Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP
Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2012-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400011 |
| _version_ | 1818429577578414080 |
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| author | Ashwin A. Raut Anil Kumar Sheo N. Kala Vinod Chhokar Neeraj Rana Vikas Beniwal Sundeep Jaglan Sachin K. Samuchiwal Jitender K. Singh Anamika Mishra |
| author_facet | Ashwin A. Raut Anil Kumar Sheo N. Kala Vinod Chhokar Neeraj Rana Vikas Beniwal Sundeep Jaglan Sachin K. Samuchiwal Jitender K. Singh Anamika Mishra |
| author_sort | Ashwin A. Raut |
| collection | DOAJ |
| description | Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may influence DGAT1 expression and function. The results described here could be useful in examining the association between the DGAT1 gene and milk traits in buffalo. |
| first_indexed | 2024-12-14T15:19:44Z |
| format | Article |
| id | doaj.art-4957223c512544ed8ca01d973e06d03b |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-14T15:19:44Z |
| publishDate | 2012-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-4957223c512544ed8ca01d973e06d03b2022-12-21T22:56:13ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852012-01-01353610613Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCPAshwin A. RautAnil KumarSheo N. KalaVinod ChhokarNeeraj RanaVikas BeniwalSundeep JaglanSachin K. SamuchiwalJitender K. SinghAnamika MishraDiacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may influence DGAT1 expression and function. The results described here could be useful in examining the association between the DGAT1 gene and milk traits in buffalo.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400011buffaloDGAT1PCR-SSCPpolymorphismSNP |
| spellingShingle | Ashwin A. Raut Anil Kumar Sheo N. Kala Vinod Chhokar Neeraj Rana Vikas Beniwal Sundeep Jaglan Sachin K. Samuchiwal Jitender K. Singh Anamika Mishra Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP Genetics and Molecular Biology buffalo DGAT1 PCR-SSCP polymorphism SNP |
| title | Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP |
| title_full | Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP |
| title_fullStr | Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP |
| title_full_unstemmed | Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP |
| title_short | Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP |
| title_sort | identification of novel single nucleotide polymorphisms in the dgat1 gene of buffaloes by pcr sscp |
| topic | buffalo DGAT1 PCR-SSCP polymorphism SNP |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400011 |
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