Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf Symptoms
Colletotrichum fructicola infects pear leaves, resulting in two major symptoms: tiny black spots (TS) followed by severe early defoliation and big necrotic lesions (BnL) without apparent damage depending on the pathotypes. How the same fungal species causes different symptoms remains unclear. To und...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-02-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.761133/full |
| _version_ | 1819277552416456704 |
|---|---|
| author | Min Fu Min Fu Min Fu Min Fu Min Fu Qing Bai Hui Zhang Yashuang Guo Yuhong Peng Pengfei Zhang Liang Shen Ni Hong Ni Hong Ni Hong Ni Hong Wenxing Xu Wenxing Xu Wenxing Xu Wenxing Xu Wenxing Xu Guoping Wang Guoping Wang Guoping Wang Guoping Wang |
| author_facet | Min Fu Min Fu Min Fu Min Fu Min Fu Qing Bai Hui Zhang Yashuang Guo Yuhong Peng Pengfei Zhang Liang Shen Ni Hong Ni Hong Ni Hong Ni Hong Wenxing Xu Wenxing Xu Wenxing Xu Wenxing Xu Wenxing Xu Guoping Wang Guoping Wang Guoping Wang Guoping Wang |
| author_sort | Min Fu |
| collection | DOAJ |
| description | Colletotrichum fructicola infects pear leaves, resulting in two major symptoms: tiny black spots (TS) followed by severe early defoliation and big necrotic lesions (BnL) without apparent damage depending on the pathotypes. How the same fungal species causes different symptoms remains unclear. To understand the molecular mechanism underlying the resulting diseases and the diverse symptoms, two C. fructicola pathogenetic strains (PAFQ31 and PAFQ32 responsible for TS and BnL symptoms, respectively) were inoculated on Pyrus pyrifolia leaves and subjected to transcriptome sequencing at the quiescent stage (QS) and necrotrophic stage (NS), respectively. In planta, the genes involved in the salicylic acid (SA) signaling pathway were upregulated at the NS caused by the infection of each strain. In contrast, the ethylene (ET), abscisic acid (ABA), and jasmonic acid (JA) signaling pathways were specifically related to the TS symptoms caused by the infection of strain PAFQ31, corresponding to the yellowish and early defoliation symptoms triggered by the strain infection. Correspondingly, SA was accumulated in similar levels in the leaves infected by each strain at NS, but JA was significantly higher in the PAFQ31-infected as measured using high-performance liquid chromatography. Weighted gene co-expression network analysis also reveals specific genes, pathways, phytohormones, and transcription factors (TFs) associated with the PAFQ31-associated early defoliation. Taken together, these data suggest that specific metabolic pathways were regulated in P. pyrifolia in response to the infection of two C. fructicola pathotypes resulting in the diverse symptoms: JA, ET, and ABA accumulated in the PAFQ31-infected leaves, which negatively affected the chlorophyll metabolism and photosynthesis pathways while positively affecting the expression of senescence-associated TFs and genes, resulted in leaf yellowing and defoliation; whereas SA inhibited JA-induced gene expression in the PAFQ32-infected leaves, which led to hypersensitive response-like reaction and BnL symptoms. |
| first_indexed | 2024-12-23T23:57:56Z |
| format | Article |
| id | doaj.art-496c8ca1f9d54a728b21e1ce4e957e6b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T23:57:56Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-496c8ca1f9d54a728b21e1ce4e957e6b2022-12-21T17:25:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-02-011310.3389/fpls.2022.761133761133Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf SymptomsMin Fu0Min Fu1Min Fu2Min Fu3Min Fu4Qing Bai5Hui Zhang6Yashuang Guo7Yuhong Peng8Pengfei Zhang9Liang Shen10Ni Hong11Ni Hong12Ni Hong13Ni Hong14Wenxing Xu15Wenxing Xu16Wenxing Xu17Wenxing Xu18Wenxing Xu19Guoping Wang20Guoping Wang21Guoping Wang22Guoping Wang23Hubei Hongshan Laboratory, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Wuhan, ChinaKey Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Wuhan, ChinaKey Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaHubei Hongshan Laboratory, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Wuhan, ChinaKey Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Wuhan, ChinaKey Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaColletotrichum fructicola infects pear leaves, resulting in two major symptoms: tiny black spots (TS) followed by severe early defoliation and big necrotic lesions (BnL) without apparent damage depending on the pathotypes. How the same fungal species causes different symptoms remains unclear. To understand the molecular mechanism underlying the resulting diseases and the diverse symptoms, two C. fructicola