Microbial community composition and function in an urban waterway with combined sewer overflows before and after implementation of a stormwater storage pipe
When the wastewater volume exceeds the sewer pipe capacity during extreme rainfall events, untreated sewage discharges directly into rivers as combined sewer overflow (CSO). To compare the impacts of CSOs and stormwater on urban waterways, we assessed physicochemical water quality, the 16S rRNA gene...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2023-01-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/14684.pdf |
| _version_ | 1797420328635334656 |
|---|---|
| author | Kazuaki Matsui Takeshi Miki |
| author_facet | Kazuaki Matsui Takeshi Miki |
| author_sort | Kazuaki Matsui |
| collection | DOAJ |
| description | When the wastewater volume exceeds the sewer pipe capacity during extreme rainfall events, untreated sewage discharges directly into rivers as combined sewer overflow (CSO). To compare the impacts of CSOs and stormwater on urban waterways, we assessed physicochemical water quality, the 16S rRNA gene-based bacterial community structure, and EcoPlate-based microbial functions during rainfall periods in an urban waterway before and after a stormwater storage pipe was commissioned. A temporal variation analysis showed that CSOs have significant impacts on microbial function and bacterial community structure, while their contributions to physicochemical parameters, bacterial abundance, and chlorophyll a were not confirmed. Heat map analysis showed that the impact of CSO on the waterway bacterial community structure was temporal and the bacterial community composition in CSO is distinct from that in sewers. Hierarchical clustering analysis revealed that the waterway physicochemical water qualities, bacterial community composition, and microbial community function were distinguishable from the upper reach of the river, rather than between CSO and stormwater. Changes in the relative abundance of tetracycline resistance (tet) genes—especially tet(M)—were observed after CSOs but did not coincide with changes in the microbial community composition, suggesting that the parameters affecting the microbial community composition and relative abundance of tet genes differ. After pipe implementation, however, stormwater did not contribute to the abundance of tet genes in the waterway. These results indicate that CSO-induced acute microbial disturbances in the urban waterway were alleviated by the implementation of a stormwater storage pipe and will support the efficiency of storage pipe operation for waterway management in urban areas. |
| first_indexed | 2024-03-09T06:59:56Z |
| format | Article |
| id | doaj.art-4979a4bbcc204896808765bffac1b865 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:59:56Z |
| publishDate | 2023-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-4979a4bbcc204896808765bffac1b8652023-12-03T09:53:36ZengPeerJ Inc.PeerJ2167-83592023-01-0111e1468410.7717/peerj.14684Microbial community composition and function in an urban waterway with combined sewer overflows before and after implementation of a stormwater storage pipeKazuaki Matsui0Takeshi Miki1Department of Civil and Environmental Engineering, Kindai University, Higashiosaka, JapanFaculty of Advanced Science and Technology, Ryukoku University, Otsu, Shiga, JapanWhen the wastewater volume exceeds the sewer pipe capacity during extreme rainfall events, untreated sewage discharges directly into rivers as combined sewer overflow (CSO). To compare the impacts of CSOs and stormwater on urban waterways, we assessed physicochemical water quality, the 16S rRNA gene-based bacterial community structure, and EcoPlate-based microbial functions during rainfall periods in an urban waterway before and after a stormwater storage pipe was commissioned. A temporal variation analysis showed that CSOs have significant impacts on microbial function and bacterial community structure, while their contributions to physicochemical parameters, bacterial abundance, and chlorophyll a were not confirmed. Heat map analysis showed that the impact of CSO on the waterway bacterial community structure was temporal and the bacterial community composition in CSO is distinct from that in sewers. Hierarchical clustering analysis revealed that the waterway physicochemical water qualities, bacterial community composition, and microbial community function were distinguishable from the upper reach of the river, rather than between CSO and stormwater. Changes in the relative abundance of tetracycline resistance (tet) genes—especially tet(M)—were observed after CSOs but did not coincide with changes in the microbial community composition, suggesting that the parameters affecting the microbial community composition and relative abundance of tet genes differ. After pipe implementation, however, stormwater did not contribute to the abundance of tet genes in the waterway. These results indicate that CSO-induced acute microbial disturbances in the urban waterway were alleviated by the implementation of a stormwater storage pipe and will support the efficiency of storage pipe operation for waterway management in urban areas.https://peerj.com/articles/14684.pdfCSOs16S rRNA gene sequencingEcoPlateTetracycline-resistance genesWaterway restoration |
| spellingShingle | Kazuaki Matsui Takeshi Miki Microbial community composition and function in an urban waterway with combined sewer overflows before and after implementation of a stormwater storage pipe PeerJ CSOs 16S rRNA gene sequencing EcoPlate Tetracycline-resistance genes Waterway restoration |
| title | Microbial community composition and function in an urban waterway with combined sewer overflows before and after implementation of a stormwater storage pipe |
| title_full | Microbial community composition and function in an urban waterway with combined sewer overflows before and after implementation of a stormwater storage pipe |
| title_fullStr | Microbial community composition and function in an urban waterway with combined sewer overflows before and after implementation of a stormwater storage pipe |
| title_full_unstemmed | Microbial community composition and function in an urban waterway with combined sewer overflows before and after implementation of a stormwater storage pipe |
| title_short | Microbial community composition and function in an urban waterway with combined sewer overflows before and after implementation of a stormwater storage pipe |
| title_sort | microbial community composition and function in an urban waterway with combined sewer overflows before and after implementation of a stormwater storage pipe |
| topic | CSOs 16S rRNA gene sequencing EcoPlate Tetracycline-resistance genes Waterway restoration |
| url | https://peerj.com/articles/14684.pdf |
| work_keys_str_mv | AT kazuakimatsui microbialcommunitycompositionandfunctioninanurbanwaterwaywithcombinedseweroverflowsbeforeandafterimplementationofastormwaterstoragepipe AT takeshimiki microbialcommunitycompositionandfunctioninanurbanwaterwaywithcombinedseweroverflowsbeforeandafterimplementationofastormwaterstoragepipe |