A Pan-Cancer Landscape of HOX-Related lncRNAs and Their Association With Prognosis and Tumor Microenvironment
The highly conserved homology cassette family (HOX) as well as 18 referenced long non-coding antisense transcripts (HOXATs) play vital roles in the development of some cancers. Nevertheless, their expression patterns as well as their association with cancer prognosis and the tumor microenvironment (...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-11-01
|
| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.767856/full |
| _version_ | 1818982104673812480 |
|---|---|
| author | Wei Shao Qian Ding Yugang Guo Juan Xing Zheng Huo Zhan Wang Qian Xu Yue Guo |
| author_facet | Wei Shao Qian Ding Yugang Guo Juan Xing Zheng Huo Zhan Wang Qian Xu Yue Guo |
| author_sort | Wei Shao |
| collection | DOAJ |
| description | The highly conserved homology cassette family (HOX) as well as 18 referenced long non-coding antisense transcripts (HOXATs) play vital roles in the development of some cancers. Nevertheless, their expression patterns as well as their association with cancer prognosis and the tumor microenvironment (TME) in pan-cancers are still unclear. Here, based on public databases, the expression levels of HOXATs, their prognostic potentials, and correlation with tumor mutation burden (TMB), immune cell infiltration, immune subtype, immune response-related genes, and stemness scores corresponding to 33 tumor types were analyzed systematically using R language. The results of the analysis indicated that different cancer tissues show different HOXAT expression profiles. Further, HOXAT expression showed association with cancer prognosis and immune and stemness regulation. Gene set enrichment analysis also demonstrated that HOXATs participate in cancer- and immune-related pathways, and based on their expression levels, HOTAIRM1 and HOXB-AS1 showed potential involvement in oncogenesis as well as possible involvement in immune regulation across a variety of cancer types. Further investigation also confirmed a significantly higher expression of HOXB-AS1 in GBM than in lower grade glioma tissues. Importantly, in vitro cell function experiments indicated that HOXB-AS1 supports cancer stem cell and plays a fundamental role in glioma metastasis. In conclusion, our results provide valuable resources that can guide the investigation of the mechanisms related to the role of HOXATs in cancers as well as therapeutic analysis in this regard. |
| first_indexed | 2024-12-20T17:41:55Z |
| format | Article |
| id | doaj.art-4950dffe2e02499baef1bb95fc08bc4c |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-20T17:41:55Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-4950dffe2e02499baef1bb95fc08bc4c2022-12-21T19:31:04ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-11-01810.3389/fmolb.2021.767856767856A Pan-Cancer Landscape of HOX-Related lncRNAs and Their Association With Prognosis and Tumor MicroenvironmentWei Shao0Qian Ding1Yugang Guo2Juan Xing3Zheng Huo4Zhan Wang5Qian Xu6Yue Guo7Henan Provincial Engineering Laboratory of Insects Bio-Reactor, Nanyang Normal University, Nanyang, ChinaHenan Provincial Engineering Laboratory of Insects Bio-Reactor, Nanyang Normal University, Nanyang, ChinaHenan Provincial Engineering Laboratory of Insects Bio-Reactor, Nanyang Normal University, Nanyang, ChinaHenan Provincial Nanyang Central Hospital, Nanyang, ChinaHenan Provincial Engineering Laboratory of Insects Bio-Reactor, Nanyang Normal University, Nanyang, ChinaHenan Provincial Engineering Laboratory of Insects Bio-Reactor, Nanyang Normal University, Nanyang, ChinaHenan Provincial Engineering Laboratory of Insects Bio-Reactor, Nanyang Normal University, Nanyang, ChinaHenan Provincial Nanyang Central Hospital, Nanyang, ChinaThe highly conserved homology cassette family (HOX) as well as 18 referenced long non-coding antisense transcripts (HOXATs) play vital roles in the development of some cancers. Nevertheless, their expression patterns as well as their association with cancer prognosis and the tumor microenvironment (TME) in pan-cancers are still unclear. Here, based on public databases, the expression levels of HOXATs, their prognostic potentials, and correlation with tumor mutation burden (TMB), immune cell infiltration, immune subtype, immune response-related genes, and stemness scores corresponding to 33 tumor types were analyzed systematically using R language. The results of the analysis indicated that different cancer tissues show different HOXAT expression profiles. Further, HOXAT expression showed association with cancer prognosis and immune and stemness regulation. Gene set enrichment analysis also demonstrated that HOXATs participate in cancer- and immune-related pathways, and based on their expression levels, HOTAIRM1 and HOXB-AS1 showed potential involvement in oncogenesis as well as possible involvement in immune regulation across a variety of cancer types. Further investigation also confirmed a significantly higher expression of HOXB-AS1 in GBM than in lower grade glioma tissues. Importantly, in vitro cell function experiments indicated that HOXB-AS1 supports cancer stem cell and plays a fundamental role in glioma metastasis. In conclusion, our results provide valuable resources that can guide the investigation of the mechanisms related to the role of HOXATs in cancers as well as therapeutic analysis in this regard.https://www.frontiersin.org/articles/10.3389/fmolb.2021.767856/fullhomology cassette HOXlong non-coding RNAprognostic signaturecancer immunityimmune infiltration |
| spellingShingle | Wei Shao Qian Ding Yugang Guo Juan Xing Zheng Huo Zhan Wang Qian Xu Yue Guo A Pan-Cancer Landscape of HOX-Related lncRNAs and Their Association With Prognosis and Tumor Microenvironment Frontiers in Molecular Biosciences homology cassette HOX long non-coding RNA prognostic signature cancer immunity immune infiltration |
| title | A Pan-Cancer Landscape of HOX-Related lncRNAs and Their Association With Prognosis and Tumor Microenvironment |
| title_full | A Pan-Cancer Landscape of HOX-Related lncRNAs and Their Association With Prognosis and Tumor Microenvironment |
| title_fullStr | A Pan-Cancer Landscape of HOX-Related lncRNAs and Their Association With Prognosis and Tumor Microenvironment |
| title_full_unstemmed | A Pan-Cancer Landscape of HOX-Related lncRNAs and Their Association With Prognosis and Tumor Microenvironment |
| title_short | A Pan-Cancer Landscape of HOX-Related lncRNAs and Their Association With Prognosis and Tumor Microenvironment |
| title_sort | pan cancer landscape of hox related lncrnas and their association with prognosis and tumor microenvironment |
| topic | homology cassette HOX long non-coding RNA prognostic signature cancer immunity immune infiltration |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.767856/full |
| work_keys_str_mv | AT weishao apancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT qianding apancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT yugangguo apancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT juanxing apancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT zhenghuo apancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT zhanwang apancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT qianxu apancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT yueguo apancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT weishao pancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT qianding pancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT yugangguo pancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT juanxing pancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT zhenghuo pancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT zhanwang pancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT qianxu pancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment AT yueguo pancancerlandscapeofhoxrelatedlncrnasandtheirassociationwithprognosisandtumormicroenvironment |