Ante- and post-mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic O3 dose
After reaching phytotoxic levels during the last century, tropospheric ozone (O3) pollution is likely to remain a major concern in the coming decades. Despite similar injury processes, there is astounding interspecific–and sometimes intraspecific–foliar symptom variability, which may be related to s...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977006/?tool=EBI |
| _version_ | 1811160088643108864 |
|---|---|
| author | Benjamin Turc Yves Jolivet Mireille Cabané Marcus Schaub Pierre Vollenweider |
| author_facet | Benjamin Turc Yves Jolivet Mireille Cabané Marcus Schaub Pierre Vollenweider |
| author_sort | Benjamin Turc |
| collection | DOAJ |
| description | After reaching phytotoxic levels during the last century, tropospheric ozone (O3) pollution is likely to remain a major concern in the coming decades. Despite similar injury processes, there is astounding interspecific–and sometimes intraspecific–foliar symptom variability, which may be related to spatial and temporal variation in injury dynamics. After characterizing the dynamics of physiological responses and O3 injury in the foliage of hybrid poplar in an earlier study, here we investigated the dynamics of changes in the cell structure occurring in the mesophyll as a function of O3 treatment, time, phytotoxic O3 dose (POD0), leaf developmental stage, and mesophyll layer. While the number of Hypersensitive Response-like (HR-like) lesions increased with higher O3 concentrations and POD0, especially in older leaves, most structural HR-like markers developed after cell death, independent of the experimental factors. The pace of degenerative Accelerated Cell Senescence (ACS) responses depended closely on the O3 concentration and POD0, in interaction with leaf age. Changes in total chlorophyll content, plastoglobuli and chloroplast shape pointed to thylakoid membranes in chloroplasts as being especially sensitive to O3 stress. Hence, our study demonstrates that early HR-like markers can provide reasonably specific, sensitive and reliable quantitative structural estimates of O3 stress for e.g. risk assessment studies, especially if they are associated with degenerative and thylakoid-related injury in chloroplasts from mesophyll. |
| first_indexed | 2024-04-10T05:52:47Z |
| format | Article |
| id | doaj.art-4965d158e90044fd9fe1502974fa28e9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T05:52:47Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4965d158e90044fd9fe1502974fa28e92023-03-04T05:31:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01183Ante- and post-mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic O3 doseBenjamin TurcYves JolivetMireille CabanéMarcus SchaubPierre VollenweiderAfter reaching phytotoxic levels during the last century, tropospheric ozone (O3) pollution is likely to remain a major concern in the coming decades. Despite similar injury processes, there is astounding interspecific–and sometimes intraspecific–foliar symptom variability, which may be related to spatial and temporal variation in injury dynamics. After characterizing the dynamics of physiological responses and O3 injury in the foliage of hybrid poplar in an earlier study, here we investigated the dynamics of changes in the cell structure occurring in the mesophyll as a function of O3 treatment, time, phytotoxic O3 dose (POD0), leaf developmental stage, and mesophyll layer. While the number of Hypersensitive Response-like (HR-like) lesions increased with higher O3 concentrations and POD0, especially in older leaves, most structural HR-like markers developed after cell death, independent of the experimental factors. The pace of degenerative Accelerated Cell Senescence (ACS) responses depended closely on the O3 concentration and POD0, in interaction with leaf age. Changes in total chlorophyll content, plastoglobuli and chloroplast shape pointed to thylakoid membranes in chloroplasts as being especially sensitive to O3 stress. Hence, our study demonstrates that early HR-like markers can provide reasonably specific, sensitive and reliable quantitative structural estimates of O3 stress for e.g. risk assessment studies, especially if they are associated with degenerative and thylakoid-related injury in chloroplasts from mesophyll.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977006/?tool=EBI |
| spellingShingle | Benjamin Turc Yves Jolivet Mireille Cabané Marcus Schaub Pierre Vollenweider Ante- and post-mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic O3 dose PLoS ONE |
| title | Ante- and post-mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic O3 dose |
| title_full | Ante- and post-mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic O3 dose |
| title_fullStr | Ante- and post-mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic O3 dose |
| title_full_unstemmed | Ante- and post-mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic O3 dose |
| title_short | Ante- and post-mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic O3 dose |
| title_sort | ante and post mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic o3 dose |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977006/?tool=EBI |
| work_keys_str_mv | AT benjaminturc anteandpostmortemcellularinjurydynamicsinhybridpoplarfoliageasafunctionofphytotoxico3dose AT yvesjolivet anteandpostmortemcellularinjurydynamicsinhybridpoplarfoliageasafunctionofphytotoxico3dose AT mireillecabane anteandpostmortemcellularinjurydynamicsinhybridpoplarfoliageasafunctionofphytotoxico3dose AT marcusschaub anteandpostmortemcellularinjurydynamicsinhybridpoplarfoliageasafunctionofphytotoxico3dose AT pierrevollenweider anteandpostmortemcellularinjurydynamicsinhybridpoplarfoliageasafunctionofphytotoxico3dose |