Evaluation of the antifungal, antibacterial, and anti-inflammatory activity of a halophyte plant Arthrocnemum macrostachyum Koch extracts.
Background: Indiscriminate use of conventional antibiotics has become a major factor responsible for the emergence and dissemination of multidrug-resistant strains of microorganisms. In this regard, we analyzed the plant extracts as an alternative solution for the inhibition of the resistant bugs....
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| Format: | Article |
| Language: | English |
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Advance Educational Institute & Research Centre
2021-12-01
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| Series: | International Journal of Endorsing Health Science Research |
| Subjects: | |
| Online Access: | http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/662/748 |
| _version_ | 1819236225054146560 |
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| author | Sana Eijaz Muneeba Khan Amber Mirza Anjum Parveen Shazia Mansuri Muhammad Nadir |
| author_facet | Sana Eijaz Muneeba Khan Amber Mirza Anjum Parveen Shazia Mansuri Muhammad Nadir |
| author_sort | Sana Eijaz |
| collection | DOAJ |
| description | Background: Indiscriminate use of conventional antibiotics has become a major factor responsible for the emergence and dissemination of multidrug-resistant strains of microorganisms. In this regard, we analyzed the plant extracts as an alternative solution for the inhibition of the resistant bugs.
Methodology: This study was planned to investigate the antifungal, antibacterial and anti-inflammatory activities of hexane, ethyl acetate, chloroform, and methanol extracts of Arthrocnemum macrostachyum. Soxhlet apparatus was used to prepare different extracts of the whole plant using hexane, ethyl acetate, chloroform, and methanol. The antifungal and antibacterial activity was analyzed with the help of a good diffusion technique using different concentrations of plant extracts. The anti-inflammatory activity was also checked by observing the inhibition of albumin denaturation.
Results: Maximum antifungal activity was observed by hexane, methanol, and chloroform extracts, and maximum antibacterial activity showed by the methanol extract. The most sensitive fungi found were Mucor, Aspergillus flavus, Saccharomyces, and Candida. Trichophyton mentagrophytes were found resistant to all the extracts. The most sensitive bacterial isolates found were Salmonella typhi, Proteus mirabilis, Pseudomonas aeruginosa, and Citrobacter freundii. The most resistant organisms were Streptococcus fecalis and Shigella dysenteriae. Methanol and chloroform extracts produced observable anti-inflammatory potential by inhibiting albumin denaturation. Statistical analysis was performed on SPSS version 23.0.
Conclusion: The plant extracts showed significant antifungal, antibacterial and anti-inflammatory activity. These findings may support using the Arthrocnemum macrostachyum whole plant as an anti-inflammatory, antibacterial, and traditional antifungal plant. Our results demonstrate that this plant has considerable therapeutic effects on potential infectious agents and can be used to alternative conventional medicines. |
| first_indexed | 2024-12-23T13:01:03Z |
| format | Article |
| id | doaj.art-49719ab7a6c84b26bc1c55b4086d6666 |
| institution | Directory Open Access Journal |
| issn | 2307-3748 2310-3841 |
| language | English |
| last_indexed | 2024-12-23T13:01:03Z |
| publishDate | 2021-12-01 |
| publisher | Advance Educational Institute & Research Centre |
| record_format | Article |
| series | International Journal of Endorsing Health Science Research |
