Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemomitans</i>
<i>Aggregatibacter actinomycetemcomitans</i>, a Gram-negative bacterium with seven serotypes (a–g) according to the structure of its LPS, has been defined as one of the most important pathogens in the development of a dysbiotic periodontal biofilm and the onset of periodontitis (an infla...
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MDPI AG
2022-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1384 |
| _version_ | 1797433244760670208 |
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| author | Daniel Betancur Camila Muñoz Angel Oñate |
| author_facet | Daniel Betancur Camila Muñoz Angel Oñate |
| author_sort | Daniel Betancur |
| collection | DOAJ |
| description | <i>Aggregatibacter actinomycetemcomitans</i>, a Gram-negative bacterium with seven serotypes (a–g) according to the structure of its LPS, has been defined as one of the most important pathogens in the development of a dysbiotic periodontal biofilm and the onset of periodontitis (an inflammatory chronic disease of the tissues around the teeth), where the serotype b is characterized as the most virulent compared with the other serotypes. The aim of this study was to evaluate the expression of the macrophage polarization markers M0, M1, and M2 against <i>A. actinomycetemcomitans</i>. Methods: THP-1 cells were infected with <i>A. actinomycetemcomitans</i> serotypes a, b, and c. The expression of CD11b, CD4, CD14, and CD68 for M0; IL-6, HLA/DRA, and CXCL10 for M21, and IL-10, CD163, fibronectin-1 or FN1, and CCL17 was evaluated by qPCR at 2 and 24 h after infection. Results: An increase in the expression of these molecules was induced by all serotypes at both times of infection, showing higher levels of expression to the M1 panel at 2 and 24 h compared to other markers. Conclusions: <i>A. actinomycetemcomitans</i> has a role in the macrophage polarization to the M1 phenotype in a non-serotype-dependent manner. |
| first_indexed | 2024-03-09T10:14:16Z |
| format | Article |
| id | doaj.art-496b5f6a23b348c09b007961286492d7 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T10:14:16Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-496b5f6a23b348c09b007961286492d72023-12-01T22:28:31ZengMDPI AGMicroorganisms2076-26072022-07-01107138410.3390/microorganisms10071384Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemomitans</i>Daniel Betancur0Camila Muñoz1Angel Oñate2Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4030000, ChileLaboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4030000, ChileLaboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4030000, Chile<i>Aggregatibacter actinomycetemcomitans</i>, a Gram-negative bacterium with seven serotypes (a–g) according to the structure of its LPS, has been defined as one of the most important pathogens in the development of a dysbiotic periodontal biofilm and the onset of periodontitis (an inflammatory chronic disease of the tissues around the teeth), where the serotype b is characterized as the most virulent compared with the other serotypes. The aim of this study was to evaluate the expression of the macrophage polarization markers M0, M1, and M2 against <i>A. actinomycetemcomitans</i>. Methods: THP-1 cells were infected with <i>A. actinomycetemcomitans</i> serotypes a, b, and c. The expression of CD11b, CD4, CD14, and CD68 for M0; IL-6, HLA/DRA, and CXCL10 for M21, and IL-10, CD163, fibronectin-1 or FN1, and CCL17 was evaluated by qPCR at 2 and 24 h after infection. Results: An increase in the expression of these molecules was induced by all serotypes at both times of infection, showing higher levels of expression to the M1 panel at 2 and 24 h compared to other markers. Conclusions: <i>A. actinomycetemcomitans</i> has a role in the macrophage polarization to the M1 phenotype in a non-serotype-dependent manner.https://www.mdpi.com/2076-2607/10/7/1384inflammationperiodontal cellsperiodontal pathogensperiodontitisinnate immunityintracellular bacteria |
| spellingShingle | Daniel Betancur Camila Muñoz Angel Oñate Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemomitans</i> Microorganisms inflammation periodontal cells periodontal pathogens periodontitis innate immunity intracellular bacteria |
| title | Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemomitans</i> |
| title_full | Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemomitans</i> |
| title_fullStr | Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemomitans</i> |
| title_full_unstemmed | Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemomitans</i> |
| title_short | Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemomitans</i> |
| title_sort | expression of macrophage polarization markers against the most prevalent serotypes of i aggregatibacter actinomycetemomitans i |
| topic | inflammation periodontal cells periodontal pathogens periodontitis innate immunity intracellular bacteria |
| url | https://www.mdpi.com/2076-2607/10/7/1384 |
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