Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil Compatibilidade vegetativa e caracterização molecular de isolados patogênicos de Fusarium graminearum do Estado do Paraná
Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation...
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| Language: | English |
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Universidade Federal de Santa Maria
2007-12-01
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050 |
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| author | Cleverson Busso Edilson Nobuyoshi Kaneshima Francisco de Assis Franco Carmen Boto Querol Marialba Avezum Alves de Castro-Prado |
| author_facet | Cleverson Busso Edilson Nobuyoshi Kaneshima Francisco de Assis Franco Carmen Boto Querol Marialba Avezum Alves de Castro-Prado |
| author_sort | Cleverson Busso |
| collection | DOAJ |
| description | Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the clonal origin of F. graminearum isolates.<br>Isolados de Fusarium graminearum, obtidos de espigas de trigo com sintomas de Giberela, foram analisados pela técnica do Polimorfismo de DNA Amplificado ao Acaso (RAPD) e pelos Grupos de Compatibilidade Vegetativa (GCV). Mutantes auxotróficos (nit) de cada isolado foram pareados em todas as combinações possíveis, para a formação de heterocários. Três GCVs foram identificados: GCV1, incluindo os isolados F-2, F-3 e F-4; GCV2, incluindo os isolados F-1, F-6 e F-9; e GCV3, formado pelos isolados F-5, F-7 e F-8. Dois grupos foram identificados com base nos marcadores de RAPD: o grupo A, formado pelos isolados F-2 e F-9, e o grupo B, composto pelos demais isolados, os quais apresentaram grande similaridade entre si. Os resultados sugerem a origem clonal dos isolados de F. graminearum analisados. |
| first_indexed | 2024-12-13T16:21:02Z |
| format | Article |
| id | doaj.art-494e5e25c11d4f29980856fdedd0cb6c |
| institution | Directory Open Access Journal |
| issn | 0103-8478 1678-4596 |
| language | English |
| last_indexed | 2024-12-13T16:21:02Z |
| publishDate | 2007-12-01 |
| publisher | Universidade Federal de Santa Maria |
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| series | Ciência Rural |
| spelling | doaj.art-494e5e25c11d4f29980856fdedd0cb6c2022-12-21T23:38:43ZengUniversidade Federal de Santa MariaCiência Rural0103-84781678-45962007-12-013761813181610.1590/S0103-84782007000600050Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil Compatibilidade vegetativa e caracterização molecular de isolados patogênicos de Fusarium graminearum do Estado do ParanáCleverson BussoEdilson Nobuyoshi KaneshimaFrancisco de Assis FrancoCarmen Boto QuerolMarialba Avezum Alves de Castro-PradoFusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the clonal origin of F. graminearum isolates.<br>Isolados de Fusarium graminearum, obtidos de espigas de trigo com sintomas de Giberela, foram analisados pela técnica do Polimorfismo de DNA Amplificado ao Acaso (RAPD) e pelos Grupos de Compatibilidade Vegetativa (GCV). Mutantes auxotróficos (nit) de cada isolado foram pareados em todas as combinações possíveis, para a formação de heterocários. Três GCVs foram identificados: GCV1, incluindo os isolados F-2, F-3 e F-4; GCV2, incluindo os isolados F-1, F-6 e F-9; e GCV3, formado pelos isolados F-5, F-7 e F-8. Dois grupos foram identificados com base nos marcadores de RAPD: o grupo A, formado pelos isolados F-2 e F-9, e o grupo B, composto pelos demais isolados, os quais apresentaram grande similaridade entre si. Os resultados sugerem a origem clonal dos isolados de F. graminearum analisados.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050Fusarium graminearumgrupos de compatibilidade vegetativaRAPDvariabilidade genéticaFusarium graminearumgenetic variabilityRAPDvegetative compatibility groups |
| spellingShingle | Cleverson Busso Edilson Nobuyoshi Kaneshima Francisco de Assis Franco Carmen Boto Querol Marialba Avezum Alves de Castro-Prado Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil Compatibilidade vegetativa e caracterização molecular de isolados patogênicos de Fusarium graminearum do Estado do Paraná Ciência Rural Fusarium graminearum grupos de compatibilidade vegetativa RAPD variabilidade genética Fusarium graminearum genetic variability RAPD vegetative compatibility groups |
| title | Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil Compatibilidade vegetativa e caracterização molecular de isolados patogênicos de Fusarium graminearum do Estado do Paraná |
| title_full | Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil Compatibilidade vegetativa e caracterização molecular de isolados patogênicos de Fusarium graminearum do Estado do Paraná |
| title_fullStr | Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil Compatibilidade vegetativa e caracterização molecular de isolados patogênicos de Fusarium graminearum do Estado do Paraná |
| title_full_unstemmed | Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil Compatibilidade vegetativa e caracterização molecular de isolados patogênicos de Fusarium graminearum do Estado do Paraná |
| title_short | Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil Compatibilidade vegetativa e caracterização molecular de isolados patogênicos de Fusarium graminearum do Estado do Paraná |
| title_sort | vegetative compatibility and molecular characterization of fusarium graminearum isolates from the state of parana brazil compatibilidade vegetativa e caracterizacao molecular de isolados patogenicos de fusarium graminearum do estado do parana |
| topic | Fusarium graminearum grupos de compatibilidade vegetativa RAPD variabilidade genética Fusarium graminearum genetic variability RAPD vegetative compatibility groups |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050 |
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