The Metabolism of a Novel Cytochrome P450 (<i>CYP77B34</i>) in Tribenuron-Methyl-Resistant <i>Descurainia sophia</i> L. to Herbicides with Different Mode of Actions
<i>Descurainia sophia</i> L. (flixweeds) is a noxious broad-leaf weed infesting winter wheat fields in China that has evolved high resistance to tribenuron-methyl. In this work, a brand new gene <i>CYP77B34</i> was cloned from tribenuron-methyl-resistant (TR) <i>D. soph...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/10/5812 |
| _version_ | 1797499083989975040 |
|---|---|
| author | Jing Shen Qian Yang Lubo Hao Lingling Zhang Xuefeng Li Mingqi Zheng |
| author_facet | Jing Shen Qian Yang Lubo Hao Lingling Zhang Xuefeng Li Mingqi Zheng |
| author_sort | Jing Shen |
| collection | DOAJ |
| description | <i>Descurainia sophia</i> L. (flixweeds) is a noxious broad-leaf weed infesting winter wheat fields in China that has evolved high resistance to tribenuron-methyl. In this work, a brand new gene <i>CYP77B34</i> was cloned from tribenuron-methyl-resistant (TR) <i>D. sophia</i> and transferred into <i>Arabidopsis thaliana</i>, and the sensitivities of <i>Arabidopsis</i> with or without the <i>CYP77B34</i> transgene to herbicides with a different mode of actions (MoAs) were tested. Compared to <i>Arabidopsis</i> expressing pCAMBIA1302-GFP (empty plasmid), <i>Arabidopsis</i> transferring pCAMBIA1302-CYP77B34 (recombinant plasmid) became resistant to acetolactate synthase (ALS)-inhibiting herbicide tribenuron-methyl, protoporphyrinogen oxidase (PPO)-inhibiting herbicides carfentrazone-ethyl and oxyfluorfen. Cytochrome P450 inhibitor malathion could reverse the resistance to tribenuron-methyl, carfentrazone-ethyl and oxyfluorfen in transgenic <i>Arabidopsis</i> plants. In addition, the metabolic rates of tribenuron-methyl in <i>Arabidopsis</i> expressing <i>CYP77B34</i> were significantly higher than those in <i>Arabidopsis</i> expressing pCAMBIA1302-GFP. Other than that, the transgenic plants showed some tolerance to very-long-chain fatty acid synthesis (VLCFAs)-inhibiting herbicide pretilachlor and photosystem (PS) II-inhibiting herbicide bromoxynil. Subcellular localization revealed that the CYP77B34 protein was located in the endoplasmic reticulum (ER). These results clearly indicated that <i>CYP77B34</i> mediated <i>D. sophia</i> resistance to tribenuron-methyl and may have been involved in <i>D. sophia</i> cross-resistance to carfentrazone-ethyl, oxyfluorfen, pretilachlor and bromoxynil. |
| first_indexed | 2024-03-10T03:42:22Z |
| format | Article |
| id | doaj.art-496e596fbaad4098bb283bd83c05b3ed |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:42:22Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-496e596fbaad4098bb283bd83c05b3ed2023-11-23T11:28:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310581210.3390/ijms23105812The Metabolism of a Novel Cytochrome P450 (<i>CYP77B34</i>) in Tribenuron-Methyl-Resistant <i>Descurainia sophia</i> L. to Herbicides with Different Mode of ActionsJing Shen0Qian Yang1Lubo Hao2Lingling Zhang3Xuefeng Li4Mingqi Zheng5Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, ChinaDepartment of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, ChinaDepartment of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, ChinaDepartment of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, ChinaDepartment of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, ChinaDepartment of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China<i>Descurainia sophia</i> L. (flixweeds) is a noxious broad-leaf weed infesting winter wheat fields in China that has evolved high resistance to tribenuron-methyl. In this work, a brand new gene <i>CYP77B34</i> was cloned from tribenuron-methyl-resistant (TR) <i>D. sophia</i> and transferred into <i>Arabidopsis thaliana</i>, and the sensitivities of <i>Arabidopsis</i> with or without the <i>CYP77B34</i> transgene to herbicides with a different