BMP-2 induces versican and hyaluronan that contribute to post-EMT AV cushion cell migration.
Distal outgrowth and maturation of mesenchymalized endocardial cushions are critical morphogenetic events during post-EMT atrioventricular (AV) valvuloseptal morphogenesis. We explored the role of BMP-2 in the regulation of valvulogenic extracellular matrix (ECM) components, versican and hyaluronan...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3795687?pdf=render |
| _version_ | 1811200235526946816 |
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| author | Kei Inai Jessica L Burnside Stanley Hoffman Bryan P Toole Yukiko Sugi |
| author_facet | Kei Inai Jessica L Burnside Stanley Hoffman Bryan P Toole Yukiko Sugi |
| author_sort | Kei Inai |
| collection | DOAJ |
| description | Distal outgrowth and maturation of mesenchymalized endocardial cushions are critical morphogenetic events during post-EMT atrioventricular (AV) valvuloseptal morphogenesis. We explored the role of BMP-2 in the regulation of valvulogenic extracellular matrix (ECM) components, versican and hyaluronan (HA), and cell migration during post-EMT AV cushion distal outgrowth/expansion. We observed intense staining of versican and HA in AV cushion mesenchyme from the early cushion expansion stage, Hamburger and Hamilton (HH) stage-17 to the cushion maturation stage, HH stage-29 in the chick. Based on this expression pattern we examined the role of BMP-2 in regulating versican and HA using 3D AV cushion mesenchymal cell (CMC) aggregate cultures on hydrated collagen gels. BMP-2 induced versican expression and HA deposition as well as mRNA expression of versican and Has2 by CMCs in a dose dependent manner. Noggin, an antagonist of BMP, abolished BMP-2-induced versican and HA as well as mRNA expression of versican and Has2. We further examined whether BMP-2-promoted cell migration was associated with expression of versican and HA. BMP-2- promoted cell migration was significantly impaired by treatments with versican siRNA and HA oligomer. In conclusion, we provide evidence that BMP-2 induces expression of versican and HA by AV CMCs and that these ECM components contribute to BMP-2-induced CMC migration, indicating critical roles for BMP-2 in distal outgrowth/expansion of mesenchymalized AV cushions. |
| first_indexed | 2024-04-12T02:01:10Z |
| format | Article |
| id | doaj.art-4945f755204745b6bafa8eb234245ad2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T02:01:10Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4945f755204745b6bafa8eb234245ad22022-12-22T03:52:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7759310.1371/journal.pone.0077593BMP-2 induces versican and hyaluronan that contribute to post-EMT AV cushion cell migration.Kei InaiJessica L BurnsideStanley HoffmanBryan P TooleYukiko SugiDistal outgrowth and maturation of mesenchymalized endocardial cushions are critical morphogenetic events during post-EMT atrioventricular (AV) valvuloseptal morphogenesis. We explored the role of BMP-2 in the regulation of valvulogenic extracellular matrix (ECM) components, versican and hyaluronan (HA), and cell migration during post-EMT AV cushion distal outgrowth/expansion. We observed intense staining of versican and HA in AV cushion mesenchyme from the early cushion expansion stage, Hamburger and Hamilton (HH) stage-17 to the cushion maturation stage, HH stage-29 in the chick. Based on this expression pattern we examined the role of BMP-2 in regulating versican and HA using 3D AV cushion mesenchymal cell (CMC) aggregate cultures on hydrated collagen gels. BMP-2 induced versican expression and HA deposition as well as mRNA expression of versican and Has2 by CMCs in a dose dependent manner. Noggin, an antagonist of BMP, abolished BMP-2-induced versican and HA as well as mRNA expression of versican and Has2. We further examined whether BMP-2-promoted cell migration was associated with expression of versican and HA. BMP-2- promoted cell migration was significantly impaired by treatments with versican siRNA and HA oligomer. In conclusion, we provide evidence that BMP-2 induces expression of versican and HA by AV CMCs and that these ECM components contribute to BMP-2-induced CMC migration, indicating critical roles for BMP-2 in distal outgrowth/expansion of mesenchymalized AV cushions.http://europepmc.org/articles/PMC3795687?pdf=render |
| spellingShingle | Kei Inai Jessica L Burnside Stanley Hoffman Bryan P Toole Yukiko Sugi BMP-2 induces versican and hyaluronan that contribute to post-EMT AV cushion cell migration. PLoS ONE |
| title | BMP-2 induces versican and hyaluronan that contribute to post-EMT AV cushion cell migration. |
| title_full | BMP-2 induces versican and hyaluronan that contribute to post-EMT AV cushion cell migration. |
| title_fullStr | BMP-2 induces versican and hyaluronan that contribute to post-EMT AV cushion cell migration. |
| title_full_unstemmed | BMP-2 induces versican and hyaluronan that contribute to post-EMT AV cushion cell migration. |
| title_short | BMP-2 induces versican and hyaluronan that contribute to post-EMT AV cushion cell migration. |
| title_sort | bmp 2 induces versican and hyaluronan that contribute to post emt av cushion cell migration |
| url | http://europepmc.org/articles/PMC3795687?pdf=render |
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