Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana.
The phytohormone gibberellin (GA) is a vital plant signaling molecule that regulates plant growth and defense against abiotic and biotic stresses. To date, the molecular mechanism of the plant responses to viral infection mediated by GA is still undetermined. DELLA is a repressor of GA signaling and...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-04-01
|
| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1010217 |
| _version_ | 1818539870285463552 |
|---|---|
| author | Pengbai Li Liuming Guo Xinyuan Lang Mingjun Li Gentu Wu Rui Wu Lyuxin Wang Meisheng Zhao Ling Qing |
| author_facet | Pengbai Li Liuming Guo Xinyuan Lang Mingjun Li Gentu Wu Rui Wu Lyuxin Wang Meisheng Zhao Ling Qing |
| author_sort | Pengbai Li |
| collection | DOAJ |
| description | The phytohormone gibberellin (GA) is a vital plant signaling molecule that regulates plant growth and defense against abiotic and biotic stresses. To date, the molecular mechanism of the plant responses to viral infection mediated by GA is still undetermined. DELLA is a repressor of GA signaling and is recognized by the F-box protein, a component of the SCFSLY1/GID2 complex. The recognized DELLA is degraded by the ubiquitin-26S proteasome, leading to the activation of GA signaling. Here, we report that ageratum leaf curl Sichuan virus (ALCScV)-infected N. benthamiana plants showed dwarfing symptoms and abnormal flower development. The infection by ALCScV significantly altered the expression of GA pathway-related genes and decreased the content of endogenous GA in N. benthamiana. Furthermore, ALCScV-encoded C4 protein interacts with the DELLA protein NbGAI and interferes with the interaction between NbGAI and NbGID2 to prevent the degradation of NbGAI, leading to inhibition of the GA signaling pathway. Silencing of NbGAI or exogenous GA3 treatment significantly reduces viral accumulation and disease symptoms in N. benthamiana plants. The same results were obtained from experiments with the C4 protein encoded by tobacco curly shoot virus (TbCSV). Therefore, we propose a novel mechanism by which geminivirus C4 proteins control viral infection and disease symptom development by interfering with the GA signaling pathway. |
| first_indexed | 2024-12-11T21:47:49Z |
| format | Article |
| id | doaj.art-49562a9e6b244d37b0ebfdd76fd106f9 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-11T21:47:49Z |
| publishDate | 2022-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-49562a9e6b244d37b0ebfdd76fd106f92022-12-22T00:49:33ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742022-04-01184e101021710.1371/journal.ppat.1010217Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana.Pengbai LiLiuming GuoXinyuan LangMingjun LiGentu WuRui WuLyuxin WangMeisheng ZhaoLing QingThe phytohormone gibberellin (GA) is a vital plant signaling molecule that regulates plant growth and defense against abiotic and biotic stresses. To date, the molecular mechanism of the plant responses to viral infection mediated by GA is still undetermined. DELLA is a repressor of GA signaling and is recognized by the F-box protein, a component of the SCFSLY1/GID2 complex. The recognized DELLA is degraded by the ubiquitin-26S proteasome, leading to the activation of GA signaling. Here, we report that ageratum leaf curl Sichuan virus (ALCScV)-infected N. benthamiana plants showed dwarfing symptoms and abnormal flower development. The infection by ALCScV significantly altered the expression of GA pathway-related genes and decreased the content of endogenous GA in N. benthamiana. Furthermore, ALCScV-encoded C4 protein interacts with the DELLA protein NbGAI and interferes with the interaction between NbGAI and NbGID2 to prevent the degradation of NbGAI, leading to inhibition of the GA signaling pathway. Silencing of NbGAI or exogenous GA3 treatment significantly reduces viral accumulation and disease symptoms in N. benthamiana plants. The same results were obtained from experiments with the C4 protein encoded by tobacco curly shoot virus (TbCSV). Therefore, we propose a novel mechanism by which geminivirus C4 proteins control viral infection and disease symptom development by interfering with the GA signaling pathway.https://doi.org/10.1371/journal.ppat.1010217 |
| spellingShingle | Pengbai Li Liuming Guo Xinyuan Lang Mingjun Li Gentu Wu Rui Wu Lyuxin Wang Meisheng Zhao Ling Qing Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana. PLoS Pathogens |
| title | Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana. |
| title_full | Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana. |
| title_fullStr | Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana. |
| title_full_unstemmed | Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana. |
| title_short | Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana. |
| title_sort | geminivirus c4 proteins inhibit ga signaling via prevention of nbgai degradation to promote viral infection and symptom development in n benthamiana |
| url | https://doi.org/10.1371/journal.ppat.1010217 |
| work_keys_str_mv | AT pengbaili geminivirusc4proteinsinhibitgasignalingviapreventionofnbgaidegradationtopromoteviralinfectionandsymptomdevelopmentinnbenthamiana AT liumingguo geminivirusc4proteinsinhibitgasignalingviapreventionofnbgaidegradationtopromoteviralinfectionandsymptomdevelopmentinnbenthamiana AT xinyuanlang geminivirusc4proteinsinhibitgasignalingviapreventionofnbgaidegradationtopromoteviralinfectionandsymptomdevelopmentinnbenthamiana AT mingjunli geminivirusc4proteinsinhibitgasignalingviapreventionofnbgaidegradationtopromoteviralinfectionandsymptomdevelopmentinnbenthamiana AT gentuwu geminivirusc4proteinsinhibitgasignalingviapreventionofnbgaidegradationtopromoteviralinfectionandsymptomdevelopmentinnbenthamiana AT ruiwu geminivirusc4proteinsinhibitgasignalingviapreventionofnbgaidegradationtopromoteviralinfectionandsymptomdevelopmentinnbenthamiana AT lyuxinwang geminivirusc4proteinsinhibitgasignalingviapreventionofnbgaidegradationtopromoteviralinfectionandsymptomdevelopmentinnbenthamiana AT meishengzhao geminivirusc4proteinsinhibitgasignalingviapreventionofnbgaidegradationtopromoteviralinfectionandsymptomdevelopmentinnbenthamiana AT lingqing geminivirusc4proteinsinhibitgasignalingviapreventionofnbgaidegradationtopromoteviralinfectionandsymptomdevelopmentinnbenthamiana |