Nafaa Hasan Ali Sana Rehman Maaz Naqvi Kavita Gulati Arunabha Ray Modulation of Immunological, Biochemical, and Histopathological Changes of Airway Remodeling by Withania somnifera in an Experimental Model of Allergic Asthma in Rats Journal of Pharmacopuncture bronchial asthma airway remodeling withania somnifera cytokines oxidative stress |
| title | Modulation of Immunological, Biochemical, and Histopathological Changes of Airway Remodeling by Withania somnifera in an Experimental Model of Allergic Asthma in Rats |
| title_full | Modulation of Immunological, Biochemical, and Histopathological Changes of Airway Remodeling by Withania somnifera in an Experimental Model of Allergic Asthma in Rats |
| title_fullStr | Modulation of Immunological, Biochemical, and Histopathological Changes of Airway Remodeling by Withania somnifera in an Experimental Model of Allergic Asthma in Rats |
| title_full_unstemmed | Modulation of Immunological, Biochemical, and Histopathological Changes of Airway Remodeling by Withania somnifera in an Experimental Model of Allergic Asthma in Rats |
| title_short | Modulation of Immunological, Biochemical, and Histopathological Changes of Airway Remodeling by Withania somnifera in an Experimental Model of Allergic Asthma in Rats |
| title_sort | modulation of immunological biochemical and histopathological changes of airway remodeling by withania somnifera in an experimental model of allergic asthma in rats |
| topic | bronchial asthma airway remodeling withania somnifera cytokines oxidative stress |
| url | http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2023.26.2.158 |
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