Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging
<p>Abstract</p> <p>Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modif...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2009-01-01
|
| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1007/s11671-008-9239-9 |
| _version_ | 1797722257575903232 |
|---|---|
| author | Nie Fang Teng Gaojun Ge Yuqing Zhang Yu He Shiying Gu Ning |
| author_facet | Nie Fang Teng Gaojun Ge Yuqing Zhang Yu He Shiying Gu Ning |
| author_sort | Nie Fang |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles’ concentration. The minimum detected number of labeled cells was around 10<sup>4</sup>by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.</p> |
| first_indexed | 2024-03-12T09:44:57Z |
| format | Article |
| id | doaj.art-497837c6df164cefb12d5a4092797c4d |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T09:44:57Z |
| publishDate | 2009-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-497837c6df164cefb12d5a4092797c4d2023-09-02T12:55:50ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2009-01-0144287295Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular ImagingNie FangTeng GaojunGe YuqingZhang YuHe ShiyingGu Ning<p>Abstract</p> <p>Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles’ concentration. The minimum detected number of labeled cells was around 10<sup>4</sup>by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.</p>http://dx.doi.org/10.1007/s11671-008-9239-9Magnetic nanoparticleFluorescenceChitosanMagnetic resonance imaging |
| spellingShingle | Nie Fang Teng Gaojun Ge Yuqing Zhang Yu He Shiying Gu Ning Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging Nanoscale Research Letters Magnetic nanoparticle Fluorescence Chitosan Magnetic resonance imaging |
| title | Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging |
| title_full | Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging |
| title_fullStr | Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging |
| title_full_unstemmed | Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging |
| title_short | Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging |
| title_sort | fluorescence modified chitosan coated magnetic nanoparticles for high efficient cellular imaging |
| topic | Magnetic nanoparticle Fluorescence Chitosan Magnetic resonance imaging |
| url | http://dx.doi.org/10.1007/s11671-008-9239-9 |
| work_keys_str_mv | AT niefang fluorescencemodifiedchitosancoatedmagneticnanoparticlesforhighefficientcellularimaging AT tenggaojun fluorescencemodifiedchitosancoatedmagneticnanoparticlesforhighefficientcellularimaging AT geyuqing fluorescencemodifiedchitosancoatedmagneticnanoparticlesforhighefficientcellularimaging AT zhangyu fluorescencemodifiedchitosancoatedmagneticnanoparticlesforhighefficientcellularimaging AT heshiying fluorescencemodifiedchitosancoatedmagneticnanoparticlesforhighefficientcellularimaging AT guning fluorescencemodifiedchitosancoatedmagneticnanoparticlesforhighefficientcellularimaging |