Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR
This study aimed to determine the prevalence of three common hemoparasites (Anaplasma marginale, Babesia bovis and Babesia bigemina) in cattle from 16 counties in the Campos de Lages region, Santa Catarina state, Brazil, and the factors affecting disease occurrence. The study population consisted of...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-08-01
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| Series: | Parasite Epidemiology and Control |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405673119300777 |
| _version_ | 1818967516853043200 |
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| author | Luisa Lemos Vieira Mariana Feltrin Canever Leonardo Leite Cardozo Cristina Perito Cardoso Marcos Edgar Herkenhoff André Thaler Neto Carla Ivane Ganz Vogel Luiz Claudio Miletti |
| author_facet | Luisa Lemos Vieira Mariana Feltrin Canever Leonardo Leite Cardozo Cristina Perito Cardoso Marcos Edgar Herkenhoff André Thaler Neto Carla Ivane Ganz Vogel Luiz Claudio Miletti |
| author_sort | Luisa Lemos Vieira |
| collection | DOAJ |
| description | This study aimed to determine the prevalence of three common hemoparasites (Anaplasma marginale, Babesia bovis and Babesia bigemina) in cattle from 16 counties in the Campos de Lages region, Santa Catarina state, Brazil, and the factors affecting disease occurrence. The study population consisted of 257 clinically healthy animals from 21 rural farms. Bovine blood samples were collected by jugular venipuncture. DNA was extracted from whole blood by the phenol/ chloroform method. Genomic DNA extracted from blood samples was subjected to Multiplex PCR for screening of B. bovis, B. bigemina, and A. marginale using specific primers. Prevalences of A. marginale, B. bigemina, and B. bovis were 27%, 16%, and 29%, respectively. Mixed infection was observed in 17.5% of samples. The most frequent was Babesia bovis and Babesia bigemina in 6.62% of samples. A. marginale infection rates were statistically correlated with age groups of cattle. The infections detected in the study population were considered to be subclinical, based on the presence pathogen DNA and absence of clinical symptoms. Seasonality of the pathogens resulted in various degrees of infection, related to the age of the animals and the season. The Campos de Lages region is characterized by enzootic instability for these pathogens because of its climatic and geographic features. Keywords: Multiplex PCR, Tick-borne disease, Brazil, Enzootic instability |
| first_indexed | 2024-12-20T13:50:03Z |
| format | Article |
| id | doaj.art-49435bbfbabd41349b3a5c08f084c0a5 |
| institution | Directory Open Access Journal |
| issn | 2405-6731 |
| language | English |
| last_indexed | 2024-12-20T13:50:03Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Parasite Epidemiology and Control |
| spelling | doaj.art-49435bbfbabd41349b3a5c08f084c0a52022-12-21T19:38:33ZengElsevierParasite Epidemiology and Control2405-67312019-08-016Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCRLuisa Lemos Vieira0Mariana Feltrin Canever1Leonardo Leite Cardozo2Cristina Perito Cardoso3Marcos Edgar Herkenhoff4André Thaler Neto5Carla Ivane Ganz Vogel6Luiz Claudio Miletti7Laboratório de Bioquímica de Hemoparasitas e Vetores (LABHEV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, n° 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, BrazilLaboratório de Bioquímica de Hemoparasitas e Vetores (LABHEV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, n° 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, BrazilSetor de Bovinocultura de Leite, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, n° 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, BrazilCompanhia Integrada de Desenvolvimento Agrícola de Santa Catarina, Rua João José Godinho, s/n° Guadalupe, 88506-080 Lages, SC, BrazilPrograma de Pós-graduação em Ciência Animal, Universidade do Estado de Santa Catarina, BrazilSetor de Bovinocultura de Leite, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, n° 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, BrazilLaboratório de Bioquímica de Hemoparasitas e Vetores (LABHEV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, n° 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, BrazilLaboratório de Bioquímica de Hemoparasitas e Vetores (LABHEV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, n° 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, Brazil; Corresponding author at: Departamento de Produção Animal e Alimentos, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Bairro Conta Dinheiro, 88520-000 Lages, SC, Brazil.This study aimed to determine the prevalence of three common hemoparasites (Anaplasma marginale, Babesia bovis and Babesia bigemina) in cattle from 16 counties in the Campos de Lages region, Santa Catarina state, Brazil, and the factors affecting disease occurrence. The study population consisted of 257 clinically healthy animals from 21 rural farms. Bovine blood samples were collected by jugular venipuncture. DNA was extracted from whole blood by the phenol/ chloroform method. Genomic DNA extracted from blood samples was subjected to Multiplex PCR for screening of B. bovis, B. bigemina, and A. marginale using specific primers. Prevalences of A. marginale, B. bigemina, and B. bovis were 27%, 16%, and 29%, respectively. Mixed infection was observed in 17.5% of samples. The most frequent was Babesia bovis and Babesia bigemina in 6.62% of samples. A. marginale infection rates were statistically correlated with age groups of cattle. The infections detected in the study population were considered to be subclinical, based on the presence pathogen DNA and absence of clinical symptoms. Seasonality of the pathogens resulted in various degrees of infection, related to the age of the animals and the season. The Campos de Lages region is characterized by enzootic instability for these pathogens because of its climatic and geographic features. Keywords: Multiplex PCR, Tick-borne disease, Brazil, Enzootic instabilityhttp://www.sciencedirect.com/science/article/pii/S2405673119300777 |
| spellingShingle | Luisa Lemos Vieira Mariana Feltrin Canever Leonardo Leite Cardozo Cristina Perito Cardoso Marcos Edgar Herkenhoff André Thaler Neto Carla Ivane Ganz Vogel Luiz Claudio Miletti Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR Parasite Epidemiology and Control |
| title | Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR |
| title_full | Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR |
| title_fullStr | Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR |
| title_full_unstemmed | Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR |
| title_short | Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR |
| title_sort | prevalence of anaplasma marginale babesia bovis and babesia bigemina in cattle in the campos de lages region santa catarina state brazil estimated by multiplex pcr |
| url | http://www.sciencedirect.com/science/article/pii/S2405673119300777 |
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