Benzocaine and Eugenol as anesthetics for pangasius juveniles, Pangasianodon hypophthalmus

The aim of this study was to evaluate the effect and efficacy of different concentrations of eugenol and benzocaine for pangasius juveniles (Pangasianodon hypophthalmus) and determine the anesthetic and concentration most appropriate for carrying out routine fish farming practices for this species....

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Main Authors: A. F. Leonardo, H. P. Barros, G. G. Sampaio
Format: Article
Language:English
Published: Instituto de Zootecnia 2022-03-01
Series:Boletim de Indústria Animal
Online Access:http://ojs.iz.sp.gov.br/index.php/bia/article/view/1355
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author A. F. Leonardo
H. P. Barros
G. G. Sampaio
author_facet A. F. Leonardo
H. P. Barros
G. G. Sampaio
author_sort A. F. Leonardo
collection DOAJ
description The aim of this study was to evaluate the effect and efficacy of different concentrations of eugenol and benzocaine for pangasius juveniles (Pangasianodon hypophthalmus) and determine the anesthetic and concentration most appropriate for carrying out routine fish farming practices for this species. One hundred juveniles with an average weight of 32.10 ± 4.9 g and an average total length of 15.32 ± 0.57 cm were used. Four concentrations of both anesthetics were evaluated: 0, 25, 50, 75 and 100 mg/L, with four induction time stages and three recovery stages. During the time that the fish remained anesthetized, biometric procedures were performed. Then, they were transferred to a 10-liter aquarium containing clean water, without adding the anesthetic to observe the recovery time. After recovery, the animals were kept in aquariums for 72 hours to check for mortality. Eugenol, at all concentrations evaluated, and benzocaine, at a concentration of 25 mg/L, were not effective in sedating juveniles of pangasius. Benzocaine concentrations of 75 and 100 mg/L were effective for anesthesia and recovery of fish within the time span of five and seven minutes. However, considering animal welfare, the use of a concentration of 100 mg/L is recommended, as it resulted in shorter latency and recovery times.
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spelling doaj.art-4947e5c69f444b9b92a98bbe4a19482f2023-08-08T22:43:29ZengInstituto de ZootecniaBoletim de Indústria Animal1981-41002022-03-0179Benzocaine and Eugenol as anesthetics for pangasius juveniles, Pangasianodon hypophthalmusA. F. LeonardoH. P. BarrosG. G. Sampaio The aim of this study was to evaluate the effect and efficacy of different concentrations of eugenol and benzocaine for pangasius juveniles (Pangasianodon hypophthalmus) and determine the anesthetic and concentration most appropriate for carrying out routine fish farming practices for this species. One hundred juveniles with an average weight of 32.10 ± 4.9 g and an average total length of 15.32 ± 0.57 cm were used. Four concentrations of both anesthetics were evaluated: 0, 25, 50, 75 and 100 mg/L, with four induction time stages and three recovery stages. During the time that the fish remained anesthetized, biometric procedures were performed. Then, they were transferred to a 10-liter aquarium containing clean water, without adding the anesthetic to observe the recovery time. After recovery, the animals were kept in aquariums for 72 hours to check for mortality. Eugenol, at all concentrations evaluated, and benzocaine, at a concentration of 25 mg/L, were not effective in sedating juveniles of pangasius. Benzocaine concentrations of 75 and 100 mg/L were effective for anesthesia and recovery of fish within the time span of five and seven minutes. However, considering animal welfare, the use of a concentration of 100 mg/L is recommended, as it resulted in shorter latency and recovery times. http://ojs.iz.sp.gov.br/index.php/bia/article/view/1355
spellingShingle A. F. Leonardo
H. P. Barros
G. G. Sampaio
Benzocaine and Eugenol as anesthetics for pangasius juveniles, Pangasianodon hypophthalmus
Boletim de Indústria Animal
title Benzocaine and Eugenol as anesthetics for pangasius juveniles, Pangasianodon hypophthalmus
title_full Benzocaine and Eugenol as anesthetics for pangasius juveniles, Pangasianodon hypophthalmus
title_fullStr Benzocaine and Eugenol as anesthetics for pangasius juveniles, Pangasianodon hypophthalmus
title_full_unstemmed Benzocaine and Eugenol as anesthetics for pangasius juveniles, Pangasianodon hypophthalmus
title_short Benzocaine and Eugenol as anesthetics for pangasius juveniles, Pangasianodon hypophthalmus
title_sort benzocaine and eugenol as anesthetics for pangasius juveniles pangasianodon hypophthalmus
url http://ojs.iz.sp.gov.br/index.php/bia/article/view/1355
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AT ggsampaio benzocaineandeugenolasanestheticsforpangasiusjuvenilespangasianodonhypophthalmus