Specific and Global RNA Regulators in <i>Pseudomonas aeruginosa</i>
<i>Pseudomonas aeruginosa</i> (<i>Pae</i>) is an opportunistic pathogen showing a high intrinsic resistance to a wide variety of antibiotics. It causes nosocomial infections that are particularly detrimental to immunocompromised individuals and to patients suffering from cyst...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/16/8632 |
| _version_ | 1797523560584970240 |
|---|---|
| author | Petra Pusic Elisabeth Sonnleitner Udo Bläsi |
| author_facet | Petra Pusic Elisabeth Sonnleitner Udo Bläsi |
| author_sort | Petra Pusic |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> (<i>Pae</i>) is an opportunistic pathogen showing a high intrinsic resistance to a wide variety of antibiotics. It causes nosocomial infections that are particularly detrimental to immunocompromised individuals and to patients suffering from cystic fibrosis. We provide a snapshot on regulatory RNAs of <i>Pae</i> that impact on metabolism, pathogenicity and antibiotic susceptibility. Different experimental approaches such as in silico predictions, co-purification with the RNA chaperone Hfq as well as high-throughput RNA sequencing identified several hundreds of regulatory RNA candidates in <i>Pae</i>. Notwithstanding, using in vitro and in vivo assays, the function of only a few has been revealed. Here, we focus on well-characterized small base-pairing RNAs, regulating specific target genes as well as on larger protein-binding RNAs that sequester and thereby modulate the activity of translational repressors. As the latter impact large gene networks governing metabolism, acute or chronic infections, these protein-binding RNAs in conjunction with their cognate proteins are regarded as global post-transcriptional regulators. |
| first_indexed | 2024-03-10T08:44:42Z |
| format | Article |
| id | doaj.art-4978ceb18c44426392dd7c4f64dfc349 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:42Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4978ceb18c44426392dd7c4f64dfc3492023-11-22T07:58:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216863210.3390/ijms22168632Specific and Global RNA Regulators in <i>Pseudomonas aeruginosa</i>Petra Pusic0Elisabeth Sonnleitner1Udo Bläsi2Max Perutz Labs, Department of Microbiology, Immunobiology and Genetics, Centre of Molecular Biology, Vienna Biocenter (VBC), University of Vienna, Dr. Bohrgasse 9/4, 1030 Vienna, AustriaMax Perutz Labs, Department of Microbiology, Immunobiology and Genetics, Centre of Molecular Biology, Vienna Biocenter (VBC), University of Vienna, Dr. Bohrgasse 9/4, 1030 Vienna, AustriaMax Perutz Labs, Department of Microbiology, Immunobiology and Genetics, Centre of Molecular Biology, Vienna Biocenter (VBC), University of Vienna, Dr. Bohrgasse 9/4, 1030 Vienna, Austria<i>Pseudomonas aeruginosa</i> (<i>Pae</i>) is an opportunistic pathogen showing a high intrinsic resistance to a wide variety of antibiotics. It causes nosocomial infections that are particularly detrimental to immunocompromised individuals and to patients suffering from cystic fibrosis. We provide a snapshot on regulatory RNAs of <i>Pae</i> that impact on metabolism, pathogenicity and antibiotic susceptibility. Different experimental approaches such as in silico predictions, co-purification with the RNA chaperone Hfq as well as high-throughput RNA sequencing identified several hundreds of regulatory RNA candidates in <i>Pae</i>. Notwithstanding, using in vitro and in vivo assays, the function of only a few has been revealed. Here, we focus on well-characterized small base-pairing RNAs, regulating specific target genes as well as on larger protein-binding RNAs that sequester and thereby modulate the activity of translational repressors. As the latter impact large gene networks governing metabolism, acute or chronic infections, these protein-binding RNAs in conjunction with their cognate proteins are regarded as global post-transcriptional regulators.https://www.mdpi.com/1422-0067/22/16/8632<i>Pseudomonas aeruginosa</i>base-pairing sRNAsprotein-binding RNAsHfqCrcRsmA |
| spellingShingle | Petra Pusic Elisabeth Sonnleitner Udo Bläsi Specific and Global RNA Regulators in <i>Pseudomonas aeruginosa</i> International Journal of Molecular Sciences <i>Pseudomonas aeruginosa</i> base-pairing sRNAs protein-binding RNAs Hfq Crc RsmA |
| title | Specific and Global RNA Regulators in <i>Pseudomonas aeruginosa</i> |
| title_full | Specific and Global RNA Regulators in <i>Pseudomonas aeruginosa</i> |
| title_fullStr | Specific and Global RNA Regulators in <i>Pseudomonas aeruginosa</i> |
| title_full_unstemmed | Specific and Global RNA Regulators in <i>Pseudomonas aeruginosa</i> |
| title_short | Specific and Global RNA Regulators in <i>Pseudomonas aeruginosa</i> |
| title_sort | specific and global rna regulators in i pseudomonas aeruginosa i |
| topic | <i>Pseudomonas aeruginosa</i> base-pairing sRNAs protein-binding RNAs Hfq Crc RsmA |
| url | https://www.mdpi.com/1422-0067/22/16/8632 |
| work_keys_str_mv | AT petrapusic specificandglobalrnaregulatorsinipseudomonasaeruginosai AT elisabethsonnleitner specificandglobalrnaregulatorsinipseudomonasaeruginosai AT udoblasi specificandglobalrnaregulatorsinipseudomonasaeruginosai |