Strains and virulence genes of salmonella with multidrug resistance isolated from chicken carcasses (Hanoi, Vietnam)
Salmonella enterica is one of dangerous food-borne pathogens listed by the World Health Organization (WHO). In Vietnam, poultry is one of the most widely eaten meats and is reported as a common source of S. enterica contamination. The aim of this study was to examine multi-resistant Salmonella st...
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| Format: | Article |
| Language: | English |
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FBSI “Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”
2023-03-01
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| Series: | Analiz Riska Zdorovʹû |
| Subjects: | |
| Online Access: | https://journal.fcrisk.ru/eng/2023/1/11 |
| _version_ | 1797743778636759040 |
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| author | Xuan Da Pham Hao Le Thi Hong Huyen Tran Thi Thanh Long Thanh Le Hoa Vinh Le Ninh Hanh Thi Minh Le Tran Nguyen Thanh Trung |
| author_facet | Xuan Da Pham Hao Le Thi Hong Huyen Tran Thi Thanh Long Thanh Le Hoa Vinh Le Ninh Hanh Thi Minh Le Tran Nguyen Thanh Trung |
| author_sort | Xuan Da Pham |
| collection | DOAJ |
| description | Salmonella enterica is one of dangerous food-borne pathogens listed by the World Health Organization (WHO). In Vietnam, poultry is one of the most widely eaten meats and is reported as a common source of S. enterica contamination.
The aim of this study was to examine multi-resistant Salmonella strains, to identify susceptibility to antibiotics by using 15 different types of medications and to perform sequencing to analyze antibiotic resistance genes, genotypes, multi-locus sequence-based typing (MLST), and plasmids.
The result of the antibiotic susceptibility test indicated that phenotypic resistance to 9–11 types of antimicrobials was confirmed in all strains. Among 06 sequenced strains, we identified 43 genes associated with antibiotic resistance: strains carrying a range of genes that are associated with aminoglycoside resistance (aac(3), aac(6), ant(3), aph(3), aph(6), aadA); all strains carried blaCTX-M-55 or blaCTX-M-65 gene, which were resistant to the 3rd generation antibiotics; there were also frequently observed sul1, sul2, sul3, tet (A), qnrS1, floR, dfrA14 or dfrA27 genes in sequenced isolates. Besides, the genome sequencing also indicated that all strains carried pathogenicity islands SPI 1, SPI 2, and SPI 3 thereby creating many potential triggers of the disease. Additionally, some carried C63PI, SPI 9, SPI 13, SPI 14, and plus some plasmids such as Col156, IncHI2, IncHI2A, IncFIB, Col (MGD2).
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| first_indexed | 2024-03-12T15:00:20Z |
| format | Article |
| id | doaj.art-49709e492b554836963c9ef44f550cb7 |
| institution | Directory Open Access Journal |
| issn | 2308-1155 2308-1163 |
| language | English |
| last_indexed | 2024-03-12T15:00:20Z |
| publishDate | 2023-03-01 |
| publisher | FBSI “Federal Scientific Center for Medical and Preventive Health Risk Management Technologies” |
| record_format | Article |
| series | Analiz Riska Zdorovʹû |
| spelling | doaj.art-49709e492b554836963c9ef44f550cb72023-08-14T08:19:15ZengFBSI “Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”Analiz Riska Zdorovʹû2308-11552308-11632023-03-01110511310.21668/health.risk/2023.1.11.engStrains and virulence genes of salmonella with multidrug resistance isolated from chicken carcasses (Hanoi, Vietnam)Xuan Da Pham0Hao Le Thi Hong1Huyen Tran Thi Thanh2Long Thanh Le3Hoa Vinh Le4Ninh Hanh Thi5Minh Le Tran6Nguyen Thanh Trung7Vietnam National University, Ho Chi Minh City, VietnamNational Institute for Food Control, 65 Fam Tan Duat Str., Hanoi, VietnamVinmec Research Institute of Stem cell and Gene Technology, Hai Ba Trung, Hanoi, VietnamNational Institute for Food Control, 65 Fam Tan Duat Str., Hanoi, VietnamNational Institute for Food Control, 65 Fam Tan Duat Str., Hanoi, VietnamNational Institute for Food Control, 65 Fam Tan Duat Str., Hanoi, VietnamHigh School for Gifted Students, Hanoi University of Science, 182 Luong The Vinh Str., Hanoi, VietnamNational Institute for Food Control, 65 Fam Tan Duat Str., Hanoi, VietnamSalmonella enterica is one of dangerous food-borne pathogens listed by the World Health Organization (WHO). In Vietnam, poultry is one of the most widely eaten meats and is reported as a common source of S. enterica contamination. The aim of this study was to examine multi-resistant Salmonella strains, to identify susceptibility to antibiotics by using 15 different types of medications and to perform sequencing to analyze antibiotic resistance genes, genotypes, multi-locus sequence-based typing (MLST), and plasmids. The result of the antibiotic susceptibility test indicated that phenotypic resistance to 9–11 types of antimicrobials was confirmed in all strains. Among 06 sequenced strains, we identified 43 genes associated with antibiotic resistance: strains carrying a range of genes that are associated with aminoglycoside resistance (aac(3), aac(6), ant(3), aph(3), aph(6), aadA); all strains carried blaCTX-M-55 or blaCTX-M-65 gene, which were resistant to the 3rd generation antibiotics; there were also frequently observed sul1, sul2, sul3, tet (A), qnrS1, floR, dfrA14 or dfrA27 genes in sequenced isolates. Besides, the genome sequencing also indicated that all strains carried pathogenicity islands SPI 1, SPI 2, and SPI 3 thereby creating many potential triggers of the disease. Additionally, some carried C63PI, SPI 9, SPI 13, SPI 14, and plus some plasmids such as Col156, IncHI2, IncHI2A, IncFIB, Col (MGD2). https://journal.fcrisk.ru/eng/2023/1/11antimicrobialssalmonellamultidrug resistancevirulence factorplasmidchickenantibiotic resistance gensalmonella pathogenicity island (spi)beta-lactam |
| spellingShingle | Xuan Da Pham Hao Le Thi Hong Huyen Tran Thi Thanh Long Thanh Le Hoa Vinh Le Ninh Hanh Thi Minh Le Tran Nguyen Thanh Trung Strains and virulence genes of salmonella with multidrug resistance isolated from chicken carcasses (Hanoi, Vietnam) Analiz Riska Zdorovʹû antimicrobials salmonella multidrug resistance virulence factor plasmid chicken antibiotic resistance gen salmonella pathogenicity island (spi) beta-lactam |
| title | Strains and virulence genes of salmonella with multidrug resistance isolated from chicken carcasses (Hanoi, Vietnam) |
| title_full | Strains and virulence genes of salmonella with multidrug resistance isolated from chicken carcasses (Hanoi, Vietnam) |
| title_fullStr | Strains and virulence genes of salmonella with multidrug resistance isolated from chicken carcasses (Hanoi, Vietnam) |
| title_full_unstemmed | Strains and virulence genes of salmonella with multidrug resistance isolated from chicken carcasses (Hanoi, Vietnam) |
| title_short | Strains and virulence genes of salmonella with multidrug resistance isolated from chicken carcasses (Hanoi, Vietnam) |
| title_sort | strains and virulence genes of salmonella with multidrug resistance isolated from chicken carcasses hanoi vietnam |
| topic | antimicrobials salmonella multidrug resistance virulence factor plasmid chicken antibiotic resistance gen salmonella pathogenicity island (spi) beta-lactam |
| url | https://journal.fcrisk.ru/eng/2023/1/11 |
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