Ookinete-Specific Genes and 18S SSU rRNA Evidenced in Plasmodium vivax Selection and Adaptation by Sympatric Vectors
In the southern Pacific coast of Chiapas, Mexico (SM), the two most abundant vector species, Nyssorhynchus albimanus and Anopheles pseudopunctipennis, were susceptible to different Plasmodium vivax Pvs25/28 haplotypes. To broaden our understanding of the existing P. vivax in the area, genes encoding...
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.01362/full |
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| author | Lilia González-Cerón Mario H. Rodríguez Marbella T. Ovilla-Muñoz Frida Santillán-Valenzuela Juan E. Hernández-Ávila María Carmen Rodríguez Jesús Martínez- Barnetche Cuauhtémoc Villarreal-Treviño |
| author_facet | Lilia González-Cerón Mario H. Rodríguez Marbella T. Ovilla-Muñoz Frida Santillán-Valenzuela Juan E. Hernández-Ávila María Carmen Rodríguez Jesús Martínez- Barnetche Cuauhtémoc Villarreal-Treviño |
| author_sort | Lilia González-Cerón |
| collection | DOAJ |
| description | In the southern Pacific coast of Chiapas, Mexico (SM), the two most abundant vector species, Nyssorhynchus albimanus and Anopheles pseudopunctipennis, were susceptible to different Plasmodium vivax Pvs25/28 haplotypes. To broaden our understanding of the existing P. vivax in the area, genes encoding proteins relevant for ookinete development and the 18S rRNA were studied. P. vivax infectivity (percentage of infected mosquitoes and oocyst numbers) was evaluated by simultaneously feeding infected blood samples from patients to Ny. albimanus and An. pseudopunctipennis female mosquitoes. Three infectivity patterns were identified: one group of parasites were more infective to An. pseudopunctipennis than to Ny. albimanus, another group was more infective to Ny. albimanus, while a third group infected both vectors similarly. In 29 parasite isolates, the molecular variations of ookinete-specific genes and the 18S rRNA-type S were analyzed. Using concatenated sequences, phylogenetic trees, and Structure analysis, parasite clustering within SM isolates and between these and those from other geographical origins were investigated. A ML phylogenetic tree resolved two parasite lineages: PvSM-A and PvSM-B. They were associated to a different 18S rRNA variant. PvSM-A parasites had 18S rRNA variant rV2 and correspond to parasites causing high oocyst infection in Ny. albimanus. A new ML tree and Structure analysis, both comprising global sequences, showed PvSM-A clustered with Latin American parasites. Meanwhile, all isolates of PvSM-B had 18S rRNA variant rV1 and remained as unique genetic cluster comprising two subgroups: PvSM-Ba, producing high infection in An. pseudopunctipennis, and PvSM-Bb, causing similar oocyst infection in both vector species. PvSM-A parasites were genetically similar to parasites from South America. Meanwhile, PvSM-B were exclusive to southern Mexico and share ancestry with Asian parasites. The results suggest that these lineages evolved separately, likely by geographic and vector restriction. |
| first_indexed | 2024-12-21T01:26:10Z |
| format | Article |
| id | doaj.art-4958d4a5c4b9468baf0388e39ed1fb76 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-21T01:26:10Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-4958d4a5c4b9468baf0388e39ed1fb762022-12-21T19:20:30ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-02-011010.3389/fgene.2019.01362496240Ookinete-Specific Genes and 18S SSU rRNA Evidenced in Plasmodium vivax Selection and Adaptation by Sympatric VectorsLilia González-Cerón0Mario H. Rodríguez1Marbella T. Ovilla-Muñoz2Frida Santillán-Valenzuela3Juan E. Hernández-Ávila4María Carmen Rodríguez5Jesús Martínez- Barnetche6Cuauhtémoc Villarreal-Treviño7Regional Center of Research in Public Health, National Institute of Public Health, Ministry of Health, Tapachula, MexicoVector Borne Diseases, Center for Research on Infectious Diseases, National Institute of Public Health, Ministry of Health, Cuernavaca, MexicoChronic Infections and Cancer, Center for Research on Infectious Diseases, National Institute of Public Health, Ministry of Health, Cuernavaca, MexicoRegional Center of Research in Public Health, National Institute of Public Health, Ministry of Health, Tapachula, MexicoCenter