Development of an integrative database with 499 novel microsatellite markers for <it>Macaca fascicularis</it>
<p>Abstract</p> <p>Background</p> <p>Cynomolgus macaques (<it>Macaca fascicularis</it>) are a valuable resource for linkage studies of genetic disorders, but their microsatellite markers are not sufficient. In genetic studies, a prerequisite for mapping gene...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-06-01
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| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/10/24 |
| _version_ | 1818199876999053312 |
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| author | Higashino Atsunori Osada Naoki Suto Yumiko Hirata Makoto Kameoka Yosuke Takahashi Ichiro Terao Keiji |
| author_facet | Higashino Atsunori Osada Naoki Suto Yumiko Hirata Makoto Kameoka Yosuke Takahashi Ichiro Terao Keiji |
| author_sort | Higashino Atsunori |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cynomolgus macaques (<it>Macaca fascicularis</it>) are a valuable resource for linkage studies of genetic disorders, but their microsatellite markers are not sufficient. In genetic studies, a prerequisite for mapping genes is development of a genome-wide set of microsatellite markers in target organisms. A whole genome sequence and its annotation also facilitate identification of markers for causative mutations. The aim of this study is to establish hundreds of microsatellite markers and to develop an integrative cynomolgus macaque genome database with a variety of datasets including marker and gene information that will be useful for further genetic analyses in this species.</p> <p>Results</p> <p>We investigated the level of polymorphisms in cynomolgus monkeys for 671 microsatellite markers that are covered by our established Bacterial Artificial Chromosome (BAC) clones. Four hundred and ninety-nine (74.4%) of the markers were found to be polymorphic using standard PCR analysis. The average number of alleles and average expected heterozygosity at these polymorphic loci in ten cynomolgus macaques were 8.20 and 0.75, respectively.</p> <p>Conclusion</p> <p>BAC clones and novel microsatellite markers were assigned to the rhesus genome sequence and linked with our cynomolgus macaque cDNA database (QFbase). Our novel microsatellite marker set and genomic database will be valuable integrative resources in analyzing genetic disorders in cynomolgus macaques.</p> |
| first_indexed | 2024-12-12T02:28:44Z |
| format | Article |
| id | doaj.art-4978c5c38db741d7ab83d4fd836caf3a |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-12-12T02:28:44Z |
| publishDate | 2009-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-4978c5c38db741d7ab83d4fd836caf3a2022-12-22T00:41:28ZengBMCBMC Genetics1471-21562009-06-011012410.1186/1471-2156-10-24Development of an integrative database with 499 novel microsatellite markers for <it>Macaca fascicularis</it>Higashino AtsunoriOsada NaokiSuto YumikoHirata MakotoKameoka YosukeTakahashi IchiroTerao Keiji<p>Abstract</p> <p>Background</p> <p>Cynomolgus macaques (<it>Macaca fascicularis</it>) are a valuable resource for linkage studies of genetic disorders, but their microsatellite markers are not sufficient. In genetic studies, a prerequisite for mapping genes is development of a genome-wide set of microsatellite markers in target organisms. A whole genome sequence and its annotation also facilitate identification of markers for causative mutations. The aim of this study is to establish hundreds of microsatellite markers and to develop an integrative cynomolgus macaque genome database with a variety of datasets including marker and gene information that will be useful for further genetic analyses in this species.</p> <p>Results</p> <p>We investigated the level of polymorphisms in cynomolgus monkeys for 671 microsatellite markers that are covered by our established Bacterial Artificial Chromosome (BAC) clones. Four hundred and ninety-nine (74.4%) of the markers were found to be polymorphic using standard PCR analysis. The average number of alleles and average expected heterozygosity at these polymorphic loci in ten cynomolgus macaques were 8.20 and 0.75, respectively.</p> <p>Conclusion</p> <p>BAC clones and novel microsatellite markers were assigned to the rhesus genome sequence and linked with our cynomolgus macaque cDNA database (QFbase). Our novel microsatellite marker set and genomic database will be valuable integrative resources in analyzing genetic disorders in cynomolgus macaques.</p>http://www.biomedcentral.com/1471-2156/10/24 |
| spellingShingle | Higashino Atsunori Osada Naoki Suto Yumiko Hirata Makoto Kameoka Yosuke Takahashi Ichiro Terao Keiji Development of an integrative database with 499 novel microsatellite markers for <it>Macaca fascicularis</it> BMC Genetics |
| title | Development of an integrative database with 499 novel microsatellite markers for <it>Macaca fascicularis</it> |
| title_full | Development of an integrative database with 499 novel microsatellite markers for <it>Macaca fascicularis</it> |
| title_fullStr | Development of an integrative database with 499 novel microsatellite markers for <it>Macaca fascicularis</it> |
| title_full_unstemmed | Development of an integrative database with 499 novel microsatellite markers for <it>Macaca fascicularis</it> |
| title_short | Development of an integrative database with 499 novel microsatellite markers for <it>Macaca fascicularis</it> |
| title_sort | development of an integrative database with 499 novel microsatellite markers for it macaca fascicularis it |
| url | http://www.biomedcentral.com/1471-2156/10/24 |
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