Integrin-Linked Kinase (ILK) Plays an Important Role in the Laminin-Dependent Development of Dorsal Root Ganglia during Chicken Embryogenesis
Integrin-linked kinase (ILK) is mainly localized in focal adhesions where it interacts and modulates the downstream signaling of integrins affecting cell migration, adhesion, and survival. The interaction of dorsal root ganglia (DRG) cells, being part of the peripheral nervous system (PNS), with the...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/7/1666 |
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| author | Ewa Mrówczyńska Antonina Joanna Mazur |
| author_facet | Ewa Mrówczyńska Antonina Joanna Mazur |
| author_sort | Ewa Mrówczyńska |
| collection | DOAJ |
| description | Integrin-linked kinase (ILK) is mainly localized in focal adhesions where it interacts and modulates the downstream signaling of integrins affecting cell migration, adhesion, and survival. The interaction of dorsal root ganglia (DRG) cells, being part of the peripheral nervous system (PNS), with the extracellular matrix (ECM) via integrins is crucial for proper PNS development. A few studies have focused on ILK’s role in PNS development, but none of these have focused on chicken. Therefore, we decided to investigate ILK’s role in the development of <i>Gallus gallus domesticus’s</i> DRG. First, using RT-PCR, Western blotting, and in situ hybridization, we show that <i>ILK</i> is expressed in DRG. Next, by immunocytochemistry, we show ILK’s localization both intracellularly and on the cell membrane of DRG neurons and Schwann cell precursors (SCPs). Finally, we describe ILK’s involvement in multiple aspects of DRG development by performing functional experiments in vitro. IgG-mediated interruption of ILK’s action improved DRG neurite outgrowth, modulated their directionality, stimulated SCPs migration, and impacted growth cone morphology in the presence of laminin-1 or laminin-1 mimicking peptide IKVAV. Taken together, our results show that ILK is important for chicken PNS development, probably via its exposure to the ECM. |
| first_indexed | 2024-03-10T09:43:02Z |
| format | Article |
| id | doaj.art-4974e4abd18f42c3bf81c333501d263b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T09:43:02Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-4974e4abd18f42c3bf81c333501d263b2023-11-22T03:28:03ZengMDPI AGCells2073-44092021-07-01107166610.3390/cells10071666Integrin-Linked Kinase (ILK) Plays an Important Role in the Laminin-Dependent Development of Dorsal Root Ganglia during Chicken EmbryogenesisEwa Mrówczyńska0Antonina Joanna Mazur1Department of Cell Pathology, Faculty of Biotechnology, University of Wroclaw, 50-383 Wroclaw, PolandDepartment of Cell Pathology, Faculty of Biotechnology, University of Wroclaw, 50-383 Wroclaw, PolandIntegrin-linked kinase (ILK) is mainly localized in focal adhesions where it interacts and modulates the downstream signaling of integrins affecting cell migration, adhesion, and survival. The interaction of dorsal root ganglia (DRG) cells, being part of the peripheral nervous system (PNS), with the extracellular matrix (ECM) via integrins is crucial for proper PNS development. A few studies have focused on ILK’s role in PNS development, but none of these have focused on chicken. Therefore, we decided to investigate ILK’s role in the development of <i>Gallus gallus domesticus’s</i> DRG. First, using RT-PCR, Western blotting, and in situ hybridization, we show that <i>ILK</i> is expressed in DRG. Next, by immunocytochemistry, we show ILK’s localization both intracellularly and on the cell membrane of DRG neurons and Schwann cell precursors (SCPs). Finally, we describe ILK’s involvement in multiple aspects of DRG development by performing functional experiments in vitro. IgG-mediated interruption of ILK’s action improved DRG neurite outgrowth, modulated their directionality, stimulated SCPs migration, and impacted growth cone morphology in the presence of laminin-1 or laminin-1 mimicking peptide IKVAV. Taken together, our results show that ILK is important for chicken PNS development, probably via its exposure to the ECM.https://www.mdpi.com/2073-4409/10/7/1666integrin-linked kinase (ILK)lamininperipheral nervous system (PNS)dorsal root ganglion (DRG)axonal outgrowthSchwann cell precursors (SCPs) |
| spellingShingle | Ewa Mrówczyńska Antonina Joanna Mazur Integrin-Linked Kinase (ILK) Plays an Important Role in the Laminin-Dependent Development of Dorsal Root Ganglia during Chicken Embryogenesis Cells integrin-linked kinase (ILK) laminin peripheral nervous system (PNS) dorsal root ganglion (DRG) axonal outgrowth Schwann cell precursors (SCPs) |
| title | Integrin-Linked Kinase (ILK) Plays an Important Role in the Laminin-Dependent Development of Dorsal Root Ganglia during Chicken Embryogenesis |
| title_full | Integrin-Linked Kinase (ILK) Plays an Important Role in the Laminin-Dependent Development of Dorsal Root Ganglia during Chicken Embryogenesis |
| title_fullStr | Integrin-Linked Kinase (ILK) Plays an Important Role in the Laminin-Dependent Development of Dorsal Root Ganglia during Chicken Embryogenesis |
| title_full_unstemmed | Integrin-Linked Kinase (ILK) Plays an Important Role in the Laminin-Dependent Development of Dorsal Root Ganglia during Chicken Embryogenesis |
| title_short | Integrin-Linked Kinase (ILK) Plays an Important Role in the Laminin-Dependent Development of Dorsal Root Ganglia during Chicken Embryogenesis |
| title_sort | integrin linked kinase ilk plays an important role in the laminin dependent development of dorsal root ganglia during chicken embryogenesis |
| topic | integrin-linked kinase (ILK) laminin peripheral nervous system (PNS) dorsal root ganglion (DRG) axonal outgrowth Schwann cell precursors (SCPs) |
| url | https://www.mdpi.com/2073-4409/10/7/1666 |
| work_keys_str_mv | AT ewamrowczynska integrinlinkedkinaseilkplaysanimportantroleinthelaminindependentdevelopmentofdorsalrootgangliaduringchickenembryogenesis AT antoninajoannamazur integrinlinkedkinaseilkplaysanimportantroleinthelaminindependentdevelopmentofdorsalrootgangliaduringchickenembryogenesis |