Less demand on stem cell marker-positive cancer cells may characterize metastasis of colon cancer
<h4>Background</h4> CD44 and CD133 are stem cell markers in colorectal cancer (CRC). CD44 has distinctive isoforms with different oncological properties like total CD44 (CD44T) and variant CD44 (CD44V). Clinical significance of such markers remains elusive. <h4>Methods</h4> S...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128954/?tool=EBI |
| _version_ | 1827958219253743616 |
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| author | Takeshi Kaida Yoshiki Fujiyama Takafumi Soeno Mitsuo Yokota Shuji Nakamoto Takuya Goto Akiko Watanabe Kota Okuno Yusuke Nie Shiori Fujino Kazuko Yokota Hiroki Harada Yoko Tanaka Toshimichi Tanaka Keigo Yokoi Ken Kojo Hirohisa Miura Takahiro Yamanashi Takeo Sato Jiichiro Sasaki Takafumi Sangai Naoki Hiki Yusuke Kumamoto Takeshi Naitoh Keishi Yamashita |
| author_facet | Takeshi Kaida Yoshiki Fujiyama Takafumi Soeno Mitsuo Yokota Shuji Nakamoto Takuya Goto Akiko Watanabe Kota Okuno Yusuke Nie Shiori Fujino Kazuko Yokota Hiroki Harada Yoko Tanaka Toshimichi Tanaka Keigo Yokoi Ken Kojo Hirohisa Miura Takahiro Yamanashi Takeo Sato Jiichiro Sasaki Takafumi Sangai Naoki Hiki Yusuke Kumamoto Takeshi Naitoh Keishi Yamashita |
| author_sort | Takeshi Kaida |
| collection | DOAJ |
| description | <h4>Background</h4> CD44 and CD133 are stem cell markers in colorectal cancer (CRC). CD44 has distinctive isoforms with different oncological properties like total CD44 (CD44T) and variant CD44 (CD44V). Clinical significance of such markers remains elusive. <h4>Methods</h4> Sixty colon cancer were examined for CD44T/CD44V and CD133 at mRNA level in a quantitative PCR, and clarified for their association with clinicopathological factors. <h4>Results</h4> (1) Both CD44T and CD44V showed higher expression in primary colon tumors than in non-cancerous mucosas (p<0.0001), while CD133 was expressed even in non-cancerous mucosa and rather decreased in the tumors (p = 0.048). (2) CD44V expression was significantly associated with CD44T expression (R = 0.62, p<0.0001), while they were not correlated to CD133 at all in the primary tumors. (3) CD44V/CD44T expressions were significantly higher in right colon cancer than in left colon cancer (p = 0.035/p = 0.012, respectively), while CD133 expression were not (p = 0.20). (4) In primary tumors, unexpectedly, CD44V/CD44T/CD133 mRNA expressions were not correlated with aggressive phenotypes, but CD44V/CD44T rather significantly with less aggressive lymph node metastasis/distant metastasis (p = 0.040/p = 0.039, respectively). Moreover, both CD44V and CD133 expressions were significantly decreased in liver metastasis as compared to primary tumors (p = 0.0005 and p = 0.0006, respectively). <h4>Conclusion</h4> Our transcript expression analysis of cancer stem cell markers did not conclude that their expression could represent aggressive phenotypes of primary and metastatic tumors, and rather represented less demand on stem cell marker-positive cancer cells. |
| first_indexed | 2024-04-09T15:32:09Z |
| format | Article |
| id | doaj.art-495a806b6df348429d17ec0ff949c535 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T15:32:09Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-495a806b6df348429d17ec0ff949c5352023-04-28T05:31:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01184Less demand on stem cell marker-positive cancer cells may characterize metastasis of colon cancerTakeshi KaidaYoshiki FujiyamaTakafumi SoenoMitsuo YokotaShuji NakamotoTakuya GotoAkiko WatanabeKota OkunoYusuke NieShiori FujinoKazuko YokotaHiroki HaradaYoko TanakaToshimichi TanakaKeigo YokoiKen KojoHirohisa MiuraTakahiro YamanashiTakeo SatoJiichiro SasakiTakafumi SangaiNaoki HikiYusuke KumamotoTakeshi NaitohKeishi Yamashita<h4>Background</h4> CD44 and CD133 are stem cell markers in colorectal cancer (CRC). CD44 has distinctive isoforms with different oncological properties like total CD44 (CD44T) and variant CD44 (CD44V). Clinical significance of such markers remains elusive. <h4>Methods</h4> Sixty colon cancer were examined for CD44T/CD44V and CD133 at mRNA level in a quantitative PCR, and clarified for their association with clinicopathological factors. <h4>Results</h4> (1) Both CD44T and CD44V showed higher expression in primary colon tumors than in non-cancerous mucosas (p<0.0001), while CD133 was expressed even in non-cancerous mucosa and rather decreased in the tumors (p = 0.048). (2) CD44V expression was significantly associated with CD44T expression (R = 0.62, p<0.0001), while they were not correlated to CD133 at all in the primary tumors. (3) CD44V/CD44T expressions were significantly higher in right colon cancer than in left colon cancer (p = 0.035/p = 0.012, respectively), while CD133 expression were not (p = 0.20). (4) In primary tumors, unexpectedly, CD44V/CD44T/CD133 mRNA expressions were not correlated with aggressive phenotypes, but CD44V/CD44T rather significantly with less aggressive lymph node metastasis/distant metastasis (p = 0.040/p = 0.039, respectively). Moreover, both CD44V and CD133 expressions were significantly decreased in liver metastasis as compared to primary tumors (p = 0.0005 and p = 0.0006, respectively). <h4>Conclusion</h4> Our transcript expression analysis of cancer stem cell markers did not conclude that their expression could represent aggressive phenotypes of primary and metastatic tumors, and rather represented less demand on stem cell marker-positive cancer cells.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128954/?tool=EBI |
| spellingShingle | Takeshi Kaida Yoshiki Fujiyama Takafumi Soeno Mitsuo Yokota Shuji Nakamoto Takuya Goto Akiko Watanabe Kota Okuno Yusuke Nie Shiori Fujino Kazuko Yokota Hiroki Harada Yoko Tanaka Toshimichi Tanaka Keigo Yokoi Ken Kojo Hirohisa Miura Takahiro Yamanashi Takeo Sato Jiichiro Sasaki Takafumi Sangai Naoki Hiki Yusuke Kumamoto Takeshi Naitoh Keishi Yamashita Less demand on stem cell marker-positive cancer cells may characterize metastasis of colon cancer PLoS ONE |
| title | Less demand on stem cell marker-positive cancer cells may characterize metastasis of colon cancer |
| title_full | Less demand on stem cell marker-positive cancer cells may characterize metastasis of colon cancer |
| title_fullStr | Less demand on stem cell marker-positive cancer cells may characterize metastasis of colon cancer |
| title_full_unstemmed | Less demand on stem cell marker-positive cancer cells may characterize metastasis of colon cancer |
| title_short | Less demand on stem cell marker-positive cancer cells may characterize metastasis of colon cancer |
| title_sort | less demand on stem cell marker positive cancer cells may characterize metastasis of colon cancer |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128954/?tool=EBI |
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