Application of reverse vaccinology to design a multi-epitope subunit vaccine against a new strain of Aeromonas veronii
Abstract Background Aeromonas veronii is one of the most common pathogens of freshwater fishes that cause sepsis and ulcers. There are increasing numbers of cases showing that it is a significant zoonotic and aquatic agent. Epidemiological studies have shown that A. veronii virulence and drug tolera...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-022-00391-8 |
| _version_ | 1797204453884952576 |
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| author | Sk Injamamul Islam Moslema Jahan Mou Saloa Sanjida |
| author_facet | Sk Injamamul Islam Moslema Jahan Mou Saloa Sanjida |
| author_sort | Sk Injamamul Islam |
| collection | DOAJ |
| description | Abstract Background Aeromonas veronii is one of the most common pathogens of freshwater fishes that cause sepsis and ulcers. There are increasing numbers of cases showing that it is a significant zoonotic and aquatic agent. Epidemiological studies have shown that A. veronii virulence and drug tolerance have both increased over the last few years as a result of epidemiological investigations. Cadaverine reverse transporter (CadB) and maltoporin (LamB protein) contribute to the virulence of A. veronii TH0426. TH0426 strain is currently showing severe cases on fish species, and its resistance against therapeutic has been increasing. Despite these devastating complications, there is still no effective cure or vaccine for this strain of A.veronii. Results In this regard, an immunoinformatic method was used to generate an epitope-based vaccine against this pathogen. The immunodominant epitopes were identified using the CadB and LamB protein of A. veronii. The final constructed vaccine sequence was developed to be immunogenic, non-allergenic as well as have better solubility. Molecular dynamic simulation revealed significant binding stability and structural compactness. Finally, using Escherichia coli K12 as a model, codon optimization yielded ideal GC content and a higher CAI value, which was then included in the cloning vector pET2+ (a). Conclusion Altogether, our outcomes imply that the proposed peptide vaccine might be a good option for A. veronii TH0426 prophylaxis. |
| first_indexed | 2024-04-12T06:30:07Z |
| format | Article |
| id | doaj.art-494dee89b80f43939b528535a087bccd |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:35:28Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-494dee89b80f43939b528535a087bccd2024-04-16T16:52:46ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202022-08-0120111810.1186/s43141-022-00391-8Application of reverse vaccinology to design a multi-epitope subunit vaccine against a new strain of Aeromonas veroniiSk Injamamul Islam0Moslema Jahan Mou1Saloa Sanjida2Department of Fisheries and Marine Bioscience, Faculty of Biological Science, Jashore University of Science and TechnologyDepartment of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of RajshahiDepartment of Environmental Science and Technology, Faculty of Applied Science and Technology, Jashore University of Science and TechnologyAbstract Background Aeromonas veronii is one of the most common pathogens of freshwater fishes that cause sepsis and ulcers. There are increasing numbers of cases showing that it is a significant zoonotic and aquatic agent. Epidemiological studies have shown that A. veronii virulence and drug tolerance have both increased over the last few years as a result of epidemiological investigations. Cadaverine reverse transporter (CadB) and maltoporin (LamB protein) contribute to the virulence of A. veronii TH0426. TH0426 strain is currently showing severe cases on fish species, and its resistance against therapeutic has been increasing. Despite these devastating complications, there is still no effective cure or vaccine for this strain of A.veronii. Results In this regard, an immunoinformatic method was used to generate an epitope-based vaccine against this pathogen. The immunodominant epitopes were identified using the CadB and LamB protein of A. veronii. The final constructed vaccine sequence was developed to be immunogenic, non-allergenic as well as have better solubility. Molecular dynamic simulation revealed significant binding stability and structural compactness. Finally, using Escherichia coli K12 as a model, codon optimization yielded ideal GC content and a higher CAI value, which was then included in the cloning vector pET2+ (a). Conclusion Altogether, our outcomes imply that the proposed peptide vaccine might be a good option for A. veronii TH0426 prophylaxis.https://doi.org/10.1186/s43141-022-00391-8A. Veronii TH0426VaccineEpitopesMD simulationE. coli K12 |
| spellingShingle | Sk Injamamul Islam Moslema Jahan Mou Saloa Sanjida Application of reverse vaccinology to design a multi-epitope subunit vaccine against a new strain of Aeromonas veronii Journal of Genetic Engineering and Biotechnology A. Veronii TH0426 Vaccine Epitopes MD simulation E. coli K12 |
| title | Application of reverse vaccinology to design a multi-epitope subunit vaccine against a new strain of Aeromonas veronii |
| title_full | Application of reverse vaccinology to design a multi-epitope subunit vaccine against a new strain of Aeromonas veronii |
| title_fullStr | Application of reverse vaccinology to design a multi-epitope subunit vaccine against a new strain of Aeromonas veronii |
| title_full_unstemmed | Application of reverse vaccinology to design a multi-epitope subunit vaccine against a new strain of Aeromonas veronii |
| title_short | Application of reverse vaccinology to design a multi-epitope subunit vaccine against a new strain of Aeromonas veronii |
| title_sort | application of reverse vaccinology to design a multi epitope subunit vaccine against a new strain of aeromonas veronii |
| topic | A. Veronii TH0426 Vaccine Epitopes MD simulation E. coli K12 |
| url | https://doi.org/10.1186/s43141-022-00391-8 |
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