Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells
Abstract Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients will increase the risk of zoonotic disease transmission. Human platelet lysate (HPL) is a potential surrogate and used in human cell expansion with rel...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-022-01387-9 |
| _version_ | 1798005815772184576 |
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| author | Tong Lei Yanyan Liu Shiwen Deng Zhuangzhuang Xiao Yanjie Yang Xiaoshuang Zhang Wangyu Bi Hongwu Du |
| author_facet | Tong Lei Yanyan Liu Shiwen Deng Zhuangzhuang Xiao Yanjie Yang Xiaoshuang Zhang Wangyu Bi Hongwu Du |
| author_sort | Tong Lei |
| collection | DOAJ |
| description | Abstract Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients will increase the risk of zoonotic disease transmission. Human platelet lysate (HPL) is a potential surrogate and used in human cell expansion with reliability in clinical applications. In this study, we synthesized chitosan/gelatin/gellan gum hydrogel supplemented with HPL and investigated the effect of 3D culture for SHED. TMT-tagged proteomics was used to decipher the secretome protein profiles of SHEDs and a total of 3209 proteins were identified, of which 23 were up-regulated and 192 were down-regulated. The results showed that hydrogel supplemented with HPL promoted SHED proliferation. After induction, the hydrogel coating contributed to osteogenic differentiation, adipogenic differentiation and differentiation into neural-like cells of SHED. SHED encapsulated in a hydrogel promotes migration and angiogenesis of HUVEC. In conclusion, our research found that hydrogel supplemented with HPL can be used as a method for SHED in standardized production and can contribute to the clinical application of SHED in cell therapy. |
| first_indexed | 2024-04-11T12:45:02Z |
| format | Article |
| id | doaj.art-49635f79e6b94ae595753b0766e7fc45 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T12:45:02Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-49635f79e6b94ae595753b0766e7fc452022-12-22T04:23:23ZengBMCJournal of Nanobiotechnology1477-31552022-04-0120111410.1186/s12951-022-01387-9Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cellsTong Lei0Yanyan Liu1Shiwen Deng2Zhuangzhuang Xiao3Yanjie Yang4Xiaoshuang Zhang5Wangyu Bi6Hongwu Du7School of Chemistry and Biological Engineering, University of Science and Technology BeijingKangyanbao Stem Cell (Beijing) Co., LtdDaxing Research Institute, University of Science and Technology BeijingSchool of Chemistry and Biological Engineering, University of Science and Technology BeijingSchool of Chemistry and Biological Engineering, University of Science and Technology BeijingSchool of Chemistry and Biological Engineering, University of Science and Technology BeijingSchool of Chemistry and Biological Engineering, University of Science and Technology BeijingSchool of Chemistry and Biological Engineering, University of Science and Technology BeijingAbstract Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients will increase the risk of zoonotic disease transmission. Human platelet lysate (HPL) is a potential surrogate and used in human cell expansion with reliability in clinical applications. In this study, we synthesized chitosan/gelatin/gellan gum hydrogel supplemented with HPL and investigated the effect of 3D culture for SHED. TMT-tagged proteomics was used to decipher the secretome protein profiles of SHEDs and a total of 3209 proteins were identified, of which 23 were up-regulated and 192 were down-regulated. The results showed that hydrogel supplemented with HPL promoted SHED proliferation. After induction, the hydrogel coating contributed to osteogenic differentiation, adipogenic differentiation and differentiation into neural-like cells of SHED. SHED encapsulated in a hydrogel promotes migration and angiogenesis of HUVEC. In conclusion, our research found that hydrogel supplemented with HPL can be used as a method for SHED in standardized production and can contribute to the clinical application of SHED in cell therapy.https://doi.org/10.1186/s12951-022-01387-9HPLSHEDSecretomeOsteogenesisAngiogenesisNeural-like cells |
| spellingShingle | Tong Lei Yanyan Liu Shiwen Deng Zhuangzhuang Xiao Yanjie Yang Xiaoshuang Zhang Wangyu Bi Hongwu Du Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells Journal of Nanobiotechnology HPL SHED Secretome Osteogenesis Angiogenesis Neural-like cells |
| title | Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells |
| title_full | Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells |
| title_fullStr | Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells |
| title_full_unstemmed | Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells |
| title_short | Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells |
| title_sort | hydrogel supplemented with human platelet lysate enhances multi lineage differentiation of mesenchymal stem cells |
| topic | HPL SHED Secretome Osteogenesis Angiogenesis Neural-like cells |
| url | https://doi.org/10.1186/s12951-022-01387-9 |
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