Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay
Analysis of the fatty acid composition of total cellular lipids is important chemotaxonomic characteristic that is used to identify microorganisms, in particular actinobacteria, with the help of fatty acid spectra libraries. Actinobacteria are well known as producers of secondary metabolites, which...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2023-02-01
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| Series: | The Ukrainian Biochemical Journal |
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| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2023/04/Korotaeva_95_1.pdf |
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| author | N. V. Korotaeva K. S. Potapenko Z. Yu. Sergieieva N. Yu. Vasylieva V. O. Ivanytsia |
| author_facet | N. V. Korotaeva K. S. Potapenko Z. Yu. Sergieieva N. Yu. Vasylieva V. O. Ivanytsia |
| author_sort | N. V. Korotaeva |
| collection | DOAJ |
| description | Analysis of the fatty acid composition of total cellular lipids is important chemotaxonomic characteristic that is used to identify microorganisms, in particular actinobacteria, with the help of fatty acid spectra libraries. Actinobacteria are well known as producers of secondary metabolites, which are of pharmacological and commercial interest. A significant number of actinomycetes is associated with various marine benthic communities. The aim of this study was determination of the fatty acid composition and preliminary identification of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay. Actinobacteria of 12 isolated strains were grown in a liquid medium at 28° C for 72 h. Methyl esters of fatty acids were determined on a gas chromatograph Agilent. The MIDI Sherlock microorganisms identification system was used to identify the studied strains. It was found that the fatty acids methyl esters profiles of studied actinobacteria strains were characterized by the predominance of 12-17 saturated branched-chain fatty acids with high content of 12-methyltridecanoic, 12-methyltetradecanoic, 14-methylpentadecanoic and 14-methylhexadecanoic fatty acids. All 12 actinobacteria strains isolated from mussels (Mytilus galloprovincialis) of Odesa Bay were identified as members of the Streptomyces genus. |
| first_indexed | 2024-03-11T18:11:04Z |
| format | Article |
| id | doaj.art-49409d858df44c0eb3c54582f7519f50 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T18:11:04Z |
| publishDate | 2023-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-49409d858df44c0eb3c54582f7519f502023-10-16T12:21:57ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032023-02-01951858910.15407/ubj95.01.085Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa BayN. V. Korotaeva 0K. S. Potapenko1Z. Yu. Sergieieva2N. Yu. Vasylieva3V. O. Ivanytsia4Odesa National I. I. Mechnykov University, Odesa, UkraineOdesa National I. I. Mechnykov University, Odesa, UkraineOdesa National I. I. Mechnykov University, Odesa, UkraineOdesa National I. I. Mechnykov University, Odesa, UkraineOdesa National I. I. Mechnykov University, Odesa, UkraineAnalysis of the fatty acid composition of total cellular lipids is important chemotaxonomic characteristic that is used to identify microorganisms, in particular actinobacteria, with the help of fatty acid spectra libraries. Actinobacteria are well known as producers of secondary metabolites, which are of pharmacological and commercial interest. A significant number of actinomycetes is associated with various marine benthic communities. The aim of this study was determination of the fatty acid composition and preliminary identification of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay. Actinobacteria of 12 isolated strains were grown in a liquid medium at 28° C for 72 h. Methyl esters of fatty acids were determined on a gas chromatograph Agilent. The MIDI Sherlock microorganisms identification system was used to identify the studied strains. It was found that the fatty acids methyl esters profiles of studied actinobacteria strains were characterized by the predominance of 12-17 saturated branched-chain fatty acids with high content of 12-methyltridecanoic, 12-methyltetradecanoic, 14-methylpentadecanoic and 14-methylhexadecanoic fatty acids. All 12 actinobacteria strains isolated from mussels (Mytilus galloprovincialis) of Odesa Bay were identified as members of the Streptomyces genus.http://ukrbiochemjournal.org/wp-content/uploads/2023/04/Korotaeva_95_1.pdfblack seafatty acid compositionsmarine actinobacteriamytilus galloprovincialisstreptomyces |
| spellingShingle | N. V. Korotaeva K. S. Potapenko Z. Yu. Sergieieva N. Yu. Vasylieva V. O. Ivanytsia Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay The Ukrainian Biochemical Journal black sea fatty acid compositions marine actinobacteria mytilus galloprovincialis streptomyces |
| title | Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay |
| title_full | Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay |
| title_fullStr | Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay |
| title_full_unstemmed | Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay |
| title_short | Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay |
| title_sort | fatty acids composition of actinobacteria isolated from mussels mytilus galloprovincialis of the black sea odesa bay |
| topic | black sea fatty acid compositions marine actinobacteria mytilus galloprovincialis streptomyces |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2023/04/Korotaeva_95_1.pdf |
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