Unraveling the Impact of <i>Dab1</i> Gene Silencing on the Expression of Autophagy Markers in Lung Development
The purpose of this study was to evaluate the effects of <i>Dab1</i> gene silencing on the immunoexpression of light chain 3 beta (Lc3b), glucose regulating protein 78 (Grp78), heat shock cognate 71 (Hsc70), mammalian target of rapamycin (mTOR) and lysosomal-associated membrane protein 2...
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MDPI AG
2024-02-01
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| author | Azer Rizikalo Mirko Maglica Nela Kelam Ilija Perutina Marin Ogorevc Anita Racetin Natalija Filipović Yu Katsuyama Zdenka Zovko Josip Mišković Katarina Vukojević |
| author_facet | Azer Rizikalo Mirko Maglica Nela Kelam Ilija Perutina Marin Ogorevc Anita Racetin Natalija Filipović Yu Katsuyama Zdenka Zovko Josip Mišković Katarina Vukojević |
| author_sort | Azer Rizikalo |
| collection | DOAJ |
| description | The purpose of this study was to evaluate the effects of <i>Dab1</i> gene silencing on the immunoexpression of light chain 3 beta (Lc3b), glucose regulating protein 78 (Grp78), heat shock cognate 71 (Hsc70), mammalian target of rapamycin (mTOR) and lysosomal-associated membrane protein 2A (Lamp2a) in the lung tissue of developing <i>yotari</i> (<i>Dab1</i><sup>−/−</sup>) and wild-type (wt) mice. The lung epithelium and mesenchyme of the embryos at gestational days E13.5 and E15.5 were examined using immunofluorescence and semi-quantitative methods. In the pulmonary mesenchyme and epithelium, Grp78 and Lc3b of moderate fluorescence reactivity was demonstrated in wt mice for both evaluated time points, while <i>yotari</i> mice exhibited only epithelial reactivity for the same markers. Mild punctate expression of Hsc70 was observed for both genotypes. A significant difference was present when analyzing mTOR expression, where wt mice showed strong perinuclear staining in the epithelium. According to our data, <i>Dab1</i> gene silencing may result in autophagy abnormalities, which could then cause respiratory system pathologies via defective lung cell degradation by lysosome-dependent cell elimination. |
| first_indexed | 2024-04-24T18:04:37Z |
| format | Article |
| id | doaj.art-493ebfa43cae483a88ea407d9434f76b |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-04-24T18:04:37Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-493ebfa43cae483a88ea407d9434f76b2024-03-27T13:51:07ZengMDPI AGLife2075-17292024-02-0114331610.3390/life14030316Unraveling the Impact of <i>Dab1</i> Gene Silencing on the Expression of Autophagy Markers in Lung DevelopmentAzer Rizikalo0Mirko Maglica1Nela Kelam2Ilija Perutina3Marin Ogorevc4Anita Racetin5Natalija Filipović6Yu Katsuyama7Zdenka Zovko8Josip Mišković9Katarina Vukojević10Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and HerzegovinaDepartment of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and HerzegovinaDepartment of Anatomy, Histology and Embryology, University of Split School of Medicine, 21000 Split, CroatiaDepartment of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and HerzegovinaDepartment of Anatomy, Histology and Embryology, University of Split School of Medicine, 21000 Split, CroatiaDepartment of Anatomy, Histology and Embryology, University of Split School of Medicine, 21000 Split, CroatiaDepartment of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and HerzegovinaDepartment of Anatomy, Shiga University of Medical Science, Otsu 520-2192, JapanDepartment of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and HerzegovinaDepartment of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and HerzegovinaDepartment of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and HerzegovinaThe purpose of this study was to evaluate the effects of <i>Dab1</i> gene silencing on the immunoexpression of light chain 3 beta (Lc3b), glucose regulating protein 78 (Grp78), heat shock cognate 71 (Hsc70), mammalian target of rapamycin (mTOR) and lysosomal-associated membrane protein 2A (Lamp2a) in the lung tissue of developing <i>yotari</i> (<i>Dab1</i><sup>−/−</sup>) and wild-type (wt) mice. The lung epithelium and mesenchyme of the embryos at gestational days E13.5 and E15.5 were examined using immunofluorescence and semi-quantitative methods. In the pulmonary mesenchyme and epithelium, Grp78 and Lc3b of moderate fluorescence reactivity was demonstrated in wt mice for both evaluated time points, while <i>yotari</i> mice exhibited only epithelial reactivity for the same markers. Mild punctate expression of Hsc70 was observed for both genotypes. A significant difference was present when analyzing mTOR expression, where wt mice showed strong perinuclear staining in the epithelium. According to our data, <i>Dab1</i> gene silencing may result in autophagy abnormalities, which could then cause respiratory system pathologies via defective lung cell degradation by lysosome-dependent cell elimination.https://www.mdpi.com/2075-1729/14/3/316Lc3bGrp78Hsc70mTORLamp2a<i>yotari</i> |
| spellingShingle | Azer Rizikalo Mirko Maglica Nela Kelam Ilija Perutina Marin Ogorevc Anita Racetin Natalija Filipović Yu Katsuyama Zdenka Zovko Josip Mišković Katarina Vukojević Unraveling the Impact of <i>Dab1</i> Gene Silencing on the Expression of Autophagy Markers in Lung Development Life Lc3b Grp78 Hsc70 mTOR Lamp2a <i>yotari</i> |
| title | Unraveling the Impact of <i>Dab1</i> Gene Silencing on the Expression of Autophagy Markers in Lung Development |
| title_full | Unraveling the Impact of <i>Dab1</i> Gene Silencing on the Expression of Autophagy Markers in Lung Development |
| title_fullStr | Unraveling the Impact of <i>Dab1</i> Gene Silencing on the Expression of Autophagy Markers in Lung Development |
| title_full_unstemmed | Unraveling the Impact of <i>Dab1</i> Gene Silencing on the Expression of Autophagy Markers in Lung Development |
| title_short | Unraveling the Impact of <i>Dab1</i> Gene Silencing on the Expression of Autophagy Markers in Lung Development |
| title_sort | unraveling the impact of i dab1 i gene silencing on the expression of autophagy markers in lung development |
| topic | Lc3b Grp78 Hsc70 mTOR Lamp2a <i>yotari</i> |
| url | https://www.mdpi.com/2075-1729/14/3/316 |
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