Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficit
IntroductionWater scarcity is a challenge for sesame cultivation under rainfed conditions. In this scenario, a potential strategy to alleviate the water deficit is the application of plant growth-promoting bacteria. The objective of this study was to analyze the interaction of rhizobacteria with ses...
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1324643/full |
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| author | Anderson Reges dos Santos Geisenilma Maria Gonçalves da Rocha Alexandre Paulo Machado Paulo Ivan Fernandes-Junior Nair Helena Castro Arriel Tarcisio Marcos de Souza Gondim Liziane Maria de Lima |
| author_facet | Anderson Reges dos Santos Geisenilma Maria Gonçalves da Rocha Alexandre Paulo Machado Paulo Ivan Fernandes-Junior Nair Helena Castro Arriel Tarcisio Marcos de Souza Gondim Liziane Maria de Lima |
| author_sort | Anderson Reges dos Santos |
| collection | DOAJ |
| description | IntroductionWater scarcity is a challenge for sesame cultivation under rainfed conditions. In this scenario, a potential strategy to alleviate the water deficit is the application of plant growth-promoting bacteria. The objective of this study was to analyze the interaction of rhizobacteria with sesame cultivation under water deficit conditions.MethodsAn experiment was conducted in pots in a greenhouse using the BRS Morena sesame cultivar. The experimental design was completely randomized in a factorial scheme: 2 (irrigation regimes - daily irrigation and water deficit by suspending irrigation until 90% stomatal closure) x 6 (treatments with nitrogen or inoculants), with 5 replications. The types of fertilization were characterized by the addition of nitrogen (ammonium sulfate; 21% N), inoculants based on Bacillus spp. (pant001, ESA 13, and ESA 402), Agrobacterium sp. (ESA 441), and without nitrogen (control). On the fifth day after the suspension of irrigation, plant material was collected for gene expression analysis (DREB1 and HDZ7), activities of antioxidant enzymes (superoxide dismutase and catalase), relative proline content, and photosynthetic pigments. At the end of the crop cycle (about 85 days), production characteristics (root dry matter, aboveground dry matter, number of capsules, and thousand seed weight), as well as leaf nitrogen (N) and phosphorus (P) content, were evaluated.Results and DiscussionThere was a positive effect on both production and biochemical characteristics (proline, superoxide dismutase, catalase, and photosynthetic pigments). Regarding gene expression, most of the inoculated treatments exhibited increased expression of the DREB1 and HDZ7 genes. These biological indicators demonstrate the potential of rhizobacteria for application in sesame cultivation, providing nutritional supply and reducing the effects of water deficit. |
| first_indexed | 2024-03-08T13:18:23Z |
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| id | doaj.art-494b9c7dcd2745a5bde304b4cefd383f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T13:18:23Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-494b9c7dcd2745a5bde304b4cefd383f2024-01-18T04:30:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-01-011410.3389/fpls.2023.13246431324643Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficitAnderson Reges dos Santos0Geisenilma Maria Gonçalves da Rocha1Alexandre Paulo Machado2Paulo Ivan Fernandes-Junior3Nair Helena Castro Arriel4Tarcisio Marcos de Souza Gondim5Liziane Maria de Lima6Master’s Degree in Agricultural Sciences, State University of Paraiba (UEPB), Campina Grande, PB, BrazilPostdoc, Embrapa Algodão, Campina Grande, PB, BrazilDepartment of Basic Health Sciences, Federal University of Mato Grosso, Cuibá, MT, BrazilEmbrapa Semiárido, Petrolina, PE, BrazilEmbrapa Algodão, Campina Grande, PB, BrazilEmbrapa Algodão, Campina Grande, PB, BrazilEmbrapa Algodão, Campina Grande, PB, BrazilIntroductionWater scarcity is a challenge for sesame cultivation under rainfed conditions. In this scenario, a potential strategy to alleviate the water deficit is the application of plant growth-promoting bacteria. The objective of this study was to analyze the interaction of rhizobacteria with sesame cultivation under water deficit conditions.MethodsAn experiment was conducted in pots in a greenhouse using the BRS Morena sesame cultivar. The experimental design was completely randomized in a factorial scheme: 2 (irrigation regimes - daily irrigation and water deficit by suspending irrigation until 90% stomatal closure) x 6 (treatments with nitrogen or inoculants), with 5 replications. The types of fertilization were characterized by the addition of nitrogen (ammonium sulfate; 21% N), inoculants based on Bacillus spp. (pant001, ESA 13, and ESA 402), Agrobacterium sp. (ESA 441), and without nitrogen (control). On the fifth day after the suspension of irrigation, plant material was collected for gene expression analysis (DREB1 and HDZ7), activities of antioxidant enzymes (superoxide dismutase and catalase), relative proline content, and photosynthetic pigments. At the end of the crop cycle (about 85 days), production characteristics (root dry matter, aboveground dry matter, number of capsules, and thousand seed weight), as well as leaf nitrogen (N) and phosphorus (P) content, were evaluated.Results and DiscussionThere was a positive effect on both production and biochemical characteristics (proline, superoxide dismutase, catalase, and photosynthetic pigments). Regarding gene expression, most of the inoculated treatments exhibited increased expression of the DREB1 and HDZ7 genes. These biological indicators demonstrate the potential of rhizobacteria for application in sesame cultivation, providing nutritional supply and reducing the effects of water deficit.https://www.frontiersin.org/articles/10.3389/fpls.2023.1324643/fulldiazotrophic bacteriasymbiosisantioxidant enzymesDREB1 and HDZ7 genesdrought |
| spellingShingle | Anderson Reges dos Santos Geisenilma Maria Gonçalves da Rocha Alexandre Paulo Machado Paulo Ivan Fernandes-Junior Nair Helena Castro Arriel Tarcisio Marcos de Souza Gondim Liziane Maria de Lima Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficit Frontiers in Plant Science diazotrophic bacteria symbiosis antioxidant enzymes DREB1 and HDZ7 genes drought |
| title | Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficit |
| title_full | Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficit |
| title_fullStr | Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficit |
| title_full_unstemmed | Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficit |
| title_short | Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficit |
| title_sort | molecular and biochemical responses of sesame sesame indicum l to rhizobacteria inoculation under water deficit |
| topic | diazotrophic bacteria symbiosis antioxidant enzymes DREB1 and HDZ7 genes drought |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1324643/full |
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