mode of actions (MoAs) were tested. Compared to <i>Arabidopsis</i> expressing pCAMBIA1302-GFP (empty plasmid), <i>Arabidopsis</i> transferring pCAMBIA1302-CYP77B34 (recombinant plasmid) became resistant to acetolactate synthase (ALS)-inhibiting herbicide tribenuron-methyl, protoporphyrinogen oxidase (PPO)-inhibiting herbicides carfentrazone-ethyl and oxyfluorfen. Cytochrome P450 inhibitor malathion could reverse the resistance to tribenuron-methyl, carfentrazone-ethyl and oxyfluorfen in transgenic <i>Arabidopsis</i> plants. In addition, the metabolic rates of tribenuron-methyl in <i>Arabidopsis</i> expressing <i>CYP77B34</i> were significantly higher than those in <i>Arabidopsis</i> expressing pCAMBIA1302-GFP. Other than that, the transgenic plants showed some tolerance to very-long-chain fatty acid synthesis (VLCFAs)-inhibiting herbicide pretilachlor and photosystem (PS) II-inhibiting herbicide bromoxynil. Subcellular localization revealed that the CYP77B34 protein was located in the endoplasmic reticulum (ER). These results clearly indicated that <i>CYP77B34</i> mediated <i>D. sophia</i> resistance to tribenuron-methyl and may have been involved in <i>D. sophia</i> cross-resistance to carfentrazone-ethyl, oxyfluorfen, pretilachlor and bromoxynil.https://www.mdpi.com/1422-0067/23/10/5812flixweedcytochrome P450 monooxygenasesmetabolic resistanceresistance patterns |
| spellingShingle | Jing Shen Qian Yang Lubo Hao Lingling Zhang Xuefeng Li Mingqi Zheng The Metabolism of a Novel Cytochrome P450 (<i>CYP77B34</i>) in Tribenuron-Methyl-Resistant <i>Descurainia sophia</i> L. to Herbicides with Different Mode of Actions International Journal of Molecular Sciences flixweed cytochrome P450 monooxygenases metabolic resistance resistance patterns |
| title | The Metabolism of a Novel Cytochrome P450 (<i>CYP77B34</i>) in Tribenuron-Methyl-Resistant <i>Descurainia sophia</i> L. to Herbicides with Different Mode of Actions |
| title_full | The Metabolism of a Novel Cytochrome P450 (<i>CYP77B34</i>) in Tribenuron-Methyl-Resistant <i>Descurainia sophia</i> L. to Herbicides with Different Mode of Actions |
| title_fullStr | The Metabolism of a Novel Cytochrome P450 (<i>CYP77B34</i>) in Tribenuron-Methyl-Resistant <i>Descurainia sophia</i> L. to Herbicides with Different Mode of Actions |
| title_full_unstemmed | The Metabolism of a Novel Cytochrome P450 (<i>CYP77B34</i>) in Tribenuron-Methyl-Resistant <i>Descurainia sophia</i> L. to Herbicides with Different Mode of Actions |
| title_short | The Metabolism of a Novel Cytochrome P450 (<i>CYP77B34</i>) in Tribenuron-Methyl-Resistant <i>Descurainia sophia</i> L. to Herbicides with Different Mode of Actions |
| title_sort | metabolism of a novel cytochrome p450 i cyp77b34 i in tribenuron methyl resistant i descurainia sophia i l to herbicides with different mode of actions |
| topic | flixweed cytochrome P450 monooxygenases metabolic resistance resistance patterns |
| url | https://www.mdpi.com/1422-0067/23/10/5812 |
| work_keys_str_mv | AT jingshen themetabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT qianyang themetabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT lubohao themetabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT linglingzhang themetabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT xuefengli themetabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT mingqizheng themetabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT jingshen metabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT qianyang metabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT lubohao metabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT linglingzhang metabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT xuefengli metabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions AT mingqizheng metabolismofanovelcytochromep450icyp77b34iintribenuronmethylresistantidescurainiasophiailtoherbicideswithdifferentmodeofactions |