Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosa
Background and objective: Increasing antibiotic resistance in most bacteria has led to the development of natural antimicrobial compounds. The purpose of this study was to investigate the disinfection effect of Ajowan essential oil (EO) on the standard strain of Pseudomonas aeruginosa Methods: In t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Alborz University of Medical Sciences
2022-12-01
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| Series: | Muhandisī-i Bihdāsht-i Muḥīṭ |
| Subjects: | |
| Online Access: | http://jehe.abzums.ac.ir/article-1-904-en.pdf |
| _version_ | 1797676546810445824 |
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| author | Bahram Kamarehie Fatemeh Farash Beiranvand Mohsen Mohammadi Mohammad Amin Karami |
| author_facet | Bahram Kamarehie Fatemeh Farash Beiranvand Mohsen Mohammadi Mohammad Amin Karami |
| author_sort | Bahram Kamarehie |
| collection | DOAJ |
| description | Background and objective: Increasing antibiotic resistance in most bacteria has led to the development of natural antimicrobial compounds. The purpose of this study was to investigate the disinfection effect of Ajowan essential oil (EO) on the standard strain of Pseudomonas aeruginosa
Methods: In this study, the essential oil was prepared by distillation of Ajowan. The components of the essential oil were separated and identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Then the antibacterial effects of two combinations including Ajowan EO +dimethyl sulfoxide (DMSO) and glutaraldehyde 2% on standard strain of Pseudomonas aeruginosa were evaluated and determined using dilution broth and disk diffusion methods
Results: The results of the study showed that the most component of the essential oil consist of: Thymol (50%), P-cymene (23.6%), and Terpinen (20.3%). The mean values of MIC in the antibacterial substances (Ajowan EO+DMSO, Glutaraldehyde 2%) were 83 and 125µg/mL respectively. The mean values of MBC for abovementioned substances were 125 and 166 µg/mL respectively. The mean diameter of the inhibition zone in the MIC concentration was 11and 12.66 mm respectively.
Conclusion: According to the obtained results, Essential oils Ajowan has similar effect of glutaraldehyde 2% on Pseudomonas aeruginosa. It may be a possible substitute to glutaraldehyde in hospital disinfection purposes. |
| first_indexed | 2024-03-11T22:29:42Z |
| format | Article |
| id | doaj.art-4976de9c13b0462fa688c62df230cedb |
| institution | Directory Open Access Journal |
| issn | 2383-3211 |
| language | fas |
| last_indexed | 2024-03-11T22:29:42Z |
| publishDate | 2022-12-01 |
| publisher | Alborz University of Medical Sciences |
| record_format | Article |
| series | Muhandisī-i Bihdāsht-i Muḥīṭ |
| spelling | doaj.art-4976de9c13b0462fa688c62df230cedb2023-09-23T06:02:58ZfasAlborz University of Medical SciencesMuhandisī-i Bihdāsht-i Muḥīṭ2383-32112022-12-011017282Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosaBahram Kamarehie0Fatemeh Farash Beiranvand1Mohsen Mohammadi2Mohammad Amin Karami3 Environmental Health Research Center, Lorestan University of Medical sciences, Khorramabad, Iran Health Center of Khorramabad, Lorestan University of Medical sciences, Khorramabad, Iran Hepatitis Research Center Lorestan University of Medical Sciences, Khorramabad, Iran Environmental Health Research Center, Lorestan University of Medical sciences, Khorramabad, Iran Background and objective: Increasing antibiotic resistance in most bacteria has led to the development of natural antimicrobial compounds. The purpose of this study was to investigate the disinfection effect of Ajowan essential oil (EO) on the standard strain of Pseudomonas aeruginosa Methods: In this study, the essential oil was prepared by distillation of Ajowan. The components of the essential oil were separated and identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Then the antibacterial effects of two combinations including Ajowan EO +dimethyl sulfoxide (DMSO) and glutaraldehyde 2% on standard strain of Pseudomonas aeruginosa were evaluated and determined using dilution broth and disk diffusion methods Results: The results of the study showed that the most component of the essential oil consist of: Thymol (50%), P-cymene (23.6%), and Terpinen (20.3%). The mean values of MIC in the antibacterial substances (Ajowan EO+DMSO, Glutaraldehyde 2%) were 83 and 125µg/mL respectively. The mean values of MBC for abovementioned substances were 125 and 166 µg/mL respectively. The mean diameter of the inhibition zone in the MIC concentration was 11and 12.66 mm respectively. Conclusion: According to the obtained results, Essential oils Ajowan has similar effect of glutaraldehyde 2% on Pseudomonas aeruginosa. It may be a possible substitute to glutaraldehyde in hospital disinfection purposes.http://jehe.abzums.ac.ir/article-1-904-en.pdf disinfectantajowannosocomial infection |
| spellingShingle | Bahram Kamarehie Fatemeh Farash Beiranvand Mohsen Mohammadi Mohammad Amin Karami Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosa Muhandisī-i Bihdāsht-i Muḥīṭ disinfectant ajowan nosocomial infection |
| title | Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosa |
| title_full | Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosa |
| title_fullStr | Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosa |
| title_full_unstemmed | Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosa |
| title_short | Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosa |
| title_sort | evaluation of disinfection effect of ajowan on pseudomonas aeruginosa |
| topic | disinfectant ajowan nosocomial infection |
| url | http://jehe.abzums.ac.ir/article-1-904-en.pdf |
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