| spelling | doaj.art-49719ab7a6c84b26bc1c55b4086d66662022-12-21T17:46:02ZengAdvance Educational Institute & Research CentreInternational Journal of Endorsing Health Science Research2307-37482310-38412021-12-0194499510https://doi.org/10.29052/IJEHSR.v9.i4.2021.499-510Evaluation of the antifungal, antibacterial, and anti-inflammatory activity of a halophyte plant Arthrocnemum macrostachyum Koch extracts.Sana Eijaz0https://orcid.org/0000-0002-1466-2932Muneeba Khan1https://orcid.org/0000-0003-2923-2430Amber Mirza2https://orcid.org/0000-0002-4731-1354Anjum Parveen3https://orcid.org/0000-0003-2533-1386Shazia Mansuri4https://orcid.org/0000-0002-7104-1868Muhammad Nadir5https://orcid.org/0000-0001-8451-222XDepartment of Microbiology, University of Karachi, Karachi-Pakistan.Center for Plant Conservation, University of Karachi, Karachi-Pakistan.Department of Microbiology, University of Karachi, Karachi-Pakistan.Center for Plant Conservation, University of Karachi, Karachi-Pakistan.Center for Plant Conservation, University of Karachi, Karachi-Pakistan.1. Department of Chemistry, University of Karachi, Karachi-Pakistan. 2. Department of Chemistry, Government Degree Boys College, Karachi-Pakistan.Background: Indiscriminate use of conventional antibiotics has become a major factor responsible for the emergence and dissemination of multidrug-resistant strains of microorganisms. In this regard, we analyzed the plant extracts as an alternative solution for the inhibition of the resistant bugs. Methodology: This study was planned to investigate the antifungal, antibacterial and anti-inflammatory activities of hexane, ethyl acetate, chloroform, and methanol extracts of Arthrocnemum macrostachyum. Soxhlet apparatus was used to prepare different extracts of the whole plant using hexane, ethyl acetate, chloroform, and methanol. The antifungal and antibacterial activity was analyzed with the help of a good diffusion technique using different concentrations of plant extracts. The anti-inflammatory activity was also checked by observing the inhibition of albumin denaturation. Results: Maximum antifungal activity was observed by hexane, methanol, and chloroform extracts, and maximum antibacterial activity showed by the methanol extract. The most sensitive fungi found were Mucor, Aspergillus flavus, Saccharomyces, and Candida. Trichophyton mentagrophytes were found resistant to all the extracts. The most sensitive bacterial isolates found were Salmonella typhi, Proteus mirabilis, Pseudomonas aeruginosa, and Citrobacter freundii. The most resistant organisms were Streptococcus fecalis and Shigella dysenteriae. Methanol and chloroform extracts produced observable anti-inflammatory potential by inhibiting albumin denaturation. Statistical analysis was performed on SPSS version 23.0. Conclusion: The plant extracts showed significant antifungal, antibacterial and anti-inflammatory activity. These findings may support using the Arthrocnemum macrostachyum whole plant as an anti-inflammatory, antibacterial, and traditional antifungal plant. Our results demonstrate that this plant has considerable therapeutic effects on potential infectious agents and can be used to alternative conventional medicines.http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/662/748arthrocnemum macrostachyumextractsanti-inflammatoryantifungalantibacterialwell-diffusion assay |
| spellingShingle | Sana Eijaz Muneeba Khan Amber Mirza Anjum Parveen Shazia Mansuri Muhammad Nadir Evaluation of the antifungal, antibacterial, and anti-inflammatory activity of a halophyte plant Arthrocnemum macrostachyum Koch extracts. International Journal of Endorsing Health Science Research arthrocnemum macrostachyum extracts anti-inflammatory antifungal antibacterial well-diffusion assay |
| title | Evaluation of the antifungal, antibacterial, and anti-inflammatory activity of a halophyte plant Arthrocnemum macrostachyum Koch extracts. |
| title_full | Evaluation of the antifungal, antibacterial, and anti-inflammatory activity of a halophyte plant Arthrocnemum macrostachyum Koch extracts. |
| title_fullStr | Evaluation of the antifungal, antibacterial, and anti-inflammatory activity of a halophyte plant Arthrocnemum macrostachyum Koch extracts. |
| title_full_unstemmed | Evaluation of the antifungal, antibacterial, and anti-inflammatory activity of a halophyte plant Arthrocnemum macrostachyum Koch extracts. |
| title_short | Evaluation of the antifungal, antibacterial, and anti-inflammatory activity of a halophyte plant Arthrocnemum macrostachyum Koch extracts. |
| title_sort | evaluation of the antifungal antibacterial and anti inflammatory activity of a halophyte plant arthrocnemum macrostachyum koch extracts |
| topic | arthrocnemum macrostachyum extracts anti-inflammatory antifungal antibacterial well-diffusion assay |
| url | http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/662/748 |
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