pathogenetic strains (PAFQ31 and PAFQ32 responsible for TS and BnL symptoms, respectively) were inoculated on Pyrus pyrifolia leaves and subjected to transcriptome sequencing at the quiescent stage (QS) and necrotrophic stage (NS), respectively. In planta, the genes involved in the salicylic acid (SA) signaling pathway were upregulated at the NS caused by the infection of each strain. In contrast, the ethylene (ET), abscisic acid (ABA), and jasmonic acid (JA) signaling pathways were specifically related to the TS symptoms caused by the infection of strain PAFQ31, corresponding to the yellowish and early defoliation symptoms triggered by the strain infection. Correspondingly, SA was accumulated in similar levels in the leaves infected by each strain at NS, but JA was significantly higher in the PAFQ31-infected as measured using high-performance liquid chromatography. Weighted gene co-expression network analysis also reveals specific genes, pathways, phytohormones, and transcription factors (TFs) associated with the PAFQ31-associated early defoliation. Taken together, these data suggest that specific metabolic pathways were regulated in P. pyrifolia in response to the infection of two C. fructicola pathotypes resulting in the diverse symptoms: JA, ET, and ABA accumulated in the PAFQ31-infected leaves, which negatively affected the chlorophyll metabolism and photosynthesis pathways while positively affecting the expression of senescence-associated TFs and genes, resulted in leaf yellowing and defoliation; whereas SA inhibited JA-induced gene expression in the PAFQ32-infected leaves, which led to hypersensitive response-like reaction and BnL symptoms.https://www.frontiersin.org/articles/10.3389/fpls.2022.761133/fullpearColletotrichum fructicolaearly defoliationtranscriptome sequencingphytohormone |
| spellingShingle | Min Fu Min Fu Min Fu Min Fu Min Fu Qing Bai Hui Zhang Yashuang Guo Yuhong Peng Pengfei Zhang Liang Shen Ni Hong Ni Hong Ni Hong Ni Hong Wenxing Xu Wenxing Xu Wenxing Xu Wenxing Xu Wenxing Xu Guoping Wang Guoping Wang Guoping Wang Guoping Wang Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf Symptoms Frontiers in Plant Science pear Colletotrichum fructicola early defoliation transcriptome sequencing phytohormone |
| title | Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf Symptoms |
| title_full | Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf Symptoms |
| title_fullStr | Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf Symptoms |
| title_full_unstemmed | Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf Symptoms |
| title_short | Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf Symptoms |
| title_sort | transcriptome analysis of the molecular patterns of pear plants infected by two colletotrichum fructicola pathogenic strains causing contrasting sets of leaf symptoms |
| topic | pear Colletotrichum fructicola early defoliation transcriptome sequencing phytohormone |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.761133/full |
| work_keys_str_mv | AT minfu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT minfu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT minfu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT minfu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT minfu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT qingbai transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT huizhang transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT yashuangguo transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT yuhongpeng transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT pengfeizhang transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT liangshen transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT nihong transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT nihong transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT nihong transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT nihong transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT wenxingxu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT wenxingxu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT wenxingxu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT wenxingxu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT wenxingxu transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT guopingwang transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT guopingwang transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT guopingwang transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms AT guopingwang transcriptomeanalysisofthemolecularpatternsofpearplantsinfectedbytwocolletotrichumfructicolapathogenicstrainscausingcontrastingsetsofleafsymptoms |