The temporal expression pattern of classical MHC class I in sleep-restricted mice: Generalizations and broader implications
The intricate relationship between sleep and leukocyte trafficking has garnered intense attention, particularly their homing dynamics to secondary lymphoid organs under normal and restricted sleep (SR). Considering the scarcity of information regarding circadian rhythms in major histocompatibility c...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Brain, Behavior, & Immunity - Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666354624000292 |
| _version_ | 1797221690315374592 |
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| author | Colette S. Kabrita Samar Al Bitar Esther Ghanem |
| author_facet | Colette S. Kabrita Samar Al Bitar Esther Ghanem |
| author_sort | Colette S. Kabrita |
| collection | DOAJ |
| description | The intricate relationship between sleep and leukocyte trafficking has garnered intense attention, particularly their homing dynamics to secondary lymphoid organs under normal and restricted sleep (SR). Considering the scarcity of information regarding circadian rhythms in major histocompatibility class I (MHC-I) expression in SR, we designed a study that assessed the temporal expression of MHC-I in murine lymph nodes and spleen and the subsequent effects of sleep recovery. Male C57BL/6, housed in 12:12 light/dark cycle, were grouped into control (C) and SR. SR was carried for one week before lymphoid tissues were sampled at selected time points and assessed for leukocyte number and MHC-I expression.SR resulted in 21% decrease in granulocyte and 24% increase in agranulocyte numbers.In C, MHC-I expression pattern in lymph nodes was bimodal and relatively higher than splenocytes during the animal’s active phase (110.2 ± 1.8 vs 81.9 ± 3.8, respectively; p = 0.002). Splenocytes; however, showed a bimodal pattern upon SR, with higher protein levels during the rest than the activity period (154.6 + 36.2 vs 99.5 + 15.9, respectively; p = 0.002), suggesting preparedness for a potential infection. Furthermore, SR caused a significant drop in MHC-I expression at the onset of rest with 57% and 30% reduction in lymph nodes and splenocytes, respectively. However, the overall protein expression collectively taken from both lymphoid tissues remained stable, emphasizing its indispensable role in immunological homeostasis. This stability coincided with the restoration of protein levels to baseline after a short sleep recovery period, resembling a reset for MHC-I antigen presentation following a week of SR.Understanding the interplay between MHC-I expression and contextual factors could enhance treatment protocols, refining the efficacy and time precision of glucocorticoid-based therapies in immune modulation. |
| first_indexed | 2024-04-24T13:09:26Z |
| format | Article |
| id | doaj.art-497b81a10a57470f958ea2233be67d54 |
| institution | Directory Open Access Journal |
| issn | 2666-3546 |
| language | English |
| last_indexed | 2024-04-24T13:09:26Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain, Behavior, & Immunity - Health |
| spelling | doaj.art-497b81a10a57470f958ea2233be67d542024-04-05T04:41:40ZengElsevierBrain, Behavior, & Immunity - Health2666-35462024-05-0137100751The temporal expression pattern of classical MHC class I in sleep-restricted mice: Generalizations and broader implicationsColette S. Kabrita0Samar Al Bitar1Esther Ghanem2Department of Sciences, Faculty of Natural and Applied Sciences, Notre Dame University-Louaize, Zouk Mosbeh, LebanonDepartment of Sciences, Faculty of Natural and Applied Sciences, Notre Dame University-Louaize, Zouk Mosbeh, LebanonCorresponding author. Department of Sciences, Faculty of Natural and Applied Sciences, Notre Dame University-Louaize, P.O. Box 72, Zouk Mikael, Zouk Mosbeh, Lebanon.; Department of Sciences, Faculty of Natural and Applied Sciences, Notre Dame University-Louaize, Zouk Mosbeh, LebanonThe intricate relationship between sleep and leukocyte trafficking has garnered intense attention, particularly their homing dynamics to secondary lymphoid organs under normal and restricted sleep (SR). Considering the scarcity of information regarding circadian rhythms in major histocompatibility class I (MHC-I) expression in SR, we designed a study that assessed the temporal expression of MHC-I in murine lymph nodes and spleen and the subsequent effects of sleep recovery. Male C57BL/6, housed in 12:12 light/dark cycle, were grouped into control (C) and SR. SR was carried for one week before lymphoid tissues were sampled at selected time points and assessed for leukocyte number and MHC-I expression.SR resulted in 21% decrease in granulocyte and 24% increase in agranulocyte numbers.In C, MHC-I expression pattern in lymph nodes was bimodal and relatively higher than splenocytes during the animal’s active phase (110.2 ± 1.8 vs 81.9 ± 3.8, respectively; p = 0.002). Splenocytes; however, showed a bimodal pattern upon SR, with higher protein levels during the rest than the activity period (154.6 + 36.2 vs 99.5 + 15.9, respectively; p = 0.002), suggesting preparedness for a potential infection. Furthermore, SR caused a significant drop in MHC-I expression at the onset of rest with 57% and 30% reduction in lymph nodes and splenocytes, respectively. However, the overall protein expression collectively taken from both lymphoid tissues remained stable, emphasizing its indispensable role in immunological homeostasis. This stability coincided with the restoration of protein levels to baseline after a short sleep recovery period, resembling a reset for MHC-I antigen presentation following a week of SR.Understanding the interplay between MHC-I expression and contextual factors could enhance treatment protocols, refining the efficacy and time precision of glucocorticoid-based therapies in immune modulation.http://www.sciencedirect.com/science/article/pii/S2666354624000292C57BL/6 miceCircadian rhythmsSleep restrictionLeukocytesMajor histocompatibility complex class I |
| spellingShingle | Colette S. Kabrita Samar Al Bitar Esther Ghanem The temporal expression pattern of classical MHC class I in sleep-restricted mice: Generalizations and broader implications Brain, Behavior, & Immunity - Health C57BL/6 mice Circadian rhythms Sleep restriction Leukocytes Major histocompatibility complex class I |
| title | The temporal expression pattern of classical MHC class I in sleep-restricted mice: Generalizations and broader implications |
| title_full | The temporal expression pattern of classical MHC class I in sleep-restricted mice: Generalizations and broader implications |
| title_fullStr | The temporal expression pattern of classical MHC class I in sleep-restricted mice: Generalizations and broader implications |
| title_full_unstemmed | The temporal expression pattern of classical MHC class I in sleep-restricted mice: Generalizations and broader implications |
| title_short | The temporal expression pattern of classical MHC class I in sleep-restricted mice: Generalizations and broader implications |
| title_sort | temporal expression pattern of classical mhc class i in sleep restricted mice generalizations and broader implications |
| topic | C57BL/6 mice Circadian rhythms Sleep restriction Leukocytes Major histocompatibility complex class I |
| url | http://www.sciencedirect.com/science/article/pii/S2666354624000292 |
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