Changes Occurring on the Activity of Salivary Alpha-Amylase Proteoforms in Two Naturalistic Situations Using a Spectrophotometric Assay
This study aimed to evaluate the changes in the activity of total salivary alpha-amylase (TsAA) and both the non-glycosylated and glycosylated salivary alpha-amylase proteoforms (NGsAA and GsAA, respectively) in physical and psychological stress models, estimated using a simple and easily set-up met...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/10/3/227 |
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| author | María D. Contreras-Aguilar Sandra V. Mateo Fernando Tecles Christophe Hirtz Damián Escribano Jose J. Cerón |
| author_facet | María D. Contreras-Aguilar Sandra V. Mateo Fernando Tecles Christophe Hirtz Damián Escribano Jose J. Cerón |
| author_sort | María D. Contreras-Aguilar |
| collection | DOAJ |
| description | This study aimed to evaluate the changes in the activity of total salivary alpha-amylase (TsAA) and both the non-glycosylated and glycosylated salivary alpha-amylase proteoforms (NGsAA and GsAA, respectively) in physical and psychological stress models, estimated using a simple and easily set-up method. The method used was a spectrophotometric assay with 2-chloro-4-nitrophenyl-α-D-maltotriose (CNPG3) as a substrate, incubated with Concanavalin A (ConA) to remove most of the glycosylated protein from the sample. This method allowed the measurement of TsAA and estimation of NGsAA and GsAA activities with imprecision lower than 10%. When this method was applied to two different stress models, differences in the responses of the proteoforms were observed, with the NGsAA activity showing changes of higher magnitude after stress induction than the GsAA activity, and the highest correlation with the State–Trait Anxiety Inventory Scale in the Trier Social Stress Test (TSST). In conclusion, the activity of the two main sAA proteoforms can be easily estimated in saliva, and their measurement can provide additional information on TsAA activity in physical or psychological stress situations. |
| first_indexed | 2024-03-10T13:12:27Z |
| format | Article |
| id | doaj.art-4968dbd945374d79b1c380f72312f8d1 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T13:12:27Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-4968dbd945374d79b1c380f72312f8d12023-11-21T10:38:05ZengMDPI AGBiology2079-77372021-03-0110322710.3390/biology10030227Changes Occurring on the Activity of Salivary Alpha-Amylase Proteoforms in Two Naturalistic Situations Using a Spectrophotometric AssayMaría D. Contreras-Aguilar0Sandra V. Mateo1Fernando Tecles2Christophe Hirtz3Damián Escribano4Jose J. Cerón5Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100 Murcia, SpainInterdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100 Murcia, SpainInterdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100 Murcia, SpainIRMB-PPC, CHU Montpellier, Montpellier University, 34295 Montpellier, FranceInterdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100 Murcia, SpainInterdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100 Murcia, SpainThis study aimed to evaluate the changes in the activity of total salivary alpha-amylase (TsAA) and both the non-glycosylated and glycosylated salivary alpha-amylase proteoforms (NGsAA and GsAA, respectively) in physical and psychological stress models, estimated using a simple and easily set-up method. The method used was a spectrophotometric assay with 2-chloro-4-nitrophenyl-α-D-maltotriose (CNPG3) as a substrate, incubated with Concanavalin A (ConA) to remove most of the glycosylated protein from the sample. This method allowed the measurement of TsAA and estimation of NGsAA and GsAA activities with imprecision lower than 10%. When this method was applied to two different stress models, differences in the responses of the proteoforms were observed, with the NGsAA activity showing changes of higher magnitude after stress induction than the GsAA activity, and the highest correlation with the State–Trait Anxiety Inventory Scale in the Trier Social Stress Test (TSST). In conclusion, the activity of the two main sAA proteoforms can be easily estimated in saliva, and their measurement can provide additional information on TsAA activity in physical or psychological stress situations.https://www.mdpi.com/2079-7737/10/3/227salivary alpha-amylaseproteoformsglycoprotein depletionphysical effortpsychological challenge |
| spellingShingle | María D. Contreras-Aguilar Sandra V. Mateo Fernando Tecles Christophe Hirtz Damián Escribano Jose J. Cerón Changes Occurring on the Activity of Salivary Alpha-Amylase Proteoforms in Two Naturalistic Situations Using a Spectrophotometric Assay Biology salivary alpha-amylase proteoforms glycoprotein depletion physical effort psychological challenge |
| title | Changes Occurring on the Activity of Salivary Alpha-Amylase Proteoforms in Two Naturalistic Situations Using a Spectrophotometric Assay |
| title_full | Changes Occurring on the Activity of Salivary Alpha-Amylase Proteoforms in Two Naturalistic Situations Using a Spectrophotometric Assay |
| title_fullStr | Changes Occurring on the Activity of Salivary Alpha-Amylase Proteoforms in Two Naturalistic Situations Using a Spectrophotometric Assay |
| title_full_unstemmed | Changes Occurring on the Activity of Salivary Alpha-Amylase Proteoforms in Two Naturalistic Situations Using a Spectrophotometric Assay |
| title_short | Changes Occurring on the Activity of Salivary Alpha-Amylase Proteoforms in Two Naturalistic Situations Using a Spectrophotometric Assay |
| title_sort | changes occurring on the activity of salivary alpha amylase proteoforms in two naturalistic situations using a spectrophotometric assay |
| topic | salivary alpha-amylase proteoforms glycoprotein depletion physical effort psychological challenge |
| url | https://www.mdpi.com/2079-7737/10/3/227 |
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