Alterations in microRNA Expression during Hematopoietic Stem Cell Mobilization
microRNAs play an important role in the regulation of gene expression, cell fate, hematopoiesis, and may influence the efficacy of CD34+ cell mobilization. The present study examines the role of hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-34a-5p, hsa-miR-126-3p, hsa-miR-146a-5p, hsa-miR-155-5p, and hsa-m...
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MDPI AG
2021-07-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/7/668 |
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| author | Mateusz Nowicki Janusz Szemraj Agnieszka Wierzbowska Agnieszka Pluta Olga Grzybowska-Izydorczyk Aleksandra Nowicka Piotr Stelmach Magdalena Czemerska Anna Szmigielska-Kapłon |
| author_facet | Mateusz Nowicki Janusz Szemraj Agnieszka Wierzbowska Agnieszka Pluta Olga Grzybowska-Izydorczyk Aleksandra Nowicka Piotr Stelmach Magdalena Czemerska Anna Szmigielska-Kapłon |
| author_sort | Mateusz Nowicki |
| collection | DOAJ |
| description | microRNAs play an important role in the regulation of gene expression, cell fate, hematopoiesis, and may influence the efficacy of CD34+ cell mobilization. The present study examines the role of hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-34a-5p, hsa-miR-126-3p, hsa-miR-146a-5p, hsa-miR-155-5p, and hsa-miR-223-3p in the course of hematopoietic stem cell mobilization. The numbers of CD34+ cells collected in patients with hematological malignancies (39 multiple myelomas, 11 lymphomas) were determined during mobilization for an autologous hematopoietic stem cell transplantation. The miRNA level was evaluated by RT-PCR. Compared to baseline, a significant decline in hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-126-3p, hsa-miR-146a-5p, and hsa-miR-155-5p was observed on the day of the first apheresis (day A). An increase was observed only in the expression of hsa-miR-34a-5p. On day A, a negative correlation was found between hsa-miR-15a-5p and hsa-miR-146a-5p levels and the number of CD34+ cells in peripheral blood. A negative correlation was observed between hsa-miR-146a-5p and the number of collected CD34+ cells after the first apheresis. Good mobilizers, defined according to GITMO criteria, demonstrated a lower hsa-miR-146a-5p level on day A than poor mobilizers. Patients from the hsa-miR-146a-5p “low expressors” collected more CD34+ cells than “high expressors”. Our results suggest that the investigated miRNAs, especially hsa-miR-146a-5p, may influence the efficacy of HSC mobilization. |
| first_indexed | 2024-03-10T09:44:53Z |
| format | Article |
| id | doaj.art-495abd41adb341fba5600709d4e115ce |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T09:44:53Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-495abd41adb341fba5600709d4e115ce2023-11-22T03:16:14ZengMDPI AGBiology2079-77372021-07-0110766810.3390/biology10070668Alterations in microRNA Expression during Hematopoietic Stem Cell MobilizationMateusz Nowicki0Janusz Szemraj1Agnieszka Wierzbowska2Agnieszka Pluta3Olga Grzybowska-Izydorczyk4Aleksandra Nowicka5Piotr Stelmach6Magdalena Czemerska7Anna Szmigielska-Kapłon8Department of Hematology, Copernicus Memorial Hospital in Lodz Comprehensive Cancer Center and Traumatology, 93-513 Łódź, PolandDepartment of Medical Biochemistry, Medical University of Lodz, 92-215 Łódź, PolandDepartment of Hematology, Copernicus Memorial Hospital in Lodz Comprehensive Cancer Center and Traumatology, 93-513 Łódź, PolandDepartment of Hematology, Copernicus Memorial Hospital in Lodz Comprehensive Cancer Center and Traumatology, 93-513 Łódź, PolandDepartment of Hematology, Copernicus Memorial Hospital in Lodz Comprehensive Cancer Center and Traumatology, 93-513 Łódź, PolandDepartment of Medical Biochemistry, Medical University of Lodz, 92-215 Łódź, PolandDepartment of Hematology, Copernicus Memorial Hospital in Lodz Comprehensive Cancer Center and Traumatology, 93-513 Łódź, PolandDepartment of Hematology, Copernicus Memorial Hospital in Lodz Comprehensive Cancer Center and Traumatology, 93-513 Łódź, PolandDepartment of Hematology, Copernicus Memorial Hospital in Lodz Comprehensive Cancer Center and Traumatology, 93-513 Łódź, PolandmicroRNAs play an important role in the regulation of gene expression, cell fate, hematopoiesis, and may influence the efficacy of CD34+ cell mobilization. The present study examines the role of hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-34a-5p, hsa-miR-126-3p, hsa-miR-146a-5p, hsa-miR-155-5p, and hsa-miR-223-3p in the course of hematopoietic stem cell mobilization. The numbers of CD34+ cells collected in patients with hematological malignancies (39 multiple myelomas, 11 lymphomas) were determined during mobilization for an autologous hematopoietic stem cell transplantation. The miRNA level was evaluated by RT-PCR. Compared to baseline, a significant decline in hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-126-3p, hsa-miR-146a-5p, and hsa-miR-155-5p was observed on the day of the first apheresis (day A). An increase was observed only in the expression of hsa-miR-34a-5p. On day A, a negative correlation was found between hsa-miR-15a-5p and hsa-miR-146a-5p levels and the number of CD34+ cells in peripheral blood. A negative correlation was observed between hsa-miR-146a-5p and the number of collected CD34+ cells after the first apheresis. Good mobilizers, defined according to GITMO criteria, demonstrated a lower hsa-miR-146a-5p level on day A than poor mobilizers. Patients from the hsa-miR-146a-5p “low expressors” collected more CD34+ cells than “high expressors”. Our results suggest that the investigated miRNAs, especially hsa-miR-146a-5p, may influence the efficacy of HSC mobilization.https://www.mdpi.com/2079-7737/10/7/668miRNAhsa-miR-146a-5pmobilizationCD34+multiple myelomahematopoietic stem cells |
| spellingShingle | Mateusz Nowicki Janusz Szemraj Agnieszka Wierzbowska Agnieszka Pluta Olga Grzybowska-Izydorczyk Aleksandra Nowicka Piotr Stelmach Magdalena Czemerska Anna Szmigielska-Kapłon Alterations in microRNA Expression during Hematopoietic Stem Cell Mobilization Biology miRNA hsa-miR-146a-5p mobilization CD34+ multiple myeloma hematopoietic stem cells |
| title | Alterations in microRNA Expression during Hematopoietic Stem Cell Mobilization |
| title_full | Alterations in microRNA Expression during Hematopoietic Stem Cell Mobilization |
| title_fullStr | Alterations in microRNA Expression during Hematopoietic Stem Cell Mobilization |
| title_full_unstemmed | Alterations in microRNA Expression during Hematopoietic Stem Cell Mobilization |
| title_short | Alterations in microRNA Expression during Hematopoietic Stem Cell Mobilization |
| title_sort | alterations in microrna expression during hematopoietic stem cell mobilization |
| topic | miRNA hsa-miR-146a-5p mobilization CD34+ multiple myeloma hematopoietic stem cells |
| url | https://www.mdpi.com/2079-7737/10/7/668 |
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