Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle
Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yi...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/12/5/627 |
| _version_ | 1797498458826866688 |
|---|---|
| author | Yuefang Zhao Lei Yang Guanghua Su Zhuying Wei Xuefei Liu Lishuang Song Chao Hai Di Wu Zhenting Hao Yunxi Wu Li Zhang Chunling Bai Guangpeng Li |
| author_facet | Yuefang Zhao Lei Yang Guanghua Su Zhuying Wei Xuefei Liu Lishuang Song Chao Hai Di Wu Zhenting Hao Yunxi Wu Li Zhang Chunling Bai Guangpeng Li |
| author_sort | Yuefang Zhao |
| collection | DOAJ |
| description | Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yield are significantly inferior. Herein, the myostatin gene (<i>MSTN</i>), a negative regulator of skeletal muscle development, was knocked out by CRISPR/Cas9 technology. Eight <i>MSTN</i> gene-edited bull calves (MT) were born, and six of them are well-developed. Compared with the control cattle (WT), the growth trait indexes of MT cattle were generally increased, and the hindquarters especially were significantly improved. The biochemical indexes and the semen characteristics demonstrated that MT bulls were healthy and fertile. Consistent with our conjecture, the wobble and beating of MT bull spermatozoa were significantly higher than that of WT. Nine sperm motility-related proteins and nineteen mitochondrial-related proteins were identified by up-regulation in MT bull spermatozoa using FLQ proteomic technique and act to govern sperm flagellum assembly, organization, and beating and provide sufficient energy for sperm motility. The current study confirmed that the <i>MSTN</i> gene-edited Chinese Yellow cattle have improved growth traits and normal fertility, which can be used for beef cattle production and breeding. |
| first_indexed | 2024-03-10T03:33:40Z |
| format | Article |
| id | doaj.art-494fb0e80af34c5da9424957c7575f77 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T03:33:40Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-494fb0e80af34c5da9424957c7575f772023-11-23T11:49:00ZengMDPI AGLife2075-17292022-04-0112562710.3390/life12050627Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow CattleYuefang Zhao0Lei Yang1Guanghua Su2Zhuying Wei3Xuefei Liu4Lishuang Song5Chao Hai6Di Wu7Zhenting Hao8Yunxi Wu9Li Zhang10Chunling Bai11Guangpeng Li12State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaChinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yield are significantly inferior. Herein, the myostatin gene (<i>MSTN</i>), a negative regulator of skeletal muscle development, was knocked out by CRISPR/Cas9 technology. Eight <i>MSTN</i> gene-edited bull calves (MT) were born, and six of them are well-developed. Compared with the control cattle (WT), the growth trait indexes of MT cattle were generally increased, and the hindquarters especially were significantly improved. The biochemical indexes and the semen characteristics demonstrated that MT bulls were healthy and fertile. Consistent with our conjecture, the wobble and beating of MT bull spermatozoa were significantly higher than that of WT. Nine sperm motility-related proteins and nineteen mitochondrial-related proteins were identified by up-regulation in MT bull spermatozoa using FLQ proteomic technique and act to govern sperm flagellum assembly, organization, and beating and provide sufficient energy for sperm motility. The current study confirmed that the <i>MSTN</i> gene-edited Chinese Yellow cattle have improved growth traits and normal fertility, which can be used for beef cattle production and breeding.https://www.mdpi.com/2075-1729/12/5/627gene editing cattleMSTNCRISPR/Cas9spermFLQ proteomic |
| spellingShingle | Yuefang Zhao Lei Yang Guanghua Su Zhuying Wei Xuefei Liu Lishuang Song Chao Hai Di Wu Zhenting Hao Yunxi Wu Li Zhang Chunling Bai Guangpeng Li Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle Life gene editing cattle MSTN CRISPR/Cas9 sperm FLQ proteomic |
| title | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
| title_full | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
| title_fullStr | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
| title_full_unstemmed | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
| title_short | Growth Traits and Sperm Proteomics Analyses of Myostatin Gene-Edited Chinese Yellow Cattle |
| title_sort | growth traits and sperm proteomics analyses of myostatin gene edited chinese yellow cattle |
| topic | gene editing cattle MSTN CRISPR/Cas9 sperm FLQ proteomic |
| url | https://www.mdpi.com/2075-1729/12/5/627 |
| work_keys_str_mv | AT yuefangzhao growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT leiyang growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT guanghuasu growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT zhuyingwei growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT xuefeiliu growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT lishuangsong growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT chaohai growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT diwu growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT zhentinghao growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT yunxiwu growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT lizhang growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT chunlingbai growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle AT guangpengli growthtraitsandspermproteomicsanalysesofmyostatingeneeditedchineseyellowcattle |