The Metabolism of a Novel Cytochrome P450 (<i>CYP77B34</i>) in Tribenuron-Methyl-Resistant <i>Descurainia sophia</i> L. to Herbicides with Different Mode of Actions
<i>Descurainia sophia</i> L. (flixweeds) is a noxious broad-leaf weed infesting winter wheat fields in China that has evolved high resistance to tribenuron-methyl. In this work, a brand new gene <i>CYP77B34</i> was cloned from tribenuron-methyl-resistant (TR) <i>D. soph...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/10/5812 |
| Summary: | <i>Descurainia sophia</i> L. (flixweeds) is a noxious broad-leaf weed infesting winter wheat fields in China that has evolved high resistance to tribenuron-methyl. In this work, a brand new gene <i>CYP77B34</i> was cloned from tribenuron-methyl-resistant (TR) <i>D. sophia</i> and transferred into <i>Arabidopsis thaliana</i>, and the sensitivities of <i>Arabidopsis</i> with or without the <i>CYP77B34</i> transgene to herbicides with a different mode of actions (MoAs) were tested. Compared to <i>Arabidopsis</i> expressing pCAMBIA1302-GFP (empty plasmid), <i>Arabidopsis</i> transferring pCAMBIA1302-CYP77B34 (recombinant plasmid) became resistant to acetolactate synthase (ALS)-inhibiting herbicide tribenuron-methyl, protoporphyrinogen oxidase (PPO)-inhibiting herbicides carfentrazone-ethyl and oxyfluorfen. Cytochrome P450 inhibitor malathion could reverse the resistance to tribenuron-methyl, carfentrazone-ethyl and oxyfluorfen in transgenic <i>Arabidopsis</i> plants. In addition, the metabolic rates of tribenuron-methyl in <i>Arabidopsis</i> expressing <i>CYP77B34</i> were significantly higher than those in <i>Arabidopsis</i> expressing pCAMBIA1302-GFP. Other than that, the transgenic plants showed some tolerance to very-long-chain fatty acid synthesis (VLCFAs)-inhibiting herbicide pretilachlor and photosystem (PS) II-inhibiting herbicide bromoxynil. Subcellular localization revealed that the CYP77B34 protein was located in the endoplasmic reticulum (ER). These results clearly indicated that <i>CYP77B34</i> mediated <i>D. sophia</i> resistance to tribenuron-methyl and may have been involved in <i>D. sophia</i> cross-resistance to carfentrazone-ethyl, oxyfluorfen, pretilachlor and bromoxynil. |
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| ISSN: | 1661-6596 1422-0067 |