Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay
Oral health is maintained by a healthy microbiome, which can be monitored by state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (<i>P. gingivalis</i>, <i>T. forsythia</i>, <i>T. denticola</i>...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/9/2945 |
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| author | Pune N. Paqué Christopher Herz Joël S. Jenzer Daniel B. Wiedemeier Thomas Attin Nagihan Bostanci Georgios N. Belibasakis Kai Bao Philipp Körner Tanja Fritz Julia Prinz Patrick R. Schmidlin Thomas Thurnheer Florian J. Wegehaupt Konstantinos Mitsakakis Johannes R. Peham |
| author_facet | Pune N. Paqué Christopher Herz Joël S. Jenzer Daniel B. Wiedemeier Thomas Attin Nagihan Bostanci Georgios N. Belibasakis Kai Bao Philipp Körner Tanja Fritz Julia Prinz Patrick R. Schmidlin Thomas Thurnheer Florian J. Wegehaupt Konstantinos Mitsakakis Johannes R. Peham |
| author_sort | Pune N. Paqué |
| collection | DOAJ |
| description | Oral health is maintained by a healthy microbiome, which can be monitored by state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (<i>P. gingivalis</i>, <i>T. forsythia</i>, <i>T. denticola</i>, <i>F. nucleatum, C. rectus</i>, <i>P. intermedia</i>, <i>A. actinomycetemcomitans</i>, <i>S. mutans</i>, <i>S. sobrinus</i>, oral associated <i>Lactobacilli</i>) in saliva and their clinical status association in 214 individuals. Upon clinical examination, study subjects were grouped into healthy, caries and periodontitis and their saliva was collected. A highly specific point-of-care compatible dual color qPCR assay was developed and used to study the above-mentioned bacteria of interest in the collected saliva. Assay performance was compared to a commercially available microbial reference test. Eight out of ten taxa that were investigated during this study were strong discriminators between the periodontitis and healthy groups: <i>C. rectus</i>, <i>T. forsythia</i>, <i>P. gingivalis</i>, <i>S. mutans, F. nucleatum</i>, <i>T. denticola</i>, <i>P. intermedia</i> and oral <i>Lactobacilli</i> (<i>p</i> < 0.05). Significant differentiation between the periodontitis and caries group microbiome was only shown for <i>S. mutans</i> (<i>p</i> < 0.05). A clear distinction between oral health and disease was enabled by the analysis of quantitative qPCR data of target taxa levels in saliva. |
| first_indexed | 2024-03-10T16:23:26Z |
| format | Article |
| id | doaj.art-495afa820a894d799e1e6655ed934394 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T16:23:26Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-495afa820a894d799e1e6655ed9343942023-11-20T13:28:55ZengMDPI AGJournal of Clinical Medicine2077-03832020-09-0199294510.3390/jcm9092945Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR AssayPune N. Paqué0Christopher Herz1Joël S. Jenzer2Daniel B. Wiedemeier3Thomas Attin4Nagihan Bostanci5Georgios N. Belibasakis6Kai Bao7Philipp Körner8Tanja Fritz9Julia Prinz10Patrick R. Schmidlin11Thomas Thurnheer12Florian J. Wegehaupt13Konstantinos Mitsakakis14Johannes R. Peham15Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, SwitzerlandAustrian Institute of Technology, Molecular Diagnostics, Giefinggasse 4, 1210 Wien, AustriaClinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, SwitzerlandStatistical Services, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, SwitzerlandClinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, SwitzerlandDivision of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, 141 04 Huddinge, SwedenDivision of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, 141 04 Huddinge, SwedenDivision of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, 141 04 Huddinge, SwedenClinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, SwitzerlandAustrian Institute of Technology, Molecular Diagnostics, Giefinggasse 4, 1210 Wien, AustriaAustrian Institute of Technology, Molecular Diagnostics, Giefinggasse 4, 1210 Wien, AustriaClinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, SwitzerlandClinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, SwitzerlandClinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, SwitzerlandHahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, GermanyAustrian Institute of Technology, Molecular Diagnostics, Giefinggasse 4, 1210 Wien, AustriaOral health is maintained by a healthy microbiome, which can be monitored by state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (<i>P. gingivalis</i>, <i>T. forsythia</i>, <i>T. denticola</i>, <i>F. nucleatum, C. rectus</i>, <i>P. intermedia</i>, <i>A. actinomycetemcomitans</i>, <i>S. mutans</i>, <i>S. sobrinus</i>, oral associated <i>Lactobacilli</i>) in saliva and their clinical status association in 214 individuals. Upon clinical examination, study subjects were grouped into healthy, caries and periodontitis and their saliva was collected. A highly specific point-of-care compatible dual color qPCR assay was developed and used to study the above-mentioned bacteria of interest in the collected saliva. Assay performance was compared to a commercially available microbial reference test. Eight out of ten taxa that were investigated during this study were strong discriminators between the periodontitis and healthy groups: <i>C. rectus</i>, <i>T. forsythia</i>, <i>P. gingivalis</i>, <i>S. mutans, F. nucleatum</i>, <i>T. denticola</i>, <i>P. intermedia</i> and oral <i>Lactobacilli</i> (<i>p</i> < 0.05). Significant differentiation between the periodontitis and caries group microbiome was only shown for <i>S. mutans</i> (<i>p</i> < 0.05). A clear distinction between oral health and disease was enabled by the analysis of quantitative qPCR data of target taxa levels in saliva.https://www.mdpi.com/2077-0383/9/9/2945point of carecariesperiodontitisoral healthsaliva diagnosticsoral pathogens |
| spellingShingle | Pune N. Paqué Christopher Herz Joël S. Jenzer Daniel B. Wiedemeier Thomas Attin Nagihan Bostanci Georgios N. Belibasakis Kai Bao Philipp Körner Tanja Fritz Julia Prinz Patrick R. Schmidlin Thomas Thurnheer Florian J. Wegehaupt Konstantinos Mitsakakis Johannes R. Peham Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay Journal of Clinical Medicine point of care caries periodontitis oral health saliva diagnostics oral pathogens |
| title | Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay |
| title_full | Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay |
| title_fullStr | Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay |
| title_full_unstemmed | Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay |
| title_short | Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay |
| title_sort | microbial analysis of saliva to identify oral diseases using a point of care compatible qpcr assay |
| topic | point of care caries periodontitis oral health saliva diagnostics oral pathogens |
| url | https://www.mdpi.com/2077-0383/9/9/2945 |
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