First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes
This study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S o...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2016-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100051&lng=en&tlng=en |
| _version_ | 1827847494196789248 |
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| author | Maria Helena Galdino Figueiredo-Carvalho Livia de Souza Ramos Leonardo Silva Barbedo Alessandra Leal da Silva Chaves Ilda Akemi Muramoto André Luis Souza dos Santos Rodrigo Almeida-Paes Rosely Maria Zancopé-Oliveira |
| author_facet | Maria Helena Galdino Figueiredo-Carvalho Livia de Souza Ramos Leonardo Silva Barbedo Alessandra Leal da Silva Chaves Ilda Akemi Muramoto André Luis Souza dos Santos Rodrigo Almeida-Paes Rosely Maria Zancopé-Oliveira |
| author_sort | Maria Helena Galdino Figueiredo-Carvalho |
| collection | DOAJ |
| description | This study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to fluconazole resistance. Adherence to inert surfaces was conducted onto glass and polystyrene. The biofilm formation and antifungal susceptibility on biofilm-growing cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal strains were able to bind both tested surfaces and form biofilm, with a binding preference to polystyrene (p < 0.001). AMB promoted significant reductions (≈50%) in biofilm production by our C. nivariensis strain using both methodologies. This reduction was also observed for CAS and POS, but only in the XTT assay. All strains were excellent protease producers and moderate phytase producers, but lipases were not detected. This study reinforces the pathogenic potential of C. nivariensis and its possible resistance profile to the azolic drugs generally used for candidiasis management. |
| first_indexed | 2024-03-12T09:31:05Z |
| format | Article |
| id | doaj.art-4971fbf1e31a468f8d449b447fc235be |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:31:05Z |
| publishDate | 2016-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-4971fbf1e31a468f8d449b447fc235be2023-09-02T13:54:09ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602016-01-011111515810.1590/0074-02760150376S0074-02762016000100051First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributesMaria Helena Galdino Figueiredo-CarvalhoLivia de Souza RamosLeonardo Silva BarbedoAlessandra Leal da Silva ChavesIlda Akemi MuramotoAndré Luis Souza dos SantosRodrigo Almeida-PaesRosely Maria Zancopé-OliveiraThis study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to fluconazole resistance. Adherence to inert surfaces was conducted onto glass and polystyrene. The biofilm formation and antifungal susceptibility on biofilm-growing cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal strains were able to bind both tested surfaces and form biofilm, with a binding preference to polystyrene (p < 0.001). AMB promoted significant reductions (≈50%) in biofilm production by our C. nivariensis strain using both methodologies. This reduction was also observed for CAS and POS, but only in the XTT assay. All strains were excellent protease producers and moderate phytase producers, but lipases were not detected. This study reinforces the pathogenic potential of C. nivariensis and its possible resistance profile to the azolic drugs generally used for candidiasis management.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100051&lng=en&tlng=enCandida nivariensisBrazilantifungal resistancehydrolytic enzymesadherencebiofilm |
| spellingShingle | Maria Helena Galdino Figueiredo-Carvalho Livia de Souza Ramos Leonardo Silva Barbedo Alessandra Leal da Silva Chaves Ilda Akemi Muramoto André Luis Souza dos Santos Rodrigo Almeida-Paes Rosely Maria Zancopé-Oliveira First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes Memorias do Instituto Oswaldo Cruz Candida nivariensis Brazil antifungal resistance hydrolytic enzymes adherence biofilm |
| title | First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
| title_full | First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
| title_fullStr | First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
| title_full_unstemmed | First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
| title_short | First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
| title_sort | first description of candida nivariensis in brazil antifungal susceptibility profile and potential virulence attributes |
| topic | Candida nivariensis Brazil antifungal resistance hydrolytic enzymes adherence biofilm |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100051&lng=en&tlng=en |
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