of Information for Public Health Decisions, National Institute of Public Health, Ministry of Health, Mexico City, MexicoVector Borne Diseases, Center for Research on Infectious Diseases, National Institute of Public Health, Ministry of Health, Cuernavaca, MexicoChronic Infections and Cancer, Center for Research on Infectious Diseases, National Institute of Public Health, Ministry of Health, Cuernavaca, MexicoRegional Center of Research in Public Health, National Institute of Public Health, Ministry of Health, Tapachula, MexicoIn the southern Pacific coast of Chiapas, Mexico (SM), the two most abundant vector species, Nyssorhynchus albimanus and Anopheles pseudopunctipennis, were susceptible to different Plasmodium vivax Pvs25/28 haplotypes. To broaden our understanding of the existing P. vivax in the area, genes encoding proteins relevant for ookinete development and the 18S rRNA were studied. P. vivax infectivity (percentage of infected mosquitoes and oocyst numbers) was evaluated by simultaneously feeding infected blood samples from patients to Ny. albimanus and An. pseudopunctipennis female mosquitoes. Three infectivity patterns were identified: one group of parasites were more infective to An. pseudopunctipennis than to Ny. albimanus, another group was more infective to Ny. albimanus, while a third group infected both vectors similarly. In 29 parasite isolates, the molecular variations of ookinete-specific genes and the 18S rRNA-type S were analyzed. Using concatenated sequences, phylogenetic trees, and Structure analysis, parasite clustering within SM isolates and between these and those from other geographical origins were investigated. A ML phylogenetic tree resolved two parasite lineages: PvSM-A and PvSM-B. They were associated to a different 18S rRNA variant. PvSM-A parasites had 18S rRNA variant rV2 and correspond to parasites causing high oocyst infection in Ny. albimanus. A new ML tree and Structure analysis, both comprising global sequences, showed PvSM-A clustered with Latin American parasites. Meanwhile, all isolates of PvSM-B had 18S rRNA variant rV1 and remained as unique genetic cluster comprising two subgroups: PvSM-Ba, producing high infection in An. pseudopunctipennis, and PvSM-Bb, causing similar oocyst infection in both vector species. PvSM-A parasites were genetically similar to parasites from South America. Meanwhile, PvSM-B were exclusive to southern Mexico and share ancestry with Asian parasites. The results suggest that these lineages evolved separately, likely by geographic and vector restriction.https://www.frontiersin.org/article/10.3389/fgene.2019.01362/fullPlasmodium vivaxNyssorhynchus albimanusAnopheles pseudopunctipennisookinete proteinsphylogenetic tree18S SSU rRNA |
| spellingShingle | Lilia González-Cerón Mario H. Rodríguez Marbella T. Ovilla-Muñoz Frida Santillán-Valenzuela Juan E. Hernández-Ávila María Carmen Rodríguez Jesús Martínez- Barnetche Cuauhtémoc Villarreal-Treviño Ookinete-Specific Genes and 18S SSU rRNA Evidenced in Plasmodium vivax Selection and Adaptation by Sympatric Vectors Frontiers in Genetics Plasmodium vivax Nyssorhynchus albimanus Anopheles pseudopunctipennis ookinete proteins phylogenetic tree 18S SSU rRNA |
| title | Ookinete-Specific Genes and 18S SSU rRNA Evidenced in Plasmodium vivax Selection and Adaptation by Sympatric Vectors |
| title_full | Ookinete-Specific Genes and 18S SSU rRNA Evidenced in Plasmodium vivax Selection and Adaptation by Sympatric Vectors |
| title_fullStr | Ookinete-Specific Genes and 18S SSU rRNA Evidenced in Plasmodium vivax Selection and Adaptation by Sympatric Vectors |
| title_full_unstemmed | Ookinete-Specific Genes and 18S SSU rRNA Evidenced in Plasmodium vivax Selection and Adaptation by Sympatric Vectors |
| title_short | Ookinete-Specific Genes and 18S SSU rRNA Evidenced in Plasmodium vivax Selection and Adaptation by Sympatric Vectors |
| title_sort | ookinete specific genes and 18s ssu rrna evidenced in plasmodium vivax selection and adaptation by sympatric vectors |
| topic | Plasmodium vivax Nyssorhynchus albimanus Anopheles pseudopunctipennis ookinete proteins phylogenetic tree 18S SSU rRNA |
| url | https://www.frontiersin.org/article/10.3389/fgene.2019.01362/full |
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